Título
Maturation Phenotype of Peripheral Blood Monocyte/Macrophage After Stimulation with Lipopolysaccharides in Irritable Bowel Syndrome.
Autor
Oscar Andres Rodriguez Fandiño
Joselin Hernandez Ruiz
Yolanda Lopez Vidal
Luis Charúa
Eustacio Galileo Escobedo Gonzalez
Max Julio Schmulson Wasserman
Nivel de Acceso
Acceso Abierto
Identificador alterno
doi: https://doi.org/10.5056/jnm16137
Materias
Lipopolisacáridos - ([Journal of Neurogastroenterology and Motility (1081-1710) vol. 23(2) 281-288 (2017)]) Monocitos - ([Journal of Neurogastroenterology and Motility (1081-1710) vol. 23(2) 281-288 (2017)]) Receptores de quimiocina CX3CR1 - ([Journal of Neurogastroenterology and Motility (1081-1710) vol. 23(2) 281-288 (2017)]) Síndrome de intestino irritable - ([Journal of Neurogastroenterology and Motility (1081-1710) vol. 23(2) 281-288 (2017)]) BIOLOGÍA Y QUÍMICA - (CTI)
Resumen o descripción
BACKGROUND/AIMS: Abnormal immune regulation and increased intestinal permeability augmenting the passage of bacterial molecules that can activate immune cells, such as monocytes/macrophages, have been reported in irritable bowel syndrome (IBS). The aim was to compare the maturation phenotype of monocytes/macrophages (CD14+) from IBS patients and controls in the presence or absence of Escherichia coli lipopolysaccharides (LPS), in vitro. METHODS: Mononuclear cells were isolated from peripheral blood of 20 Rome II-IBS patients and 19 controls and cultured with or without LPS for 72 hours. The maturation phenotype was examined by flow cytometry as follows: M1-Early (CD11c+CD206-), M2-Advanced (CD11c-CD206+CX3CR1+); expression of membrane markers was reported as mean fluorescence intensity (MFI). The Mann-Whitney test was used and significance was set at P < 0.05. RESULTS: In CD14+ cells, CD11c expression decreased with vs without LPS both in IBS (MFI: 8766.0 ± 730.2 vs 12 920.0 ± 949.2, P < 0.001) and controls (8233.0 ± 613.9 vs 13 750.0 ± 743.3, P < 0.001). M1-Early cells without LPS, showed lower CD11c expression in IBS than controls (MFI: 11 540.0 ± 537.5 vs 13 860.0 ± 893.7, P = 0.040), while both groups showed less CD11c in response to LPS (P < 0.01). Furthermore, the percentage of "Intermediate" (CD11c+CD206+CX3CR1+) cells without LPS, was higher in IBS than controls (IBS = 9.5 ± 1.5% vs C = 4.9 ± 1.4%, P < 0.001). Finally, fractalkine receptor (CX3CR1) expression on M2-Advanced cells was increased when treated with LPS in controls but not in IBS (P < 0.001). CONCLUSIONS: The initial phase of monocyte/macrophage maturation appears to be more advanced in IBS compared to controls. However, the decreased CX3CR1 in patients with IBS, compared to controls, when stimulated with LPS suggests a state of immune activation in IBS.
Editor
Korean Society of Neurogastroenterology and Motility
Fecha de publicación
2017
Tipo de publicación
Artículo
Versión de la publicación
Versión publicada
Recurso de información
Formato
application/pdf
Fuente
Journal of Neurogastroenterology and Motility (1081-1710) vol. 23(2) 281-288 (2017)
Idioma
Inglés
Relación
http://www.jnmjournal.org/journal/list.html?pn=vol
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TG=vol
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s_v=23
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s_n=2
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year=2017
Cobertura
KR
Repositorio Orígen
Repositorio Institucional de la Facultad de Medicina,UNAM.Departamento de Microbiologia y Parasitologia
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