Título
2D-immunoblotting analysis of Sporothrix schenckii cell wall.
Autor
Estela Ruiz Baca
Hector Manuel Mora Montes
Everardo Lopez Romero
Concepcion Toriello Najera
Virgilio Mojica Marin
Norma Urtiz Estrada
Nivel de Acceso
Acceso Abierto
Identificador alterno
doi: 10.1590/S0074-02762011000200021
Materias
Micología - ([Memórias do Instituto Oswaldo Cruz (0074-0276) vol. 106(2), 248-50 (2011)]) Sporothrix schenckii - ([Memórias do Instituto Oswaldo Cruz (0074-0276) vol. 106(2), 248-50 (2011)]) Pared celular - ([Memórias do Instituto Oswaldo Cruz (0074-0276) vol. 106(2), 248-50 (2011)]) Glicoproteínas - ([Memórias do Instituto Oswaldo Cruz (0074-0276) vol. 106(2), 248-50 (2011)]) BIOLOGÍA Y QUÍMICA - (CTI)
Resumen o descripción
We utilized two-dimensional gel electrophoresis and immunoblotting (2D-immunoblotting) with anti-Sporothrix schenckii antibodies to identify antigenic proteins in cell wall preparations obtained from the mycelial and yeast-like morphologies of the fungus. Results showed that a 70-kDa glycoprotein (Gp70) was the major antigen detected in the cell wall of both morphologies and that a 60-kDa glycoprotein was present only in yeast-like cells. In addition to the Gp70, the wall from filament cells showed four proteins with molecular weights of 48, 55, 66 and 67 kDa, some of which exhibited several isoforms. To our knowledge, this is the first 2D-immunoblotting analysis of the S. schenckii cell wall.
Editor
Fundacao Oswaldo Cruz
Fecha de publicación
2011
Tipo de publicación
Artículo
Versión de la publicación
Versión publicada
Recurso de información
Formato
application/pdf
Fuente
Memórias do Instituto Oswaldo Cruz (0074-0276) vol. 106(2), 248-50 (2011)
Idioma
Inglés
Relación
https://memorias.ioc.fiocruz.br/content/past-issues/past/16
Cobertura
BR
Repositorio Orígen
Repositorio Institucional de la Facultad de Medicina,UNAM.Departamento de Microbiologia y Parasitologia
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