Repositorio Nacional

En el cuerpo humano se han identificado varias fuentes de células madre; sin embargo, los dientes representan una nueva alternativa para la obtención de estas, debido a su fácil obténción, compatibilidad con biomateriales y escasos conflictos éticos; principalmente se han estudiado los tejidos de pulpa dental y ligamento periodontal, de los cuales se han logrado obtener diferentes tipos celulares que tienen el potencial de ser utilizadas para desarrollar estrategias para el tratamiento de enfermedades o la regeneración de órganos y tejidos.

CELULAS DENTALES LIGAMENTO PERIODONTAL LINAJE CELULAR PULPA DENTAL REGENERACION BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA BIOLOGÍA MOLECULAR BIOLOGÍA MOLECULAR DE PLANTAS BIOLOGÍA MOLECULAR DE PLANTAS

Estudio de la proteína Fibrilarina Like (FBLL1)

JANY VALDES MARTINEZ (2023, [Tesis de maestría])

BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA BIOLOGÍA MOLECULAR BIOLOGÍA MOLECULAR DE PLANTAS BIOLOGÍA MOLECULAR DE PLANTAS

Expresión heterológica y caracterización funcional de un aldehído deshidrogenasa de Bixa orellana (BoALDH3H1-1) potencialmente implicada en la biosíntesis de bixina

GABRIELA DEL CARMEN VAZQUEZ GOMEZ (2023, [Tesis de maestría])

BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA BIOLOGÍA MOLECULAR BIOLOGÍA MOLECULAR DE PLANTAS BIOLOGÍA MOLECULAR DE PLANTAS

Efecto de componentes virales sobre la actividad ribonucleasa de la fibrilarina

CARLO STEFANO DECLER CARRASCO (2023, [Tesis de doctorado])

BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA BIOLOGÍA MOLECULAR BIOLOGÍA MOLECULAR DE PLANTAS BIOLOGÍA MOLECULAR DE PLANTAS

Localización celular de la síntesis de alcaloides en Argemone mexicana L.

JOSE IGNACIO LAINES HIDALGO (2024, [Tesis de doctorado])

BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA BIOLOGÍA MOLECULAR BIOLOGÍA MOLECULAR DE PLANTAS BIOLOGÍA MOLECULAR DE PLANTAS

The banana MaWRKY18, MaWRKY45, MaWRKY60 and MaWRKY70 genes encode functional transcription factors and display differential expression in response to defense phytohormones

SERGIO GARCIA LAYNES VIRGINIA AURORA HERRERA VALENCIA Lilia Guadalupe Tamayo Torres VERONICA LIMONES BRIONES FELIPE ALONSO BARREDO POOL FRAY MARTIN BAAS ESPINOLA Angel Alpuche-Solis CARLOS ALBERTO PUCH HAU SANTY PERAZA ECHEVERRIA (2022, [Artículo])

WRKY transcription factors (TFs) play key roles in plant defense responses through phytohormone signaling pathways. However, their functions in tropical fruit crops, especially in banana, remain largely unknown. Several WRKY genes from the model plants rice (OsWRKY45) and Arabidopsis (AtWRKY18, AtWRKY60, AtWRKY70) have shown to be attractive TFs for engineering disease resistance. In this study, we isolated four banana cDNAs (MaWRKY18, MaWRKY45, MaWRKY60, and MaWRKY70) with homology to these rice and Arabidopsis WRKY genes. The MaWRKY cDNAs were isolated from the wild banana Musa acuminata ssp. malaccensis, which is resistant to several diseases of this crop and is a progenitor of most banana cultivars. The deduced amino acid sequences of the four MaWRKY cDNAs revealed the presence of the conserved WRKY domain of ~60 amino acids and a zinc-finger motif at the N-terminus. Based on the number of WRKY repeats and the structure of the zinc-finger motif, MaWRKY18 and MaWRKY60 belong to group II of WRKY TFs, while MaWRKY45 and MaWRKY70 are members of group III. Their corresponding proteins were located in the nuclei of onion epidermal cells and were shown to be functional TFs in yeast cells. Moreover, expression analyses revealed that the majority of these MaWRKY genes were upregulated by salicylic acid (SA) or methyl jasmonate (MeJA) phytohormones, although the expression levels were relatively higher with MeJA treatment. The fact that most of these banana WRKY genes were upregulated by SA or MeJA, which are involved in systemic acquired resistance (SAR) or induced systemic resistance (ISR), respectively, make them interesting candidates for bioengineering broad-spectrum resistance in this crop. © 2022 by the authors.

BANANA TRANSCRIPTION FACTOR WRKY DEFENSE PHYTOHORMONES SALICYLIC ACID METHYL JASMONATE SAR ISR BROAD-SPECTRUM RESISTANCE BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA GENÉTICA GENÉTICA MOLECULAR DE PLANTAS GENÉTICA MOLECULAR DE PLANTAS

Solanum tuberosum Microtuber Development under Darkness Unveiled through RNAseq Transcriptomic Analysis

ELIANA VALENCIA LOZANO LISSET HERRERA ISIDRON Osiel Salvador Recoder-Meléndez Aarón Barraza Celis JOSE LUIS CABRERA PONCE (2022, [Artículo])

"Potato microtuber (MT) development through in vitro techniques are ideal propagules for producing high quality potato plants. MT formation is influenced by several factors, i.e., photoperiod, sucrose, hormones, and osmotic stress. We have previously developed a protocol of MT induction in medium with sucrose (8% w/v), gelrite (6g/L), and 2iP as cytokinin under darkness. To understand the molecular mechanisms involved, we performed a transcriptome-wide analysis. Here we show that 1715 up- and 1624 down-regulated genes were involved in this biological process. Through the protein–protein interaction (PPI) network analyses performed in the STRING database (v11.5), we found 299 genes tightly associated in 14 clusters. Two major clusters of up-regulated proteins fundamental for life growth and development were found: 29 ribosomal proteins (RPs) interacting with 6 PEBP family members and 117 cell cycle (CC) proteins. The PPI network of up-regulated transcription factors (TFs) revealed that at least six TFs–MYB43, TSF, bZIP27, bZIP43, HAT4 and WOX9–may be involved during MTs development. The PPI network of down-regulated genes revealed a cluster of 83 proteins involved in light and photosynthesis, 110 in response to hormone, 74 in hormone mediate signaling pathway and 22 related to aging."

transcriptome-wide analysis, microtubers, potato, Solanum tuberosum, darkness, cell cycle, ribosomal proteins, PEBP family genes, cytokinin BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA GENÉTICA GENÉTICA MOLECULAR DE PLANTAS GENÉTICA MOLECULAR DE PLANTAS