Título
Phylogenetic relationships and acaricidal effects of beauveria bassiana obtained from cattle farm soils against rhipicephalus microplus
Autor
AGUSTIN FERNANDEZ SALAS
MIGUEL ANGEL ALONSO DIAZ
ROGELIO ALEJANDRO ALONSO MORALES
ROBERTO LEZAMA GUTIERREZ
JOSE ANTONIO CERVANTES CHAVEZ
Nivel de Acceso
Acceso Abierto
Identificador alterno
isni/0000 0004 0428 7635
Materias
Resumen o descripción
The objectives of the present study were to isolate Beauveria bassiana strains from cattle farm soils, analyze the phylogenetic relationships among the fungal strains isolated from these soils, and determine the acaricidal effect of B. bassiana isolates on engorged Rhipicephalus microplus tick strains resistant or susceptible to chemical acaricides. Six strains of B. bassiana were obtained and isolated from cattle farm soils in the Mexican tropics using the Galleria bait method, and their acaricidal effect was assessed against 2 populations of R. microplus (“Media Joya” chemical acaricide-resistant strain or “CLAR” chemical acaricide-susceptible strain) using the adult immersion test. The BbV03 strain produced 86.7% and 60% mortality in resistant and susceptible ticks on day 20, respectively, whereas the BbV04 strain produced 66.7% and 53.5% mortality in resistant and susceptible ticks on day 20, respectively. The BbV03 and BbV04 strains reduced egg laying on both R. microplus populations. There was no statistical difference in the acaricidal effect of B. bassiana strains among chemical acaricide-susceptible or -resistant R. microplus populations (P > 0.05). The BbV03 strain was the most virulent against R. microplus with an LC50 of 2 × 107 and LC99 of 7 × 108 conidia/ml. We found that the 6 B. bassiana isolated clustered in the same clade with other previously reported B. bassiana strains (from GenBank) but were separated into 3 different sub-clades. This study shows that some B. bassiana strains are a promising coadjuvant alternative for biological tick control, including tick populations that are resistant to chemical acaricides. Beauveria bassiana is present in the pastures of tropic cattle farms, and there are genetic variations between B. bassiana strains living in this ecosystem that might play an important role in the natural control of R. microplus in cattle farm paddocks.
Editor
BioOne
Fecha de publicación
1 de junio de 2018
Tipo de publicación
Artículo
Recurso de información
Idioma
Inglés
Repositorio Orígen
Repositorio institucional de la Universidad de Colima
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