Title
Phylogenetic relationships of Pseudo-nitzschia subpacifica (Bacillariophyceae) from the Mexican Pacific, and its production of domoic acid in culture
Author
Sonia Quijano
Contributor
ARAMIS OLIVOS ORTIZ (Collaborator)
ERNESTO GARCIA MENDOZA (Collaborator)
YAIREB ALEJANDRA SANCHEZ BRAVO (Collaborator)
RAMON SOSA AVALOS (Collaborator)
Nathalli Salas Marias (Collaborator)
Hong Chang Lim (Collaborator)
Access level
Open Access
Alternative identifier
doi: https://doi.org/10.1371/journal.pone.0231902
Subjects
domoic acid, domoic acid, kainic acid, Article, cell growth, controlled study, diatom, Mexico, morphology, nonhuman, Pacific Ocean, phylogeny, plant cell, plant growth, Pseudo nitzschia, toxin analysis, cell culture technique, classification, diatom, - (SCOPUS) CIENCIAS FÍSICO MATEMÁTICAS Y CIENCIAS DE LA TIERRA - (CTI) CIENCIAS DE LA TIERRA Y DEL ESPACIO - (CTI) OCEANOGRAFÍA - (CTI) OCEANOGRAFÍA - (CTI)
Summary or description
Pseudo-nitzschia is a cosmopolitan genus, some species of which can produce domoic acid (DA), a neurotoxin responsible for the Amnesic Shellfish Poisoning (ASP). In this study, we identified P. subpacifica for the first time in Todos Santos Bay and Manzanillo Bay, in the Mexican Pacific using SEM and molecular methods. Isolates from Todos Santos Bay were cultivated under conditions of phosphate sufficiency and deficiency at 16°C and 22°C to evaluate the production of DA. This toxin was detected in the particulate (DAp) and dissolved (DAd) fractions of the cultures during the exponential and stationary phases of growth of the cultures. The highest DA concentration was detected during the exponential phase grown in cells maintained in P-deficient medium at 16°C (1.14 ± 0.08 ng mL-1 DAd and 4.71 ± 1.11 × 10−5 ng cell-1 of DAp). In P-sufficient cultures DA was higher in cells maintained at 16°C (0.25 ± 0.05 ng mL-1 DAd and 9.41 ± 1.23 × 10−7 ng cell-1 of DAp) than in cells cultured at 22°C. Therefore, we confirm that P. subpacifica can produce DA, especially under P-limited conditions that could be associated with extraordinary oceanographic events such as the 2013–2016 "Blob" in the northeastern Pacific Ocean. This event altered local oceanographic conditions and possibly generated the presence of potential harmful species in areas with economic importance on the Mexican Pacific coast. © 2020 Quijano-Scheggia et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Publisher
Alberto Amato, IRIG-CEA Grenoble, France
Publish date
2020
Publication type
Article
Publication version
Published Version
Information Resource
Format
application/pdf
Source
PLoS ONE, Vol.15, No.4, Pags. 1-16
Language
English
Citation suggestion
Quijano-Scheggia SI, Olivos-Ortiz A, Garcia-Mendoza E, Sánchez-Bravo Y, Sosa-Avalos R, Salas Marias N, et al. (2020) Phylogenetic relationships of Pseudo-nitzschia subpacifica (Bacillariophyceae) from the Mexican Pacific, and its production of domoic acid in culture. PLoS ONE 15(4): e0231902. https://doi.org/10.1371/journal.pone.0231902
Source repository
Repositorio Institucional CICESE
Downloads
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