Título
Efecto de la infección del virus de Zika en la diferenciación neural de una línea celular troncal humana.
Astrocytes derived from Neural Progenitor Cells are susceptible to Zika Virus Infection
Autor
EDSON IVAN RUBIO HERNANDEZ
Colaborador
CHRISTIAN ALBERTO GARCIA SEPULVEDA
JOSE FERNANDO PEÑA ORTEGA
Nivel de Acceso
Acceso Abierto
Resumen o descripción
Zika virus (ZIKV) was first isolated in 1947. From its isolation until 2007, symptoms
of ZIKV-caused disease were limited (e.g., fever, hives, and headache); however,
during the epidemic in Brazil in 2014, ZIKV infection caused Guillain-Barré syndrome
in adults and microcephaly in fetuses and infants of women infected during
pregnancy. The neurovirulence of ZIKV has been studied using neural progenitor
cells (NPCs), brain organoids, neurons, and astrocytes. NPCs and astrocytes
appear to be the most susceptible cells of the Central Nervous System to ZIKV
infection. In this work, we aimed to develop a culture of astrocytes derived from a
human NPC cell line. We analyze how ZIKV affects human astrocytes and
demonstrate that 1) ZIKV infection reduces cell viability, increases the production of
Reactive Oxygen Species (ROS), and results in high viral titers; 2) there are changes
in the expression of genes that facilitate the entry of the virus into the cells; 3) there
are changes in the expression of genes involved in the homeostasis of the
glutamatergic system; and 4) there are ultrastructural changes in mitochondria and
lipid droplets associated with production of virions. Our findings reveal new evidence
of how ZIKV compromises astrocytic functionality, which may help understand the
pathophysiology of ZIKV-associated congenital disease.
Editor
Facultad de Medicina
Fecha de publicación
marzo de 2023
Tipo de publicación
Tesis de doctorado
Versión de la publicación
Versión publicada
Recurso de información
Formato
application/pdf
Idioma
Español
Cobertura
México. San Luis Potosí. San Luis Potosí
Repositorio Orígen
Repositorio Institucional NINIVE
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