Author: RAMIRO BAEZA JIMENEZ
In order to improve the enzymatic production of biodiesel, the reuse of an immobilized preparation of Candida antarctica lipase B (CALB) was assayed under di ff erent conditions. The improvement included: (1) reuse of CALB in the presence of ethanol (EtOH), (2) reuse of CALB in the presence of methanol (MeOH), (3) blended alcohols at di ff erent molar ratios (0:100, 20:80, 40:60, 50:50, 60:40, 80:20 and 100:0, %EtOH:%MeOH), and employing second generation raw materials: waste cooking oil (WCO) and castor oil (CO). When the first strategy was carried out the FAEE (fatty acid ethyl ester) content achieved was 96% in a first cycle but after four cycles the percentage decreased to 32%. For the second strategy the FAME (fatty acid methyl ester) content attained was 63% in a first cycle but after four cycles the percentage was only 13.5%. When the blended alcohols were tested, the negative e ff ect of MeOH was evident for higher concentrations regardless the type of oil used.
with the addition of molecular sieves (MS) at different addition times (3, 9, 12 and 15 h) and loads (2.5, 5, 10 and 15%, based on the total weight of substrates), the enrichment of GLA from evening primrose oil (EPO) and 1- butanol (BtOH) was improved via Candida rugosa lipase-catalysed esterification reactions. Secondly, the GLAenriched fraction was separated by thin-layer chromatography (TLC) to be further set to react during the third step in the presence of glycerol and Candida antarctica fraction B (CALB), under different enzyme loadings (5, 10, 15 and 20%, based on the total weight of substrates), temperatures (30, 40, 50 and 60 ◦C) and substrates molar ratios (1:1, 2:1, 3:1 and 4:1, GLA:glycerol). 60% of STAG containing 49 wt% of GLA were produced by using 15% of CALB at 60 ◦C and a 3:1 molar ratio.
Conacyt: Beca no. 328716 UAEMex: Proyecto 3866/2015/PIC