Title

Lipofection improves gene targeting efficiency in E14 TG2a mouse embryonic stem cells

Author

SANDRA MARICRUZ LOPEZ HEYDECK

Marcos Cajero Juárez

ROGELIO ALEJANDRO ALONSO MORALES

JOSE SIMON MARTINEZ CASTAÑEDA

Jose Francisco Robles González

ALBERTO BARBABOSA PLIEGO

JUAN CARLOS VAZQUEZ CHAGOYAN

Access level

true

Open Access

Summary or description

Electroporation has been the method of election for transfection of murine embryonic stem cells for over 15 years; however, it is a time consuming protocol because it requires large amounts of DNA and cells, as well as expensive and delicate equipment. Lipofection is a transfection method that requires lower amounts of cells and DNA than electroporation, and has proven to be effi cient in a large number of cell lines. It has been shown that after lipofection, mouse embryonic stem cells remain pluripotent, capable of forming germ line chimeras and can be transfected with greater effi ciency than with electroporation; however, gene targeting of mouse embryonic stem cells by lipofection has not been reported. The objective of this work was to fi nd out if lipofection can be used as effi ciently as electroporation for regular gene targeting protocols. This context compares gene targeting effi ciency between these techniques in mouse embryonic stem cells E14TG2a, using a gene replacement type vector. No differences were found in gene targeting effi ciency between groups; however, lipofection was three times more effi cient than electroporation in transfection effi ciency, which makes lipofection a less expensive alternative method to produce gene targeting in mouse embryonic stem cells.

Publisher

Universidad Nacional Autónoma de México

Publish date

2009

Publication type

Article

Format

application/application/pdf

Source

Veterinaria México (México) Num.1 Vol.40

Language

English

Relation

http://www.redalyc.org/revista.oa?id=423

Audience

Students

Researchers

Source repository

REPOSITORIO INSTITUCIONAL DE LA UAEM

Downloads

0

Comments



You need to sign in or sign up to comment.