Título

Phylogenetic diversity of methyl-coenzyme M reductase (mcrA) gene and methanogenesis from trimethylamine in hypersaline environments

Autor

JOSE QUINATZIN GARCIA MALDONADO

MARIA DE LOURDES BERENICE CELIS GARCIA

ALEJANDRO LOPEZ CORTES

Nivel de Acceso

Acceso Abierto

Licencia

http://creativecommons.org/licenses/by-nc-nd/4.0

Identificador alterno

doi: https://doi.org/10.2436/20.1501.01.155

Materias

Methanosarcinaceae - (AUTOR) Hypersaline environments - (AUTOR) Microbial mats - (AUTOR) Trimethylamine - (AUTOR) Gene mcrA - (AUTOR) CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA - (CTI)

Resumen o descripción

"Methanogens have been reported in complex microbial communities from hypersaline environments, but little is known about their phylogenetic diversity. In this work, methane concentrations in environmental gas samples were determined while methane production rates were measured in microcosm experiments with competitive and non-competitive substrates. In addition, the phylogenetic diversity of methanogens in microbial mats from two geographical locations was analyzed: the well studied Guerrero Negro hypersaline ecosystem, and a site not previously investigated, namely Laguna San Ignacio, Baja California Sur, Mexico. Methanogenesis in these microbial mats was suspected based on the detection of methane (in the range of 0.00086 to 3.204 %) in environmental gas samples. Microcosm experiments confirmed methane production by the mats and demonstrated that it was promoted only by non-competitive substrates (trimethylamine and methanol), suggesting that methylotrophy is the main characteristic process by which these hypersaline microbial mats produce methane. Phylogenetic analysis of amino acid sequences of the methyl coenzyme-M reductase (mcrA) gene from natural and manipulated samples revealed various methylotrophic methanogens belonging exclusively to the family Methanosarcinaceae. Moderately halophilic microorganisms of the genus Methanohalophilus were predominant (>60 % of mcrA sequences retrieved). Slightly halophilic and marine microorganisms of the genera Methanococcoides and Methanolobus, respectively, were also identified, but in lower abundances."

Fecha de publicación

2012

Tipo de publicación

Artículo

Versión de la publicación

Versión publicada

Recurso de información

http://ipicyt.repositorioinstitucional.mx/jspui/handle/1010/1244

Formato

application/pdf

Idioma

Inglés

Relación

&

López-Cortés A.. (2012). Phylogenetic diversity of methyl-coenzyme M reductase (mcrA) gene and methanogenesis from trimethylamine in hypersaline environments.. International Microbiology, 15, 33-41 https://doi.org/10.2436/20.1501.01.155

Audiencia

Público en general

Sugerencia de citación

García-Maldonado JQ, Bebout BM, Celis LB

Repositorio Orígen

Repositorio IPICYT

Descargas

400