Title
Transcriptome mining provides insights into cell wall metabolism and fiber lignification in Agave tequilana Weber
Author
Luis Fernando Maceda Lopez
ELSA BEATRIZ GONGORA CASTILLO
Enrique Ibarra-Laclette
DALIA C. MORAN VELAZQUEZ
AMARANTA GIRON RAMIREZ
Matthieu Bourdon
José Luis Villalpando Aguilar
Gabriela Chavez-Calvillo Toomer
John Tang
Parastoo Azadi
Jorge Manuel Santamaría Fernández
Itzel López-Rosas
Mercedes G Lopez
June Simpson
FULGENCIO ALATORRE COBOS
Access level
Open Access
Dataset reference
datasetDOI/https://doi.org/10.3390/plants11111496
Subjects
Summary or description
Resilience of growing in arid and semiarid regions and a high capacity of accumulating sugar-rich biomass with low lignin percentages have placed Agave species as an emerging bioen-ergy crop. Although transcriptome sequencing of fiber-producing agave species has been explored, molecular bases that control wall cell biogenesis and metabolism in agave species are still poorly understood. Here, through RNAseq data mining, we reconstructed the cellulose biosynthesis pathway and the phenylpropanoid route producing lignin monomers in A. tequilana, and evaluated their expression patterns in silico and experimentally. Most of the orthologs retrieved showed differential expression levels when they were analyzed in different tissues with contrasting cellulose and lignin accumulation. Phylogenetic and structural motif analyses of putative CESA and CAD proteins allowed to identify those potentially involved with secondary cell wall formation. RT-qPCR assays revealed enhanced expression levels of AtqCAD5 and AtqCESA7 in parenchyma cells associated with extraxylary fibers, suggesting a mechanism of formation of sclerenchyma fibers in Agave similar to that reported for xylem cells in model eudicots. Overall, our results provide a framework for un-derstanding molecular bases underlying cell wall biogenesis in Agave species studying mechanisms involving in leaf fiber development in monocots. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
Publish date
2022
Publication type
Article
Publication version
Published Version
Information Resource
Format
application/pdf
Source
Plants, 11(11), 1496, 2022.
Language
English
Citation suggestion
Maceda-López, L.F.; Góngora-Castillo, E.B.; Ibarra-Laclette, E.; Morán-Velázquez, D.C.; Girón Ramírez, A.; Bourdon, M.; Villalpando-Aguilar, J.L.; Toomer, G.; Tang, J.Z.; Azadi, P.; et al. Transcriptome mining provides insights into cell wall metabolism and fiber lignification in Agave tequilana Weber. Plants 2022, 11, 1496. https://doi.org/10.3390/plants11111496
Source repository
Repositorio Institucional CICY
Downloads
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