Título
Metabolic activation of herbicide products by Vicia faba detected in human peripheral lymphocytes using alkaline single cell gel electrophoresis.
Autor
MARIA ELENA CALDERON SEGURA
BERTHA MOLINA ALVAREZ
Nivel de Acceso
Acceso Abierto
Materias
MEDICINA Y CIENCIAS DE LA SALUD - (CTI) Supervivencia celular - Efectos de drogas - ([Toxicol in Vitro 21(6):1143-1154]) Herbicidas - Metabolismo - ([Toxicol in Vitro 21(6):1143-1154]) Herbicidas - Toxicidad - ([Toxicol in Vitro 21(6):1143-1154]) Linfocitos - Efectos de drogasas - ([Toxicol in Vitro 21(6):1143-1154]) Linfocitos - Fisiología - ([Toxicol in Vitro 21(6):1143-1154]) Mutágenos - Metabolismo - ([Toxicol in Vitro 21(6):1143-1154]) Mutágenos - Toxicidad - ([Toxicol in Vitro 21(6):1143-1154]) Extractos vegetales - Farmacología - ([Toxicol in Vitro 21(6):1143-1154]) Raíces de plantas - Metabolismo - ([Toxicol in Vitro 21(6):1143-1154]) Tiocarbamatos - Toxicidad - ([Toxicol in Vitro 21(6):1143-1154]) Triazinas - Metabolismo - ([Toxicol in Vitro 21(6):1143-1154]) Triazinas - Toxicidad - ([Toxicol in Vitro 21(6):1143-1154]) Vicia faba - Metabolismo - ([Toxicol in Vitro 21(6):1143-1154]) Cell Survival - Drug effects - ([Toxicol in Vitro 21(6):1143-1154]) Herbicides - Metabolism - ([Toxicol in Vitro 21(6):1143-1154]) Herbicides - Toxicity - ([Toxicol in Vitro 21(6):1143-1154]) Triazines - Metabolism - ([Toxicol in Vitro 21(6):1143-1154]) Triazines - Toxicity - ([Toxicol in Vitro 21(6):1143-1154]) Lymphocytes drug effects - ([Toxicol in Vitro 21(6):1143-1154]) Lymphocytes - Physiology - ([Toxicol in Vitro 21(6):1143-1154]) Mutagens - Metabolism - ([Toxicol in Vitro 21(6):1143-1154]) Mutagens - Toxicity - ([Toxicol in Vitro 21(6):1143-1154]) Plant extracts - Pharmacology - ([Toxicol in Vitro 21(6):1143-1154]) Plant roots - Metabolism - ([Toxicol in Vitro 21(6):1143-1154]) Thiocarbamates - Toxicity - ([Toxicol in Vitro 21(6):1143-1154]) Vicia faba - Metabolism - ([Toxicol in Vitro 21(6):1143-1154]) Ametryn - ([Toxicol in Vitro 21(6):1143-1154]) Metribuzin - ([Toxicol in Vitro 21(6):1143-1154]) EPTC herbicides - ([Toxicol in Vitro 21(6):1143-1154]) Human peripheral lymphocytes - ([Toxicol in Vitro 21(6):1143-1154]) In vivo vicia faba metabolic activation - ([Toxicol in Vitro 21(6):1143-1154]) Comet tail length - ([Toxicol in Vitro 21(6):1143-1154]) DNA damaged cells - ([Toxicol in Vitro 21(6):1143-1154])
Resumen o descripción
Ametryn and metribuzin S-triazines derivatives and EPTC thiocarbamate are herbicides used extensively in Mexican agriculture, for
example in crops such as corn, sugar cane, tomato, wheat, and beans. The present study evaluated the DNA damage and cytotoxic effects
of three herbicides after metabolism by Vicia faba roots in human peripheral lymphocytes using akaline single cell gel electrophoresis.
Three parameters were scored as indicators of DNA damage: tail length, percentage of cells with DNA damage (with comet), and level
DNA damage. The lymphocytes were treated for 2 h with 0.5–5.0 lg/ml ametryn or metribuzin and 1.5–10 lg/ml EPTC. Lymphocytes
also were coincubated for 2 h with 20 ll V. faba roots extracts that had been treated for 4 h with 50–500 mg/l of the two triazines or with
the thiocarbamate herbicide or with ethanol (3600 mg/l), as positive control. The lymphocytes treated with three pesticides without in vivo
metabolic activation by V. faba root did not show significant differences in the mean values bewteen genotoxic parameters compared with
negative control. But when human cells were exposed to three herbicides after they had been metabolized the frequency of cell comet, tail
length and level DNA damage all increased. At highest concentrations of the three herbicides produced severe DNA damage compared
with S10 fraction and negative control. The linear regression analysis of the tail length values of three herbicides indicated that there was
genotoxic effect concentration-response relationship with ametryn and ametribuzin but no EPTC. The ethanol induced major increase
DNA damage compared with S10 fraction and the three pesticides. There were not effects in cell viability with treatment EPTC and metribuzin
wherther or not it had been metabolized. High concentrations of ametryn alone and after it had been metabolized decreased cell viability
compared with the negative control. The results demonstrated that the three herbicides needed to be activated by the V. faba root
metabolism to produce DNA damage in human peripheral lymphocyte. The alkaline comet technique is a rapid and sensitive assay, to
quickly evaluate DNA damage the metabolic activation of herbicide products by V. faba root in human cells in vitro.
Editor
Pergamon Press
Fecha de publicación
2007
Tipo de publicación
Artículo
Recurso de información
http://repositorio.pediatria.gob.mx:8180/handle/20.500.12103/3128
10.1016/j.tiv.2007.03.002
Formato
application/pdf
Fuente
Toxicol in Vitro 21(6):1143-1154
Idioma
Inglés
Repositorio Orígen
Repositorio del Instituto Nacional de Pediatría
Descargas
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