Título

Transcriptome mining provides insights into cell wall metabolism and fiber lignification in Agave tequilana Weber

Autor

Luis Fernando Maceda Lopez

ELSA BEATRIZ GONGORA CASTILLO

Enrique Ibarra-Laclette

DALIA C. MORAN VELAZQUEZ

AMARANTA GIRON RAMIREZ

Matthieu Bourdon

José Luis Villalpando Aguilar

Gabriela Chavez-Calvillo Toomer

John Tang

Parastoo Azadi

Jorge Manuel Santamaría Fernández

Itzel López-Rosas

Mercedes G Lopez

June Simpson

FULGENCIO ALATORRE COBOS

Nivel de Acceso

Acceso Abierto

Licencia

http://creativecommons.org/licenses/by-nc-nd/4.0

Referencia de datos

datasetDOI/https://doi.org/10.3390/plants11111496

Materias

AGAVE - (AUTORES) CELL WALLS - (AUTORES) LIGNOCELLULOSE - (AUTORES) CAD PROTEIN - (AUTORES) CESA PROTEIN - (AUTORES) SCLERENCHYMA - (AUTORES) BIOLOGÍA Y QUÍMICA - (CTI) CIENCIAS DE LA VIDA - (CTI) GENÉTICA - (CTI) GENÉTICA MOLECULAR DE PLANTAS - (CTI) GENÉTICA MOLECULAR DE PLANTAS - (CTI)

Resumen o descripción

Resilience of growing in arid and semiarid regions and a high capacity of accumulating sugar-rich biomass with low lignin percentages have placed Agave species as an emerging bioen-ergy crop. Although transcriptome sequencing of fiber-producing agave species has been explored, molecular bases that control wall cell biogenesis and metabolism in agave species are still poorly understood. Here, through RNAseq data mining, we reconstructed the cellulose biosynthesis pathway and the phenylpropanoid route producing lignin monomers in A. tequilana, and evaluated their expression patterns in silico and experimentally. Most of the orthologs retrieved showed differential expression levels when they were analyzed in different tissues with contrasting cellulose and lignin accumulation. Phylogenetic and structural motif analyses of putative CESA and CAD proteins allowed to identify those potentially involved with secondary cell wall formation. RT-qPCR assays revealed enhanced expression levels of AtqCAD5 and AtqCESA7 in parenchyma cells associated with extraxylary fibers, suggesting a mechanism of formation of sclerenchyma fibers in Agave similar to that reported for xylem cells in model eudicots. Overall, our results provide a framework for un-derstanding molecular bases underlying cell wall biogenesis in Agave species studying mechanisms involving in leaf fiber development in monocots. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.

Fecha de publicación

2022

Tipo de publicación

Artículo

Versión de la publicación

Versión publicada

Recurso de información

http://cicy.repositorioinstitucional.mx/jspui/handle/1003/2856

Formato

application/pdf

Fuente

Plants, 11(11), 1496, 2022.

Idioma

Inglés

Sugerencia de citación

Maceda-López, L.F.; Góngora-Castillo, E.B.; Ibarra-Laclette, E.; Morán-Velázquez, D.C.; Girón Ramírez, A.; Bourdon, M.; Villalpando-Aguilar, J.L.; Toomer, G.; Tang, J.Z.; Azadi, P.; et al. Transcriptome mining provides insights into cell wall metabolism and fiber lignification in Agave tequilana Weber. Plants 2022, 11, 1496. https://doi.org/10.3390/plants11111496

Repositorio Orígen

Repositorio Institucional CICY

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