Título
Serotonin and 5-hydroxy-indole-acetic acid contents in dorsal raphe and suprachiasmatic nuclei in normal, malnourished and rehabilitated rats under 24 h of sleep deprivation.
Autor
ALFONSO BENITO ALFARO RODRIGUEZ
RIGOBERTO GONZALEZ PIÑA
ANGELICA GONZALEZ MACIEL
EMILIO ARCH TIRADO
Nivel de Acceso
Acceso Abierto
Materias
MEDICINA Y CIENCIAS DE LA SALUD - (CTI) Conducta animal - Ratas - ([Brain Research 1110(1):95 - 101]) Cromatografía líquida de alta presión - Métodos - Ratas - ([Brain Research 1110(1):95 - 101]) Ácido hidroxiindolacético - Metabolismo - Ratas - ([Brain Research 1110(1):95 - 101]) Desnutrición - Metabolismo - Ratas - ([Brain Research 1110(1):95 - 101]) Desnutrición - Patología - Ratas - ([Brain Research 1110(1):95 - 101]) Desnutrición - Rehabilitación - Ratas - ([Brain Research 1110(1):95 - 101]) Núcleos del rafe - Metabolismo - Ratas - ([Brain Research 1110(1):95 - 101]) Sprague - Dawley - Ratas - ([Brain Research 1110(1):95 - 101]) Serotonina - Metabolismo - Ratas - ([Brain Research 1110(1):95 - 101]) Privación de sueño - Metabolismo - Ratas - ([Brain Research 1110(1):95 - 101]) Núcleo supraquiasmático - Metabolismo - Ratas - ([Brain Research 1110(1):95 - 101]) Behavior, animal - Rats - ([Brain Research 1110(1):95 - 101]) Chromatography, high pressure liquid - Methods - Rats - ([Brain Research 1110(1):95 - 101]) Hydroxyindoleacetic acid - Metabolism - Rats - ([Brain Research 1110(1):95 - 101]) Malnutrition - Metabolism - Rats - ([Brain Research 1110(1):95 - 101]) Malnutrition - Pathology - Rats - ([Brain Research 1110(1):95 - 101]) Malnutrition - Rehabilitation - Rats - ([Brain Research 1110(1):95 - 101]) Raphe nuclei metabolism - Rats - ([Brain Research 1110(1):95 - 101]) Serotonin - Metabolism - Rats - ([Brain Research 1110(1):95 - 101]) Sleep deprivation - Metabolism - Rats - ([Brain Research 1110(1):95 - 101]) Suprachiasmatic nucleus - Metabolism - Rats - ([Brain Research 1110(1):95 - 101]) Serotonina - ([Brain Research 1110(1):95 - 101]) Acido 5-Hidroxi-Indol-Acético - ([Brain Research 1110(1):95 - 101]) Dorsal núcleos del rafe - ([Brain Research 1110(1):95 - 101]) Núcleo supraquiasmático - ([Brain Research 1110(1):95 - 101]) Privación del sueño, ratas - ([Brain Research 1110(1):95 - 101]) Serotonin - ([Brain Research 1110(1):95 - 101]) 5-Hydroxy-Indole-Acetic Acid - ([Brain Research 1110(1):95 - 101])
Resumen o descripción
It has been discussed that serotonin (5-HT) could be involved in the effects of sleep deprivation (SD) and/or malnutrition (M) on the sleep-wake cycle. The aim of this work was to study the effects of the M, SD and its interaction on 5-HT and 5-hydroxy-indole-acetic acid (5-HIAA) contents in the dorsal raphe (DR) and the suprachiasmatic nuclei (SCN), two sleep-wake cycle regulators. Forty-eight puppets rats were obtained from mothers fed with low or normal casein diet. They were allocated in 3 groups (n = 16 each): prenatal/postnatal casein malnutrition (6/6%), prenatal casein malnutrition/nutritional casein rehabilitation (6/25%) and prenatal/postnatal casein well-nourished state (25/25%). When rats were 60 days old, 24 animals were exposed to sleep deprivation by means of forced locomotion during 24 h. The remaining 24 were kept under normal conditions of sleep-wake cycle. Then, all animals were sacrificed by decapitation. DR and SCN were dissected and processed to determine the 5-HT and 5-HIAA contents by means of HPLC. It was observed that 6/6% rats showed a 5-HT increase (DR p < 0.011; SCN p < 0.019) as well as in SD (DR p < 0.0008; SCN p < 0.0009) with respect to 25/25% rats. No differences were found in 6/25% rats. Therefore, 5-HIAA decreased significantly in both nuclei in all the groups, notably in M + SD animals (DR p < 0.001; SCN p < 0.001). We conclude that the sleep-wake cycle disruptions produced by chronic M and SD are mediated in part by a synergistic effect on 5-HT in the DR-SCN pathway, perhaps due to a delay in the development of such brain structures. © 2006 Elsevier B.V. All rights reserved.
Editor
Elsevier
Fecha de publicación
2006
Tipo de publicación
Artículo
Recurso de información
http://repositorio.pediatria.gob.mx:8180/handle/20.500.12103/3122
10.1016/j.brainres.2006.06.069
Formato
application/pdf
Fuente
Brain Research 1110(1):95 - 101
Idioma
Inglés
Repositorio Orígen
Repositorio del Instituto Nacional de Pediatría
Descargas
544