Título
Phylogenetic relationships of Pseudo-nitzschia subpacifica (Bacillariophyceae) from the Mexican Pacific, and its production of domoic acid in culture
Autor
Sonia Quijano
Colaborador
ARAMIS OLIVOS ORTIZ (Colaborador)
ERNESTO GARCIA MENDOZA (Colaborador)
YAIREB ALEJANDRA SANCHEZ BRAVO (Colaborador)
RAMON SOSA AVALOS (Colaborador)
Nathalli Salas Marias (Colaborador)
Hong Chang Lim (Colaborador)
Nivel de Acceso
Acceso Abierto
Identificador alterno
doi: https://doi.org/10.1371/journal.pone.0231902
Materias
domoic acid, domoic acid, kainic acid, Article, cell growth, controlled study, diatom, Mexico, morphology, nonhuman, Pacific Ocean, phylogeny, plant cell, plant growth, Pseudo nitzschia, toxin analysis, cell culture technique, classification, diatom, - (SCOPUS) CIENCIAS FÍSICO MATEMÁTICAS Y CIENCIAS DE LA TIERRA - (CTI) CIENCIAS DE LA TIERRA Y DEL ESPACIO - (CTI) OCEANOGRAFÍA - (CTI) OCEANOGRAFÍA - (CTI)
Resumen o descripción
Pseudo-nitzschia is a cosmopolitan genus, some species of which can produce domoic acid (DA), a neurotoxin responsible for the Amnesic Shellfish Poisoning (ASP). In this study, we identified P. subpacifica for the first time in Todos Santos Bay and Manzanillo Bay, in the Mexican Pacific using SEM and molecular methods. Isolates from Todos Santos Bay were cultivated under conditions of phosphate sufficiency and deficiency at 16°C and 22°C to evaluate the production of DA. This toxin was detected in the particulate (DAp) and dissolved (DAd) fractions of the cultures during the exponential and stationary phases of growth of the cultures. The highest DA concentration was detected during the exponential phase grown in cells maintained in P-deficient medium at 16°C (1.14 ± 0.08 ng mL-1 DAd and 4.71 ± 1.11 × 10−5 ng cell-1 of DAp). In P-sufficient cultures DA was higher in cells maintained at 16°C (0.25 ± 0.05 ng mL-1 DAd and 9.41 ± 1.23 × 10−7 ng cell-1 of DAp) than in cells cultured at 22°C. Therefore, we confirm that P. subpacifica can produce DA, especially under P-limited conditions that could be associated with extraordinary oceanographic events such as the 2013–2016 "Blob" in the northeastern Pacific Ocean. This event altered local oceanographic conditions and possibly generated the presence of potential harmful species in areas with economic importance on the Mexican Pacific coast. © 2020 Quijano-Scheggia et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Editor
Alberto Amato, IRIG-CEA Grenoble, France
Fecha de publicación
2020
Tipo de publicación
Artículo
Versión de la publicación
Versión publicada
Recurso de información
Formato
application/pdf
Fuente
PLoS ONE, Vol.15, No.4, Pags. 1-16
Idioma
Inglés
Sugerencia de citación
Quijano-Scheggia SI, Olivos-Ortiz A, Garcia-Mendoza E, Sánchez-Bravo Y, Sosa-Avalos R, Salas Marias N, et al. (2020) Phylogenetic relationships of Pseudo-nitzschia subpacifica (Bacillariophyceae) from the Mexican Pacific, and its production of domoic acid in culture. PLoS ONE 15(4): e0231902. https://doi.org/10.1371/journal.pone.0231902
Repositorio Orígen
Repositorio Institucional CICESE
Descargas
17