Título
Visualization and 3d reconstruction of flame cells of Taenia solium (Cestoda).
Autor
Laura Elena Valverde Islas
Esteban Arrangoiz Arechavala
Elio Vega Munguía
Lilia Robert
Rafael Villanueva Olea
Olivia Alicia Reynoso Ducoing
Kaethe Lina Maria Willms Manning
Armando Zepeda
Teresa Imelda Fortoul Vander Goes
Javier Rolando Ambrosio Hernandez
Nivel de Acceso
Acceso Abierto
Identificador alterno
doi: 10.1371/journal.pone.0014754
Materias
Parasitología - ([PLOS ONE (1932-6203) vol. 6(3), 1-11 (2011)]) Cilios - ([PLOS ONE (1932-6203) vol. 6(3), 1-11 (2011)]) Proteínas del citoesqueleto - ([PLOS ONE (1932-6203) vol. 6(3), 1-11 (2011)]) Microscopía electrónica de transmisión - ([PLOS ONE (1932-6203) vol. 6(3), 1-11 (2011)]) Actina - ([PLOS ONE (1932-6203) vol. 6(3), 1-11 (2011)]) Miosina - ([PLOS ONE (1932-6203) vol. 6(3), 1-11 (2011)]) Céstodos - ([PLOS ONE (1932-6203) vol. 6(3), 1-11 (2011)]) Matriz extracelular - ([PLOS ONE (1932-6203) vol. 6(3), 1-11 (2011)]) BIOLOGÍA Y QUÍMICA - (CTI)
Resumen o descripción
BACKGROUND: Flame cells are the terminal cells of protonephridial systems, which are part of the excretory systems of invertebrates. Although the knowledge of their biological role is incomplete, there is a consensus that these cells perform excretion/secretion activities. It has been suggested that the flame cells participate in the maintenance of the osmotic environment that the cestodes require to live inside their hosts. In live Platyhelminthes, by light microscopy, the cells appear beating their flames rapidly and, at the ultrastructural, the cells have a large body enclosing a tuft of cilia. Few studies have been performed to define the localization of the cytoskeletal proteins of these cells, and it is unclear how these proteins are involved in cell function. METHODOLOGY/PRINCIPAL FINDINGS: Parasites of two different developmental stages of T. solium were used: cysticerci recovered from naturally infected pigs and intestinal adults obtained from immunosuppressed and experimentally infected golden hamsters. Hamsters were fed viable cysticerci to recover adult parasites after one month of infection. In the present studies focusing on flame cells of cysticerci tissues was performed. Using several methods such as video, confocal and electron microscopy, in addition to computational analysis for reconstruction and modeling, we have provided a 3D visual rendition of the cytoskeletal architecture of Taenia solium flame cells. CONCLUSIONS/SIGNIFICANCE: We consider that visual representations of cells open a new way for understanding the role of these cells in the excretory systems of Platyhelminths. After reconstruction, the observation of high resolution 3D images allowed for virtual observation of the interior composition of cells. A combination of microscopic images, computational reconstructions and 3D modeling of cells appears to be useful for inferring the cellular dynamics of the flame cell cytoskeleton.
Editor
Public Library of Science
Fecha de publicación
2011
Tipo de publicación
Artículo
Versión de la publicación
Versión publicada
Recurso de información
Formato
application/pdf
Fuente
PLOS ONE (1932-6203) vol. 6(3), 1-11 (2011)
Idioma
Inglés
Relación
https://www.ncbi.nlm.nih.gov/pmc/issues/194635/
Cobertura
US
Repositorio Orígen
Repositorio Institucional de la Facultad de Medicina,UNAM.Departamento de Microbiologia y Parasitologia
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