Título

In vitro larvicidal effect of a hydroalcoholic extract from Acacia cochliacantha leaf against ruminant parasitic nematodes

Autor

AGUSTIN OLMEDO JUAREZ

ALEJANDRO ZAMILPA ALVAREZ

ROLANDO ROJO RUBIO

PEDRO MENDOZA DE GIVES

MARIA EUGENIA LOPEZ ARELLANO

JAVIER ARECE GARCIA

ELKE VON SON DE FERNEX

Nivel de Acceso

Acceso Abierto

Licencia

http://creativecommons.org/licenses/by-nc-nd/4.0

Materias

haemonchus - ([1573-7446]) cooperia - ([1573-7446]) tannins - ([1573-7446]) flavonoids - ([1573-7446]) nematodes - ([1573-7446]) acacia cochliacantha - ([1573-7446]) CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA - (CTI)

Resumen o descripción

Larvicidal effect was evaluated using acacia cochliacantha leaf

The aim of this study was to evaluate the in vitro lethal effect of a hydroalcoholic extract (HAE) from Acacia cochliacantha leaf against three gastrointestinal nematodes species (Haemonchus contortus, H. placei and Cooperiapunctata) of domestic ruminants. The HAE was assessed using five concentrations: 100, 125, 175, 150 and 200 mg/ml; 0.5% Ivermectin was used as a positive control and distilled water, as negative control. The data were normalized using the square root and analysed with a completely randomized design through ANOVA analysis using the general linealmodel (GLM) of the SAS program. The HAE tannin conten was determined through spectrophotometry (UV-visible) and the other major phenols, were identified by chromatographic processes. The results showed an in vitro larvicidal activity of the HAE against the three assessed nematode species with all assessed concentrations. A clear HAE increased concentration dependence effect was observed. The highest activity of the HAE was obtained at the highest concentration (close to 100%, P < 0.05). This result was similar to the one obtained with Ivermectin. On the other hand, the chemical analysis of HAE showed the presence of tannins, caffeoyls and coumaroyl derivates and quercetin as the main compounds. The results suggest that the HAE from this plant species pos- sess in vitro anthelmintic properties. The identified com- pounds in this study would good candidates for further in vivo researches.

This study received financial support from CONACYT-Mexico (Project number 251244), INIFAP (Project number 9454133183, through the Researcher Retaining Program).

Editor

SPRINGER

Fecha de publicación

15 de marzo de 2017 15 de marzo de 2017

Tipo de publicación

Artículo

Recurso de información

http://hdl.handle.net/20.500.11799/68889

Fuente

1573-7446

Idioma

Inglés

Audiencia

Estudiantes

Investigadores

Repositorio Orígen

REPOSITORIO INSTITUCIONAL DE LA UAEM

Descargas

569