Título

PHOTOCHEMICAL SYNTHESIS OF HEPARIN-BASED GOLD NANOPARTICLES AND THEIR USE IN SURFACE ENHANCED RAMAN SPECTROSCOPY

Autor

María del Pilar Rodríguez-Torres

Colaborador

Luis Armando Diaz-Torres (Asesor de tesis)

Nivel de Acceso

Acceso Abierto

Licencia

http://creativecommons.org/licenses/by-nc-nd/4.0

Materias

UV-VIS SPECTROSCOPY, SCANNING ELECTRON MICROSCOPY, NANOPARTICLES, RAMAN SPECTROSCOPY - (AUTOR) CIENCIAS FÍSICO MATEMÁTICAS Y CIENCIAS DE LA TIERRA - (CTI) FÍSICA - (CTI) ÓPTICA - (CTI)

Resumen o descripción

"This dissertation deals with gold nanoparticle green synthesis in aqueous solution using heparin, as a reducing and stabilizing agent, without other reagents through photochemistry, and their usage as surface enhanced Raman colloidal substrates. This work is divided in four parts: The first one is about proposing the photochemical synthesis method used to prepare the heparinbased gold nanoparticles in aqueous solution using UVA light (λ=366 nm). Two types of experiments were carried out: The nanoparticles were synthesized using reactive and pharmaceutical-grade heparins. Nanoparticle characterization was carried out with UV-Vis spectroscopy and Scanning Electron Microscopy. In the second one, a nanoparticle formation mechanism is proposed with the aid of UV-Vis and FTIR spectroscopies and pH measurements.

In the third part, surface enhanced Raman activity is investigated, finding that reactive-grade heparin gold nanoparticles yield good Raman signals and the pharmaceutical-grade ones do not. The probe molecules used were differently charged dyes: Methylene Blue (-), Rosebengal (+) and Neutral Red (+-); as well as proteins (GP120 and Bovine Serum Albumin). There are no reports on heparin-gold nanoparticles being used for this purpose to our knowledge. In the final section, this dissertation´s contributions are presented as a closure to the research that was performed."

Fecha de publicación

septiembre de 2015

Tipo de publicación

Tesis de doctorado

Recurso de información

http://cio.repositorioinstitucional.mx/jspui/handle/1002/426

Formato

application/pdf

Idioma

Inglés

Cobertura

León, Guanajuato

Audiencia

Público en general

Sugerencia de citación

Rodríguez-Torres (2015). "Photochemical synthesis of heparin-based gold nanoparticles and their use in Surface Enhanced Raman Spectroscopy". Tesis de Doctorado en Ciencias (Óptica). Centro de Investigaciones en Óptica, A.C. León, Guanajuato. 107 pp.

Repositorio Orígen

REPOSITORIO INSTITUCIONAL DEL CIO

Descargas

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