New laccase-producing fungi isolates with biotechnological potential in dye decolorization

RAUL TAPIA TUSSELL DAISY DE LA CARIDAD PEREZ BRITO RAFAEL ANTONIO ROJAS HERRERA ALBERTO CORTES VELAZQUEZ GERARDO ENRIQUE RIVERA MUÑOZ SARA ELENA SOLIS PEREIRA (2011)

Article

DYE DECOLORIZATION LACCASE PCR FINGERPRINTING TRAMETES HIRSUTA BIOLOGÍA Y QUÍMICA

Laccase gene expression and vinasse biodegradation by Trametes hirsuta strain Bm-2

RAUL TAPIA TUSSELL DAISY DE LA CARIDAD PEREZ BRITO CLAUDIA GUADALUPE TORRES CALZADA ALBERTO CORTES VELAZQUEZ LILIANA MARIA ALZATE GAVIRIA RUBI DEL ROSARIO CHABLE VILLACIS SARA ELENA SOLIS PEREIRA (2015)

Vinasse is the dark-colored wastewater that is generated by bioethanol distilleries from feedstock molasses. The vinasse that is generated from molasses contains high amounts of pollutants, including phenolic compounds and melanoindin. The goal of this work was to study the expression of laccase genes in the Trametes hirsuta strain Bm-2, isolated in Yucatan, Mexico, in the presence of phenolic compounds, as well as its effectiveness in removing colorants from vinasse. In the presence of all phenolic compounds tested (guaiacol, ferulic acid, and vanillic acid), increased levels of laccase-encoding mRNA were observed. Transcript levels in the presence of guaiacol were 40 times higher than those in the control. The lcc1 and lcc2 genes of T. hirsuta were differentially expressed; guaiacol and vanillin induced the expression of both genes, whereas ferulic acid only induced the expression of lcc2. The discoloration of vinasse was concomitant with the increase in laccase activity. The highest value of enzyme activity (2543.7 U/mL) was obtained in 10% (v/v) vinasse, which corresponded to a 69.2% increase in discoloration. This study demonstrates the potential of the Bm-2 strain of T. hirsuta for the biodegradation of vinasse.

Article

TRAMETES HIRSUTA LACASSES GENE EXPRESSION BIODEGRADATION VINASSE BIOLOGÍA Y QUÍMICA BIOLOGÍA Y QUÍMICA

Laccase gene expression and vinasse biodegradation by Trametes hirsuta strain Bm-2

RAUL TAPIA TUSSELL DAISY DE LA CARIDAD PEREZ BRITO CLAUDIA GUADALUPE TORRES CALZADA ALBERTO CORTES VELAZQUEZ LILIANA MARIA ALZATE GAVIRIA RUBI DEL ROSARIO CHABLE VILLACIS SARA ELENA SOLIS PEREIRA (2015)

Vinasse is the dark-colored wastewater that is generated by bioethanol distilleries from feedstock molasses. The vinasse that is generated from molasses contains high amounts of pollutants, including phenolic compounds and melanoindin. The goal of this work was to study the expression of laccase genes in the Trametes hirsuta strain Bm-2, isolated in Yucatan, Mexico, in the presence of phenolic compounds, as well as its effectiveness in removing colorants from vinasse. In the presence of all phenolic compounds tested (guaiacol, ferulic acid, and vanillic acid), increased levels of laccase-encoding mRNA were observed. Transcript levels in the presence of guaiacol were 40 times higher than those in the control. The lcc1 and lcc2 genes of T. hirsuta were differentially expressed; guaiacol and vanillin induced the expression of both genes, whereas ferulic acid only induced the expression of lcc2. The discoloration of vinasse was concomitant with the increase in laccase activity. The highest value of enzyme activity (2543.7 U/mL) was obtained in 10% (v/v) vinasse, which corresponded to a 69.2% increase in discoloration. This study demonstrates the potential of the Bm-2 strain of T. hirsuta for the biodegradation of vinasse.

Article

TRAMETES HIRSUTA LACASSES GENE EXPRESSION BIODEGRADATION VINASSE BIOLOGÍA Y QUÍMICA

Biological pretreatment of mexican caribbean macroalgae consortiums using Bm-2 strain (Trametes hirsuta) and its enzymatic broth to improve biomethane potential

RAUL TAPIA TUSSELL JULIO ALBERTO AVILA ARIAS Jorge Arturo Domínguez Maldonado DAVID VALERO MORALES Edgar Olguin Maciel Daisy de la Caridad Pérez Brito Liliana María Alzate Gaviria (2018)

The macroalgae consortium biomass in the Mexican Caribbean represents an emerging and promising biofuel feedstock. Its biological pretreatment and potential for energetic conversion to biomethane were investigated, since some macroalgae have hard cell walls that present an obstacle to efficient methane production when those substrates are used. It has been revealed by anaerobic digestion assays that pretreatment with a Bm-2 strain (Trametes hirsuta) isolated from decaying wood in Yucatan, Mexico was 104 L CH4 kg·VS−1; In fact, the fungal pretreatment produced a 20% increase in methane yield, with important amounts of alkali metals Ca, K, Mg, Na of 78 g/L, ash 35.5% and lignin 15.6%. It is unlikely that high concentrations of ash and alkali metals will produce an ideal feedstock for combustion or pyrolysis, but they can be recommended for a biological process.

Article

BIOLOGICAL PRETREATMENT MACROALGAE CONSORTIUM BIOMETHANE POTENTIAL TRAMETES HIRSUTA BIOLOGÍA Y QUÍMICA BIOLOGÍA Y QUÍMICA