Construcción de plásmidos del virus de Chikungunya, de replicación y ayuda para la transfección de células de mamíferos

Construction of a Chikungunya Virus, Replicon, and Helper Plasmids for Transfection of Mammalian Cells

Mayra Colunga-Saucedo (2023)

The genome of Alphaviruses can be modified to produce self-replicating RNAs and virus-like particles, which are useful virological tools. In this work, we generated three plasmids for

the transfection of mammalian cells: an infectious clone of Chikungunya virus (CHIKV), one that

codes for the structural proteins (helper plasmid), and another one that codes nonstructural proteins

(replicon plasmid). All of these plasmids contain a reporter gene (mKate2). The reporter gene in

the replicon RNA and the infectious clone are synthesized from subgenomic RNA. Co-transfection

with the helper and replicon plasmids has biotechnological/biomedical applications because they

allow for the delivery of self-replicating RNA for the transient expression of one or more genes to the

target cells.

Tesis de doctorado

MEDICINA Y CIENCIAS DE LA SALUD