Whole-genome comparison between reference sequences and oyster Vibrio vulnificus C-genotype strains

CARLOS ABRAHAM GUERRERO RUIZ (2019, [Artículo])

Whole-genome sequences of Vibrio vulnificus clinical genotype (C-genotype) from the CICESE Culture Collection, isolated from oysters, were compared with reference sequences of CMCP6 and YJ016 V. vulnificus C-genotype strains of clinical origin. The RAST web server estimated the whole genome to be ~4.8 Mb in CICESE strain 316 and ~4.7 Mb in CICESE strain 325. No plasmids were detected in the CICESE strains. Based on a phylogenetic tree that was constructed with the whole-genome results, we observed high similarity between the reference sequences and oyster C-genotype isolates and a sharp contrast with environmental genotype (E-genotype) reference sequences, indicating that the differences between the C- and E-genotypes do not necessarily correspond to their isolation origin. The CICESE strains share 3488 genes (63.2%) with the YJ016 strain and 3500 genes (63.9%) with the CMCP6 strain. A total of 237 pathogenicity associated genes were selected from reference clinical strains, where—92 genes were from CMCP6, 126 genes from YJ016, and 19 from MO6-24/ O; the presence or absence of these genes was recorded for the CICESE strains. Of the 92 genes that were selected for CMCP6, 67 were present in both CICESE strains, as were as 86 of the 126 YJ016 genes and 13 of the 19 MO6-24/O genes. The detection of elements that are related to virulence in CICESE strains—such as the RTX gene cluster, vvhA and vvpE, the type IV pili cluster, the XII genomic island, and the viuB genes, suggests that environmental isolates with the C-genotype, have significant potential for infection. © 2019 Guerrero et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Article, bacterial gene, bacterial strain, bacterial virulence, comparative study, controlled study, gene cluster, gene identification, genomic island, genotype, nonhuman, phylogenetic tree, sequence analysis, strain identification, Vibrio vulnificus BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA GENÉTICA GENÉTICA

Genetic analysis of Vibrio parahaemolyticus O3:K6 strains that have been isolated in Mexico since 1998

CARLOS ABRAHAM GUERRERO RUIZ (2017, [Artículo])

Vibrio parahaemolyticus is an important human pathogen that has been isolated worldwide from clinical cases, most of which have been associated with seafood consumption. Environmental and clinical toxigenic strains of V. parahaemolyticus that were isolated in Mexico from 1998 to 2012, including those from the only outbreak that has been reported in this country, were characterized genetically to assess the presence of the O3:K6 pandemic clone, and their genetic relationship to strains that are related to the pandemic clonal complex (CC3). Pathogenic tdh+ and tdh+/trh+ strains were analyzed by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). Also, the entire genome of a Mexican O3:K6 strain was sequenced. Most of the strains were tdh/ORF8-positive and corresponded to the O3:K6 serotype. By PFGE and MLST, there was very close genetic relationship between ORF8/O3:K6 strains, and very high genetic diversities from non-pandemic strains. The genetic relationship is very close among O3:K6 strains that were isolated in Mexico and sequences that were available for strains in the CC3, based on the PubMLST database. The whole-genome sequence of CICESE-170 strain had high similarity with that of the reference RIMD 2210633 strain, and harbored 7 pathogenicity islands, including the 4 that denote O3:K6 pandemic strains. These results indicate that pandemic strains that have been isolated in Mexico show very close genetic relationship among them and with those isolated worldwide. © 2017 Guerrero et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Article, bacterial strain, biofouling, controlled study, Crassostrea, food intake, gene sequence, genetic analysis, genetic variability, Japan, Mexican, Mexico, molecular phylogeny, nonhuman, pandemic, pathogenicity island, sea food, serotyping, toxi BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA GENÉTICA GENÉTICA

The Banana MaWRKY18, MaWRKY45, MaWRKY60 and MaWRKY70 Genes Encode Functional Transcription Factors and Display Differential Expression in Response to Defense Phytohormones

SERGIO GARCIA LAYNES VIRGINIA AURORA HERRERA VALENCIA Lilia Guadalupe Tamayo Torres VERONICA LIMONES BRIONES FELIPE ALONSO BARREDO POOL FRAY MARTIN BAAS ESPINOLA Ángel Gabriel Alpuche Solís CARLOS ALBERTO PUCH HAU SANTY PERAZA ECHEVERRIA (2022, [Artículo])

"WRKY transcription factors (TFs) play key roles in plant defense responses through phytohormone signaling pathways. However, their functions in tropical fruit crops, especially in banana, remain largely unknown. Several WRKY genes from the model plants rice (OsWRKY45) and Arabidopsis (AtWRKY18, AtWRKY60, AtWRKY70) have shown to be attractive TFs for engineering disease resistance. In this study, we isolated four banana cDNAs (MaWRKY18, MaWRKY45, MaWRKY60, and MaWRKY70) with homology to these rice and Arabidopsis WRKY genes. The MaWRKY cDNAs were isolated from the wild banana Musa acuminata ssp. malaccensis, which is resistant to several diseases of this crop and is a progenitor of most banana cultivars. The deduced amino acid sequences of the four MaWRKY cDNAs revealed the presence of the conserved WRKY domain of ~60 amino acids and a zinc-finger motif at the N-terminus. Based on the number of WRKY repeats and the structure of the zinc-finger motif, MaWRKY18 and MaWRKY60 belong to group II of WRKY TFs, while MaWRKY45 and MaWRKY70 are members of group III. Their corresponding proteins were located in the nuclei of onion epidermal cells and were shown to be functional TFs in yeast cells. Moreover, expression analyses revealed that the majority of these MaWRKY genes were upregulated by salicylic acid (SA) or methyl jasmonate (MeJA) phytohormones, although the expression levels were relatively higher with MeJA treatment. The fact that most of these banana WRKY genes were upregulated by SA or MeJA, which are involved in systemic acquired resistance (SAR) or induced systemic resistance (ISR), respectively, make them interesting candidates for bioengineering broad-spectrum resistance in this crop."

Banana Transcription factor WRKY Defense phytohormones Salicylic acid Methyl jasmonate SAR ISR Broad-spectrum resistance BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA GENÉTICA GENÉTICA

Evaluación del acondicionamiento reproductivo del ostión japonés (Magallana gigas) en dos sistemas de recirculación con prevalencia de polidóridos mediante la expresión de genes

Evaluation of Japanese oyster (Magallana gigas) broodstock conditioning in two recirculating systems with the prevalence of Polidorids through gene expression

Adrián Andrés Morales Guadarrama (2024, [Tesis de maestría])

El ostión Magallana gigas es ampliamente cultivado a nivel mundial. En Baja California, México, los laboratorios de reproducción deben acondicionar ostiones para su maduración y producción de gametos, y abastecer de semilla a los productores. En nuestro laboratorio, Sistemas de Recirculación Acuícola (SRA), con control del sistema CO2-Carbonatos (SRA-R) o sin control (SRA-C), han permitido madurar ostiones M. gigas y M. sikamea. Recientemente, los ostiones M. gigas acondicionados en nuestros SRA no maduraron, y algunos presentaron prevalencia de polidóridos (PP), poliquetos parásitos excavadores de concha. Para comprender la condición de los ostiones, evaluamos el efecto del SRA y de la PP sobre la expresión relativa de ocho genes asociados a biomineralización (VpATP y Tyr), inmunidad innata (P38, PGRP-L y TLR2) y reproducción (GnRH-RI, Vasa-like y SP1b) de M. gigas en dos etapas del acondicionamiento, 18 °C y 24 °C. La PP se determinó por la presencia de ampollas en la concha. Mediante RT-qPCR se determinó la expresión de VpATP, Tyr, P38, PGRP-L y TLR2 en el manto, y de GnRH-RI, Vasa-like y SP1b en la gónada. La expresión relativa se evaluó con un enfoque estadístico basado en un análisis Bayesiano de dos vías y comparaciones múltiples, p-valor significativo < 0.05 y corrección de Bonferroni. En 18 °C, la expresión de VpATP, Tyr, TLR2 y P38 fue mayor en ostiones con PP (CPP) que sin PP (SPP). En contraste, la expresión de GnRH-RI, Vasa-like y SP1b fue menor CPP que SPP. Dentro del SRA-C, en los ostiones CPP hubo mayor expresión de Tyr y menor expresión de Vasa-like y SP1b, respecto a los ostiones SPP. Esto sugiere que la PP induce la reparación de la concha y las respuestas inmune e inflamatoria en el manto mientras que en la gónada reduce el desarrollo de las células germinales. En 24 °C, en el SRA-R hubo menor expresión de SP1b respecto del SRA-C y sugiere menor división celular en la gónada. En conclusión, el SRA-R y la PP afectaron el balance energético del ostión japonés, limitando la energía y reflejando menor esfuerzo reproductivo en los ostiones del SRA-R al final del acondicionamiento reproductivo.

The Japanese oyster (Magallana gigas) is highly cultured worldwide. In Baja California, Mexico, the reproduction laboratories must condition oysters to maturity and have gametes to supply oyster seeds to producers. In our laboratory, recirculating aquaculture systems (RAS), with control of the CO2-Carbonate system (RAS-R) and without this control (RAS-C), have allowed oysters M. gigas and M. sikamea to mature. Recently, the M. gigas oysters conditioned in our RAS did not mature, and some have the prevalence of Polidorids (PP), shell-boring polychaete parasites. To understand the oysters' condition, we evaluated the effect of RAS and the PP on the relative expression of eight genes associated with biomineralization (VpATP and Tyr), innate immunity (P38, PGRP-L, and TLR2), and reproduction (GnRH-RI, Vasa-like, and SP1b) of M. gigas at two phases of broodstock conditioning, 18 °C and 24 °C. The PP was determined by mud blisters at the inner oyster shell. RT-qPCR determined the expression of VpATP, Tyr, p38, PGRP-L, and TLR2 in the mantle tissue and GnRH-RI, Vasa-like, and SP1b in the gonad tissue. The relative gene expression was evaluated by a Bayesian statistics frame based on a two-way and multiple comparison analysis, with significant p-value < 0.05 and Bonferroni correction. At 18 °C, there was higher expression of VpATP, Tyr, TLR2, and P38 in oysters with PP (WPP) than without PP (WOPP). In contrast, the expression of GnRH-RI, Vasa-like, and SP1b was less WPP than WOPP. It suggests that the PP induces shell repair and immune and inflammatory responses in mantle tissue, while in gonad tissue, it reduces the development of germinal cells. At 24 °C, in RAS-R, there was less expression of SP1b respect RAS-C, and it suggests less cellular division in the gonad. In conclusion, the RAS-R and the PP affect the energetic balance of the Japanese oyster, limiting the energy and reflecting less reproduction effort in oysters from RAS-R at the end of broodstock conditioning.

SRA, biomineralización, Vasa-like, Tyr, ostión RAS, biomineralization, Vasa-like, Tyr, oyster BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA GENÉTICA CITOGENÉTICA ANIMAL CITOGENÉTICA ANIMAL

Relación de adenovirus 36 con la obesidad, expresión de genes (c/ebpB y hif-1A) y la morfologia del tejido adiposo.

JORGE BARRERA ALCOCER (2021, [Tesis de doctorado])

Introducción: Al origen infeccioso de la obesidad se le conoce como ¿infectobesidad¿. Los primeros estudios realizados en modelos animales, como pollos, ratones y primates no humanos, asociaron la presencia de anticuerpos contra HAd36 con el desarrollo de la obesidad y la ganancia de peso, de igual manera los ensayos realizados en preadipocitos (3T3-L1) y células madre adiposas humanas (hASCc) han demostrado que HAd36 se asocia con la expresión de genes implicados en la diferenciación celular y el metabolismo de lípidos. Los estudios realizados para identificar el DNA viral en tejido adiposo son pocos y los resultados inconsistentes. Objetivo: Analizar la presencia del DNA de HAd36 en biopsias de tejido adiposo subcutáneo y su relación con la obesidad, cambios morfológicos de los adipocitos y la expresión de genes adipogénicos y de metabolismo celular. Materiales y Métodos: Se recolectaron un total de 52 biopsias de tejido adiposo subcutáneo de mujeres sometidas a liposucción y/o lipectomia. Se realizó una evaluación antropométrica y clínico-bioquímica. La identificación del DNA de HAd36 se realizó por PCR convencional, la expresión de los genes C/EBPB, HIF-1A y ¿-actina se determinó utilizando sondas TaqMan. La morfología celular se analizó en secciones de tejido adiposo teñidas con H&E, la estimación del número y tamaño de las células se realizó con el software Image J Fiji. Resultados: Se identificó el DNA de HAd36 en 16 muestras de tejido adiposo subcutáneo (31%). La presencia del DNA viral no se asoció con los parámetros antropométricos o metabólicos, tampoco con cambios en la morfología del tejido adiposo. Los niveles de expresión de mRNA para C/EBPB y HIF-1A no mostraron diferencias significativas entre las muestras positivas y negativas al DNA viral (p>0.05). Conclusión: El DNA de HAd36 puede estar presente en el tejido adiposo subcutáneo, pero la presencia del DNA viral no se encontró relacionado con los cambios morfológicos en este tejido, ni con la expresión de genes como C/EBPB y HIF-1A.

BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA GENÉTICA GENÉTICA CLÍNICA

The banana MaWRKY18, MaWRKY45, MaWRKY60 and MaWRKY70 genes encode functional transcription factors and display differential expression in response to defense phytohormones

SERGIO GARCIA LAYNES VIRGINIA AURORA HERRERA VALENCIA Lilia Guadalupe Tamayo Torres VERONICA LIMONES BRIONES FELIPE ALONSO BARREDO POOL FRAY MARTIN BAAS ESPINOLA Angel Alpuche-Solis CARLOS ALBERTO PUCH HAU SANTY PERAZA ECHEVERRIA (2022, [Artículo])

WRKY transcription factors (TFs) play key roles in plant defense responses through phytohormone signaling pathways. However, their functions in tropical fruit crops, especially in banana, remain largely unknown. Several WRKY genes from the model plants rice (OsWRKY45) and Arabidopsis (AtWRKY18, AtWRKY60, AtWRKY70) have shown to be attractive TFs for engineering disease resistance. In this study, we isolated four banana cDNAs (MaWRKY18, MaWRKY45, MaWRKY60, and MaWRKY70) with homology to these rice and Arabidopsis WRKY genes. The MaWRKY cDNAs were isolated from the wild banana Musa acuminata ssp. malaccensis, which is resistant to several diseases of this crop and is a progenitor of most banana cultivars. The deduced amino acid sequences of the four MaWRKY cDNAs revealed the presence of the conserved WRKY domain of ~60 amino acids and a zinc-finger motif at the N-terminus. Based on the number of WRKY repeats and the structure of the zinc-finger motif, MaWRKY18 and MaWRKY60 belong to group II of WRKY TFs, while MaWRKY45 and MaWRKY70 are members of group III. Their corresponding proteins were located in the nuclei of onion epidermal cells and were shown to be functional TFs in yeast cells. Moreover, expression analyses revealed that the majority of these MaWRKY genes were upregulated by salicylic acid (SA) or methyl jasmonate (MeJA) phytohormones, although the expression levels were relatively higher with MeJA treatment. The fact that most of these banana WRKY genes were upregulated by SA or MeJA, which are involved in systemic acquired resistance (SAR) or induced systemic resistance (ISR), respectively, make them interesting candidates for bioengineering broad-spectrum resistance in this crop. © 2022 by the authors.

BANANA TRANSCRIPTION FACTOR WRKY DEFENSE PHYTOHORMONES SALICYLIC ACID METHYL JASMONATE SAR ISR BROAD-SPECTRUM RESISTANCE BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA GENÉTICA GENÉTICA MOLECULAR DE PLANTAS GENÉTICA MOLECULAR DE PLANTAS

Retención larvaria en abulón azul Haliotis fulgens mediante análisis de parentesco en B.C.S., México

ADRIANA MAX AGUILAR (2014, [Tesis de maestría])

El abulón azul (Haliotis fulgens) es un molusco gasterópodo de importancia comercial en México. Es un recurso de alto valor comercial (70 M en 2011) debido a su gran demanda a nivel internacional; su pesquería constituye una de las actividades más importantes que se desarrollan en la costa occidental de la península de Baja California. Esta pesquería ha sufrido de manera histórica una disminución significativa, por lo que es necesario reforzar y continuar los programas de investigación y manejo actual para garantizar la recuperación de los bancos abuloneros. Un aspecto crucial en el manejo y administración de las pesquerías, es el claro entendimiento del concepto de unidad de stock, el cual requiere un mejor conocimiento del reclutamiento en función de la dinámica larvaria y la conectividad entre bancos (dados por los niveles de retención y dispersión), lo que ampliaría la precisión de las estrategias de manejo actuales. En el caso del abulón se han propuesto dos modelos de conectividad: (1) Las larvas son transportadas a grandes distancias de sus padres y llegan a sustentar otros bancos; y (2) el intercambio larvario entre bancos es limitado y cada banco es un pequeño stock que es autosustentable. En cualquier caso, es necesario determinar si en un banco lo que ocurre preponderantemente es el auto-reclutamiento, o si por el contrario, las larvas provienen principalmente de otros sitios. El objetivo del presente trabajo fue estimar el índice de retención larvaria del abulón azul Haliotis fulgens en dos sitios de la costa occidental de Baja California Sur, mediante el uso de marcadores genéticos tipo microsatélite en análisis de parentesco. Se estandarizaron y caracterizaron nuevos loci microsatélites específicos para H. fulgens con los cuales se determinó el índice de retención larvaria a través de la asignación genética de juveniles a progenitores que se encontraran en el mismo sitio de muestreo mediante análisis de paternidad con el programa CERVUS 3.0. Se obtuvieron 24 loci microsatélites potenciales para utilizarlos en análisis genéticos, de los cuales se caracterizaron 12 loci polimórficos. Se determinó que 11 de ellos son factibles de utilizar en análisis de parentesco por su alto polimorfismo (Número de alelos: de 5-17; HE de 0.69-0.92; PIC de 0.64-0.90) y presentar Equilibrio de Hardy-Weinberg [...]

The green abalone (Haliotis fulgens) is a marine gastropod mollusk in Mexico with a high commercial value (70 M in 2011) because of its high demand in the international market. Its fishery is an important commercial activity in the west coast of Baja California Sur, and has historically suffered a significant decline in its captures. Thus it is necessary to continue research and current management programs to ensure the recovery of abalone in reef systems. A crucial consideration in management and administration of this fishery is a clear understanding of the concept of a stock unit, which requires a better understanding of recruitment on defined spatial scales, depending on the larval dynamics and connectivity among reefs (given by the levels of retention and dispersal). This knowledge will extend the accuracy of current management strategies. Two different models on connectivity have been proposed for abalone: (1) the larvae can be transported for long distances from their parent’s reef and supply other distant reefs; and (2) larval exchange between reefs is limited, and each one is a small self-replenishing bank. In any case, it is necessary to determine whether a reef is mainly maintained by self-recruitment, or if larvae come mainly from other sites. The objective of this work was estimate the proportion of green abalone Haliotis fulgens larval retention in two sites on the western coast of Baja California Sur, using microsatellite markers in paternity analysis. New microsatellite markers in H. fulgens were standardized and characterized. The larval retention was estimated by the proportion of juveniles genetically assigned to the possible parents in the same site by paternity analysis using CERVUS 3.0. Twenty-four microsatellite loci potentially useful in genetic analysis were obtained, from which 12 polymorphic loci were characterized. From these, 11 were useful in paternity analysis due to their high polymorphism (number of alleles: 5-17; HE: 0.69-0.92; PIC: 0.64-0.90) and because they were in Hardy-Weinberg equilibrium [...]

Retención larvaria; microsatélites; auto-reclutamiento BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA GENÉTICA GENÉTICA DE POBLACIONES GENÉTICA DE POBLACIONES

Transcriptome mining provides insights into cell wall metabolism and fiber lignification in Agave tequilana Weber

Luis Fernando Maceda Lopez ELSA BEATRIZ GONGORA CASTILLO Enrique Ibarra-Laclette DALIA C. MORAN VELAZQUEZ AMARANTA GIRON RAMIREZ Matthieu Bourdon José Luis Villalpando Aguilar Gabriela Chavez-Calvillo Toomer John Tang Parastoo Azadi Jorge Manuel Santamaría Fernández Itzel López-Rosas Mercedes G Lopez June Simpson FULGENCIO ALATORRE COBOS (2022, [Artículo])

Resilience of growing in arid and semiarid regions and a high capacity of accumulating sugar-rich biomass with low lignin percentages have placed Agave species as an emerging bioen-ergy crop. Although transcriptome sequencing of fiber-producing agave species has been explored, molecular bases that control wall cell biogenesis and metabolism in agave species are still poorly understood. Here, through RNAseq data mining, we reconstructed the cellulose biosynthesis pathway and the phenylpropanoid route producing lignin monomers in A. tequilana, and evaluated their expression patterns in silico and experimentally. Most of the orthologs retrieved showed differential expression levels when they were analyzed in different tissues with contrasting cellulose and lignin accumulation. Phylogenetic and structural motif analyses of putative CESA and CAD proteins allowed to identify those potentially involved with secondary cell wall formation. RT-qPCR assays revealed enhanced expression levels of AtqCAD5 and AtqCESA7 in parenchyma cells associated with extraxylary fibers, suggesting a mechanism of formation of sclerenchyma fibers in Agave similar to that reported for xylem cells in model eudicots. Overall, our results provide a framework for un-derstanding molecular bases underlying cell wall biogenesis in Agave species studying mechanisms involving in leaf fiber development in monocots. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.

AGAVE CELL WALLS LIGNOCELLULOSE CAD PROTEIN CESA PROTEIN SCLERENCHYMA BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA GENÉTICA GENÉTICA MOLECULAR DE PLANTAS GENÉTICA MOLECULAR DE PLANTAS

Solanum tuberosum Microtuber Development under Darkness Unveiled through RNAseq Transcriptomic Analysis

ELIANA VALENCIA LOZANO LISSET HERRERA ISIDRON Osiel Salvador Recoder-Meléndez Aarón Barraza Celis JOSE LUIS CABRERA PONCE (2022, [Artículo])

"Potato microtuber (MT) development through in vitro techniques are ideal propagules for producing high quality potato plants. MT formation is influenced by several factors, i.e., photoperiod, sucrose, hormones, and osmotic stress. We have previously developed a protocol of MT induction in medium with sucrose (8% w/v), gelrite (6g/L), and 2iP as cytokinin under darkness. To understand the molecular mechanisms involved, we performed a transcriptome-wide analysis. Here we show that 1715 up- and 1624 down-regulated genes were involved in this biological process. Through the protein–protein interaction (PPI) network analyses performed in the STRING database (v11.5), we found 299 genes tightly associated in 14 clusters. Two major clusters of up-regulated proteins fundamental for life growth and development were found: 29 ribosomal proteins (RPs) interacting with 6 PEBP family members and 117 cell cycle (CC) proteins. The PPI network of up-regulated transcription factors (TFs) revealed that at least six TFs–MYB43, TSF, bZIP27, bZIP43, HAT4 and WOX9–may be involved during MTs development. The PPI network of down-regulated genes revealed a cluster of 83 proteins involved in light and photosynthesis, 110 in response to hormone, 74 in hormone mediate signaling pathway and 22 related to aging."

transcriptome-wide analysis, microtubers, potato, Solanum tuberosum, darkness, cell cycle, ribosomal proteins, PEBP family genes, cytokinin BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA GENÉTICA GENÉTICA MOLECULAR DE PLANTAS GENÉTICA MOLECULAR DE PLANTAS

HERENCIA MENDELIANA EN MICROSATÉLITES DE ABULÓN AMARILLO Haliotis corrugata

MENDELIAN INHERITANCE IN PINK ABALONE Haliotis corrugata MICROSATELLITES

HERANÇA MENDELIANA DOS MICROSSATÉLITES DE AMARELO ABULON Haliotis corrugata

NOE DIAZ VILORIA RICARDO PEREZ ENRIQUEZ DANIEL AGUILAR OSUNA Pedro Cruz Hernández (2013, [Artículo])

"En México la pesquería de abulón amarillo (Haliotis corrugata) se ha visto fuertemente afectada por la sobrepesca y factores ambientales. En este contexto, la repoblación de los bancos silvestres mediante liberación de larvas o juveniles producidos en laboratorio se ha vislumbrado como una alternativa para incrementar la producción. Cualquier programa de repoblamiento debe considerar una estrategia de manejo genético que evite la pérdida de diversidad genética y que permita dar seguimiento del pedigrí de los individuos producidos en laboratorio y liberados posteriormente en los bancos naturales. Uno de los requisitos de los marcadores moleculares tipo microsatélites empleados para la asignación de parentesco en análisis de pedigrí es su conformación al modelo de herencia mendeliana. En el presente estudio se analizaron larvas veliger de H. corrugata de tres familias no emparentadas (cada una de hermanos completos) con 11 loci microsatélites para evaluar si sus clases genotípicas se ajustaban a las proporciones esperadas bajo herencia mendeliana. Las proporciones genotípicas de ocho loci (Hco15, Hco19, Hco22, Hco47-2, Hco47-3, Hco194, Hka3 y Hka56) se ajustaron a las proporciones mendelianas esperadas, dos loci (Hco47-1 y Hco97) mostraron desviaciones significativas (P<0,05) y en un locus (Hco16) no se pudo comprobar segregación mendeliana. Para análisis de parentesco futuros en esta especie se recomienda el uso de un grupo de al menos seis loci en el cual deben incluirse Hco19, Hco22, Hco47-2 y Hka3 debido a sus moderados a altos polimorfismos."

"In Mexico, the pink abalone (Haliotis corrugata) fishery has been strongly affected by overfishing and environmental factors. In this context, reseeding of natural beds through the release of hatchery produced larvae or juveniles, has been envisioned as an alternative to increase production. Any reseeding program should take in account a genetic management strategy, which avoids the loss of genetic diversity and enables to follow the pedigree of hatchery produced individuals being released in the natural beds. One of the requisites of microsatellites as molecular markers for parentage assignment is their agreement to Mendelian inheritance model. In the present study, Haliotis corrugata veliger larvae from three unrelated families (of fullsibs each), were analyzed with 11 microsatellite loci, to assess if their genotypic classes were in agreement to expected ratios under Mendelian inheritance. The genotypic ratios of eight loci (Hco15, Hco19, Hco22, Hco47-2, Hco47-3, Hco194, Hka3, and Hka56) were in agreement with Mendelian expected ratios, two loci (Hco47-1 and Hco97) showed significant deviations (P<0.05), and in one locus (Hco16) Mendelian segregation could not be demonstrated. From the results we suggest the use a group of at least 6 loci in subsequent parentage analyses in which loci Hco19, Hco22, Hco47-2 and Hka3 should be included because their moderate to high polymorphisms."

Abalón, Haliotis corrugata, Hermanos Completos, Herencia Mendeliana, Microsatélites pink abalone, microsatellites BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA BIOLOGÍA ANIMAL (ZOOLOGÍA) GENÉTICA ANIMAL GENÉTICA ANIMAL