Título
Lipofection improves gene targeting efficiency in E14 TG2a mouse embryonic stem cells
Autor
SANDRA MARICRUZ LOPEZ HEYDECK
Marcos Cajero Juárez
ROGELIO ALEJANDRO ALONSO MORALES
JOSE SIMON MARTINEZ CASTAÑEDA
Jose Francisco Robles González
ALBERTO BARBABOSA PLIEGO
JUAN CARLOS VAZQUEZ CHAGOYAN
Nivel de Acceso
true
Acceso Abierto
Materias
veterinaria - ([Veterinaria México (México) Num.1 Vol.40]) gene targeting - ([Veterinaria México (México) Num.1 Vol.40]) homologous recombination - ([Veterinaria México (México) Num.1 Vol.40]) mouse embryonic stem cells - ([Veterinaria México (México) Num.1 Vol.40]) transfection - ([Veterinaria México (México) Num.1 Vol.40]) electroporation - ([Veterinaria México (México) Num.1 Vol.40]) lipofection - ([Veterinaria México (México) Num.1 Vol.40]) CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA - (CTI)
Resumen o descripción
Electroporation has been the method of election for transfection of murine embryonic stem cells for over 15 years; however, it is a time consuming protocol because it requires large amounts of DNA and cells, as well as expensive and delicate equipment. Lipofection is a transfection method that requires lower amounts of cells and DNA than electroporation, and has proven to be effi cient in a large number of cell lines. It has been shown that after lipofection, mouse embryonic stem cells remain pluripotent, capable of forming germ line chimeras and can be transfected with greater effi ciency than with electroporation; however, gene targeting of mouse embryonic stem cells by lipofection has not been reported. The objective of this work was to fi nd out if lipofection can be used as effi ciently as electroporation for regular gene targeting protocols. This context compares gene targeting effi ciency between these techniques in mouse embryonic stem cells E14TG2a, using a gene replacement type vector. No differences were found in gene targeting effi ciency between groups; however, lipofection was three times more effi cient than electroporation in transfection effi ciency, which makes lipofection a less expensive alternative method to produce gene targeting in mouse embryonic stem cells.
Editor
Universidad Nacional Autónoma de México
Fecha de publicación
2009
Tipo de publicación
Artículo
Recurso de información
Formato
application/application/pdf
Fuente
Veterinaria México (México) Num.1 Vol.40
Idioma
Inglés
Relación
http://www.redalyc.org/revista.oa?id=423
Audiencia
Estudiantes
Investigadores
Repositorio Orígen
REPOSITORIO INSTITUCIONAL DE LA UAEM
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