Título
Macrophage migration inhibitory factor promoter polymorphisms (-794 CATT5 and -173 G
>
C): relationship with mRNA expression and soluble MIF levels in young obese subjects
Autor
Inés Matia García
Lorenzo Salgado Goytia
Samuel García Arellano
Isela Parra Rojas
Nivel de Acceso
Acceso Abierto
Resumen o descripción
This study was supported by the Consejo Nacional de Ciencia y Tecnología, Grant INFR-2014-02/229958. In¿es Matia- García received a fellowship of CONACYT (no. 231182)
We analyzed the relationship of -794 CATT5 and -173 G>C MIF polymorphisms with mRNA and soluble MIF in young obese. A total of 250 young subjects, 150 normal-weight and 100 obese subjects, were recruited in the study. Genotyping of -794 CATT5 and -173 G>C MIF polymorphisms was performed by PCR and PCR-RFLP, respectively. MIF mRNA expression was determined by real-time PCR and serum MIF levels were measured using an ELISA kit. For both MIF promoter polymorphisms, no significant differences in the genotype and allele frequencies between groups were observed. MIF mRNA expression was slightly higher in obese subjects than in normal-weight subjects (1.38-fold), while soluble MIF levels did not show differences between groups. In addition, we found an increase in MIF mRNA expression in carriers of the 6,6 and C/C genotypes and the 6G haplotype of the -794 CATT5 and -173 G>C MIF polymorphisms, although it was not significant. In conclusion, this study found norelationship between obesity and MIF gene promoter polymorphisms with MIF mRNA expression in young obese subjects.
Editor
Hindawi Publishing Corporation
Fecha de publicación
30 de marzo de 2015
Tipo de publicación
Artículo
Recurso de información
Formato
application/pdf
Idioma
Inglés
Audiencia
Público en general
Repositorio Orígen
Repositorio Institucional de Ciencia Abierta de la Universidad Autónoma de Guerrero
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