Título

A DNA Microarray-based assay to detect dual infection with two dengue virus serotypes

Autor

Juan Burgueño

Julio G Mendoza-Alvarez

Nivel de Acceso

Acceso Abierto

Licencia

http://creativecommons.org/licenses/by/4.0

Materias

Dengue Virus - (AGROVOC) Humans - (AGROVOC) Aedes - (AGROVOC) Microarrays - (AGROVOC) CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA - (CTI)

Resumen o descripción

Here; we have described and tested a microarray based-method for the screening of dengue virus (denv) serotypes. This dna microarray assay is specific and sensitive and can detect dual infections with two dengue virus serotypes and single-serotype infections. Other methodologies may underestimate samples containing more than one serotype. This technology can be used to discriminate between the four denv serotypes. Single-stranded dna targets were covalently attached to glass slides and hybridised with specific labelled probes. Denv isolates and dengue samples were used to evaluate microarray performance. Our results demonstrate that the probes hybridized specifically to denv serotypes; with no detection of unspecific signals. This finding provides evidence that specific probes can effectively identify single and double infections in denv samples

Fecha de publicación

2014

Tipo de publicación

Artículo

Recurso de información

https://hdl.handle.net/10883/19772

Formato

application/pdf

Idioma

Inglés

Audiencia

Investigadores

Repositorio Orígen

Repositorio Institucional de Publicaciones Multimedia del CIMMYT

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