Título

Effect of exogenous xylanase on rumen in vitro gas production and degradability of wheat straw

Autor

Togtokhbayar Norovsambuu

MARIA ANDREA CERRILLO SOTO

GERMAN BUENDIA RODRIGUEZ

MONA MOHAMED MOHAMED YASSEEN ELGHANDOUR

Abdelfattah Zeidan Mohamed Salem

URANKHAICH CHULUUNBAATAR

NICHOLAS E. ODONGO

Ahmed E. Kholif

Nivel de Acceso

Acceso Abierto

Licencia

http://creativecommons.org/licenses/by-nc-nd/4.0

Materias

degradability gas production wheat straw xylanase BIOLOGÍA Y QUÍMICA - (CTI)

Resumen o descripción

In Mongolia, the low and seasonal yield of native pastures causes a feed gap between the annual pasture provision and the requirements of grazing ruminants. Therefore, there is a growing need to improve the feeding value of locally available resources such as wheat straw for supplementary feeding. Wheat straw constitutes an important source of roughage for ruminants. It typically contain more than 70% neutral detergent fiber (NDF) on a dry matter (DM) basis, with less than 40% total digestive tract digestibility of the NDF, even under ideal feeding conditions (NRC 2001).

The objective of this study was to determine effects of xylanase on in vitro gas production (GP) and in sacco degradability of wheat straw. Rumen fluid was obtained from three Mongolian native goats fitted with permanent rumen cannulas. The trial consisted of five doses (0, 0.5, 1.0, 1.5, 2.0 μL/g of substrate) of a commercial xylanase (Dyadic® xylanase PLUS, Dyadic International, Inc., Jupiter, FL, USA). For the in sacco degradability, different levels of xylanase enzyme were added directly onto 2 g of wheat straw in nylon bags and incubated in the rumen for 3, 6, 12, 24 and 48 h to estimate degradability of wheat straw. Total GP increased (P < 0.001) at all times of incubation at intermediate levels of xylanase. Methane production had a similar pattern at 3 and 12 h of incubation; increased linearly at 24 h of incubation, and was unaffected at 6 and 48 h of incubation. Rumen NH3-N concentration increased linearly at 3 h and the highest values were observed with intermediate enzyme levels. All ruminal volatile fatty acids increased linearly with intermediate levels of the fibrolytic enzyme. The in sacco rate of dry matter degradation decreased linearly (P = 0.020) with increasing enzymes. Intermediate levels of xylanase improved rumen kinetic fermentation and degradability. The outcome of this research indicated that the application of xylanase enzyme could improve in vitro GP fermentation of wheat straw.

Editor

Animal Science Journal

Fecha de publicación

10 de octubre de 2014

Tipo de publicación

Artículo

Recurso de información

http://hdl.handle.net/20.500.11799/66030

Idioma

Inglés

Relación

Vol.;86

Audiencia

Estudiantes

Investigadores

Repositorio Orígen

REPOSITORIO INSTITUCIONAL DE LA UAEM

Descargas

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