Autor: Carlos Eliud Angulo Valadez

Debaryomyces hansenii: una nueva levadura probiótica

Debaryomyces hansenii: A new probiotic yeast

MIRIAM GORETTY ANGULO VILLAVICENCIO MARTHA CANDELARIA REYES BECERRIL Carlos Eliud Angulo Valadez (2022)

"Los probióticos son bichos diminutos cuyo consumo genera efectos benéficos en la salud. De hecho, la palabra probiótico significa 'pro-vida'. Algunas levaduras, como la utilizada para hacer pan o cerveza, pueden ser probióticos para animales y humanos. Desde hace algunos años, los estudios con la levadura Debaryomyces hansenii han demostrado que ésta es un probiótico. Se ha demostrado que, administrada por vía oral, esta levadura tiene efectos estimulantes del sistema de defensa y beneficios para la función digestiva de animales terrestres y acuáticos. Por lo anterior, se describen los hallazgos que revelan que D. hansenii es un probiótico para animales y posiblemente para humanos."

"Probiotics are tiny bugs that when consumed have beneficial effects on health. In fact, the word probiotic means pro-life. Some yeasts, such as that used to make bread or beer, can be probiotics for animals and humans. For some years, studies with the yeast Debaryomyces hansenii indicate that it is a probiotic.

This yeast administered orally has been shown to have stimulating effects on the defense system and beneficial effects on the digestive function of terrestrial and aquatic animals. Therefore, we will try to describe the findings of D. hansenii that indicate that it is a probiotic for animals and possibly also for humans."

Artículo

probióticos, bienestar, microbios, enfermedad, supervivencia probiotics, wellbeing, microbes, disease, survival BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA MICROBIOLOGÍA MICOLOGÍA (LEVADURAS) MICOLOGÍA (LEVADURAS)

De Novo assembly and annotation of the Pacific calico scallop (Argopecten ventricosus) transcriptome for immune-related gene discovery

RUTH ESCAMILLA MONTES GENARO DIARTE PLATA GABRIELA BERENICE MENDOZA MALDONADO Aarón Barraza Celis Carlos Eliud Angulo Valadez Seyed Hossein Hoseinifar Jesús Arturo Fierro Coronado ANTONIO LUNA GONZALEZ (2022)

"Invertebrates' immune defense mechanisms play a critical role in pathogen recognition and elimination. De novo assembly and annotation of the Argopecten ventricosus transcriptome were performed for the immune-related gene identification. Scallops (height: 4.4 cm) were challenged with inactivated Vibrio parahaemolyticus IPNGS16. The RNA from different tissues was pooled for a single cDNA library construction sequenced by NextSeq 500 platform 2×75 paired‐end chemistry. Before de novo assembling with Trinity, reads were analyzed with FastQC, Trimmomatic, and Prinseq. Assembled sequences were analyzed by CD-HIT-EST and TransDecoder. The corresponding annotation was performed against NCBI-nr, RefSeq protein, and KAAS (KEGG) databases. The Trinity assembly yielded 107,516 contigs. TransDecoder yielded 25,285 sequences as CDSs of which, 16,123 were annotated against the NCBI-nr protein, most of them scored with Crassostrea gigas data. Gene ontology mapped sequences (15,262) were classified in molecular functions (~13,000), cellular components (~11,000), and biological processes (~13,000). The KAAS analysis showed biological categories for metabolism (13%), cellular processes (12%), genetic information processing (10%), organismal systems (19%), environmental information (13%), and human diseases (33 %). Within the organismal systems, 467 immune-related genes (KO) were identified. Sixty-four immune-related genes were annotated/blasted against the NCBI-nr and RefSeq protein databases. An RT-qPCR was performed to analyze the expression level of immune-related genes obtained in the transcriptome analysis in scallops (height 4.5 cm) treated with probiotic bacilli added to culture water. Bacilli significantly increased the expression of the HSP70 and PGRP genes. The gene transcripts analysis of A. ventricosus will better understand its immune response against pathogens in culture systems."

Artículo

Argopecten ventricosus, calico scallop, transcriptome, bivalve, immune genes BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA BIOLOGÍA ANIMAL (ZOOLOGÍA) INMUNOLOGÍA INMUNOLOGÍA