Title

Transcriptome mining provides insights into cell wall metabolism and fiber lignification in Agave tequilana Weber

Author

Luis Fernando Maceda Lopez

ELSA BEATRIZ GONGORA CASTILLO

Enrique Ibarra-Laclette

DALIA C. MORAN VELAZQUEZ

AMARANTA GIRON RAMIREZ

Matthieu Bourdon

José Luis Villalpando Aguilar

Gabriela Chavez-Calvillo Toomer

John Tang

Parastoo Azadi

Jorge Manuel Santamaría Fernández

Itzel López-Rosas

Mercedes G Lopez

June Simpson

FULGENCIO ALATORRE COBOS

Access level

Open Access

Dataset reference

datasetDOI/https://doi.org/10.3390/plants11111496

Summary or description

Resilience of growing in arid and semiarid regions and a high capacity of accumulating sugar-rich biomass with low lignin percentages have placed Agave species as an emerging bioen-ergy crop. Although transcriptome sequencing of fiber-producing agave species has been explored, molecular bases that control wall cell biogenesis and metabolism in agave species are still poorly understood. Here, through RNAseq data mining, we reconstructed the cellulose biosynthesis pathway and the phenylpropanoid route producing lignin monomers in A. tequilana, and evaluated their expression patterns in silico and experimentally. Most of the orthologs retrieved showed differential expression levels when they were analyzed in different tissues with contrasting cellulose and lignin accumulation. Phylogenetic and structural motif analyses of putative CESA and CAD proteins allowed to identify those potentially involved with secondary cell wall formation. RT-qPCR assays revealed enhanced expression levels of AtqCAD5 and AtqCESA7 in parenchyma cells associated with extraxylary fibers, suggesting a mechanism of formation of sclerenchyma fibers in Agave similar to that reported for xylem cells in model eudicots. Overall, our results provide a framework for un-derstanding molecular bases underlying cell wall biogenesis in Agave species studying mechanisms involving in leaf fiber development in monocots. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.

Publish date

2022

Publication type

Article

Publication version

Published Version

Format

application/pdf

Source

Plants, 11(11), 1496, 2022.

Language

English

Citation suggestion

Maceda-López, L.F.; Góngora-Castillo, E.B.; Ibarra-Laclette, E.; Morán-Velázquez, D.C.; Girón Ramírez, A.; Bourdon, M.; Villalpando-Aguilar, J.L.; Toomer, G.; Tang, J.Z.; Azadi, P.; et al. Transcriptome mining provides insights into cell wall metabolism and fiber lignification in Agave tequilana Weber. Plants 2022, 11, 1496. https://doi.org/10.3390/plants11111496

Source repository

Repositorio Institucional CICY

Downloads

31

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