Author: Luis Daniel Espinosa Chaurand

Cronobacter sakasakii un peligro potencial para la salud proveniente de los alimentos

Cronobacter sakasakii a potential health hazard in food

ALEJANDRO DE JESUS CORTES SANCHEZ Luis Daniel Espinosa Chaurand (2018)

"Las enfermedades transmitidas por alimentos (ETA) son consideradas tema de relevancia en salud pública global debido a la tasa de incidencia, mortalidad y secuelas negativas económico sociales. Algunas ETA presentan como agentes causales a hongos, virus, parásitos y bacterias; siendo estas últimas comúnmente asociadas a estos padecimientos. Cronobacter sakasakii es considerado un patógeno alimentario de graves daños a la salud incluso la muerte, principalmente en lactantes derivado del consumo de alimentos en polvo. Se han desarrollado diferentes estrategias de vigilancia, control y prevención involucrándose organizaciones internacionales, autoridades gubernamentales, industria de alimentos y consumidor alrededor del mundo a fin de reducir su incidencia y proteger la salud pública. La finalidad del presente documento es mostrar una perspectiva general de las enfermedades transmitidas por alimentos y específicamente, las derivadas por bacterias del género Cronobacter spp., como C. sakasakii abarcando desde sus características metabólicas, métodos para su detección en los alimentos, hasta la normatividad en control y prevención para la protección de la salud. Así mismo se muestra una potencial dualidad al reportarse en diversas investigaciones alrededor del mundo que más allá del carácter patógeno de estos microorganismos, algunos pueden mostrar potenciales aplicaciones biotecnológicas principalmente en el área ambiental e industria del petróleo."

"Foodborne Diseases (FD) are considered a relevant issue in global public health due to the incidence rate, mortality and negative social economic consequences. Some FD present as causal agents to fungi, viruses, parasites and bacteria; being the latter commonly associated with these conditions. Cronobacter sakasakii is considered a foodborne pathogen of serious damage to health including death, mainly in infants derived from the consumption of powdered food. Different surveillance, control and prevention strategies have been developed involving international organizations, government authorities, food industry and consumers around the world in order to reduce their incidence and protect public health. The purpose of this document is to show a general perspective of foodborne diseases and specifically, those derived by bacteria of the genus Cronobacter spp., As C. sakasakii covering aspects from their metabolic characteristics, methods for their detection in foods, regulations in control and prevention for the protection of health. It also shows a potential duality to be reported in various research around the world that beyond the pathogenic nature of these microorganisms, some may show potential biotechnological applications mainly in the environmental area and oil industry."

Article

Cronobacter sakasakii, alimentos, biosurfactantes food safety, biosurfactants MEDICINA Y CIENCIAS DE LA SALUD CIENCIAS MÉDICAS CIENCIAS DE LA NUTRICIÓN ENFERMEDADES DE LA NUTRICIÓN ENFERMEDADES DE LA NUTRICIÓN

Foods, fish and salmonellosis

ALEJANDRO DE JESUS CORTES SANCHEZ Luis Daniel Espinosa Chaurand (2018)

"Foodborne diseases are those caused by the consumption of water and food contaminated by different causal agents such as viruses, bacteria, parasites, toxins, among others, being considered an important public health problem global due to its incidence and mortality and for several years for the isolation of microorganisms that cause these diseases resistant to antimicrobials. Salmonella species is considered a food pathogen frequently responsible for infectious outbreaks through the consumption of contaminated food, also presenting resistance to different antimicrobials. Fishery products are recognized as an important source of food, nutrition, income and a source of livelihood for a large part of the world's population. However, fish is also considered to be a vehicle that transmits different pathogens (Salmonella spp., Shigella species, Escherichia coli, Listeria monocytogenes among others) mainly due to inadequate hygiene practices along the food chain. The purpose of this article is to show in a general way a perspective of foodborne diseases, specifically those caused by bacteria of the genus Salmonella spp., through fish such as tilapia, the control and prevention measures of these pathogens in food, the phenomenon of resistance to antimicrobials by these bacteria isolated in food and fish around the world that exacerbates the problem in food safety and public health."

Article

Salmonella, food, fish, processing, antimicrobial CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA CIENCIAS AGRARIAS PECES Y FAUNA SILVESTRE PISCICULTURA PISCICULTURA

Effect of protein level in diet and feeding schedule on the digestive enzymatic activity of Macrobrachium tenellum juveniles

Luis Daniel Espinosa Chaurand olimpia victoria carrillo farnés FERNANDO VEGA VILLASANTE Héctor Gerardo Nolasco Soria (2019)

"This study assessed the effect of protein level in diet and feeding schedule on the digestive enzymatic activity of Macrobrachium tenellum juveniles. Freshwater prawn juveniles (0.31 ± 0.004 g and 32.48 ± 0.27 mm) were fed diets with 20, 25, 30, 35 and 40% crude protein (CP) (p/p). In a second experiment, feeding schedules were assessed at 08:00, 14:00 and 20:00 h with feed at 30% CP with juveniles of 0.22 ± 0.002 g and 29.36 ± 0.28 mm. In both experiments, five organisms were sampled after 60 days of treatment in a time-lapse of one hour (08:00 to 09:00 h) per experimental tank and treatment. The enzymatic extract was obtained from the complete organisms to determine specific general protease, trypsin, chymotrypsin, amylase and lipase activities. Greater general protease, trypsin and lipase activities were observed in feed at 40% CP. The feeding schedules with greater general protease, trypsin, lipase and amylase activities were found at 14:00 and 20:00 h. Chymotrypsin did not show differences among treatments at CP levels or feeding schedules. Protein level in diets for M. tenellum modified basal digestive enzymatic activity and increased proteolytic and lipolytic activities, while it decreased amylolytic activity as the inclusion of CP level increased in the diet; likewise, the activity was modified by feeding schedule, showing a direct correlation between the increase in digestive enzymatic activity and food offering at the end of the day."

Article

Macrobrachium tenellum, prawn, feeding schedule, digestive enzymes, digestive physiology CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA CIENCIAS AGRARIAS PRODUCCIÓN ANIMAL NUTRICIÓN NUTRICIÓN

NIVELES DE PROTEÍNA EN DIETAS BALANCEADAS PARA PRE-ADULTOS DE MACROBRACHIUM TENELLUM

LUIS DANIEL ESPINOSA CHAURAND FERNANDO VEGA VILLASANTE HECTOR GERARDO NOLASCO SORIA olimpia victoria carrillo farnés (2013)

"The objective was to evaluate for 60 days the effect of five concentrations of protein in balanced diets (20, 25, 30, 35 and 40% CP) on the growth and survival of pre-adult M. tenellum. No significant difference in survival between treatments, the average was 80.00 ± 6.67%. At the same time no evidence was found to suggest that the increase in the percentage of crude protein inclusion promotes growth of organisms in experimentation. Under the experimental conditions raised, pre-adult organisms of this species there is no difference in growth by the inclusion level of dietary protein, suggesting that the protein requirement indicated for this stage is 25% of PC."

"El objetivo fue evaluar por 60 días el efecto de cinco concentraciones de proteína en dietas balanceadas (20, 25, 30, 35 y 40 % de PC) sobre el crecimiento y sobrevivencia de pre-adultos de M. tenellum. No existieron diferencias significativas en la sobrevivencia entre tratamientos, su promedio fue de 80.00±6.67%. A su vez no se encontraron evidencias que sugieran que el incremento en el porcentaje de inclusión de proteína cruda favorezca el crecimiento de los organismos en experimentación. Bajo las condiciones experimentales planteadas, en organismos pre-adultos de esta especie no existe diferencia en el crecimiento por el nivel de inclusión de proteína en la dieta, sugiriéndose que el requerimiento proteico indicado para esta etapa es de 25% de PC."

Article

Macrobrachium, nivel de proteína, alimentos, pre-adultos protein, diets CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA CIENCIAS AGRARIAS PRODUCCIÓN ANIMAL NUTRICIÓN NUTRICIÓN

NIVELES DE PROTEÍNA EN DIETAS BALANCEADAS PARA PRE-ADULTOS DE MACROBRACHIUM TENELLUM

LUIS DANIEL ESPINOSA CHAURAND FERNANDO VEGA VILLASANTE HECTOR GERARDO NOLASCO SORIA olimpia victoria carrillo farnés (2013)

"The objective was to evaluate for 60 days the effect of five concentrations of protein in balanced diets (20, 25, 30, 35 and 40% CP) on the growth and survival of pre-adult M. tenellum. No significant difference in survival between treatments, the average was 80.00 ± 6.67%. At the same time no evidence was found to suggest that the increase in the percentage of crude protein inclusion promotes growth of organisms in experimentation. Under the experimental conditions raised, pre-adult organisms of this species there is no difference in growth by the inclusion level of dietary protein, suggesting that the protein requirement indicated for this stage is 25% of PC."

"El objetivo fue evaluar por 60 días el efecto de cinco concentraciones de proteína en dietas balanceadas (20, 25, 30, 35 y 40 % de PC) sobre el crecimiento y sobrevivencia de pre-adultos de M. tenellum. No existieron diferencias significativas en la sobrevivencia entre tratamientos, su promedio fue de 80.00±6.67%. A su vez no se encontraron evidencias que sugieran que el incremento en el porcentaje de inclusión de proteína cruda favorezca el crecimiento de los organismos en experimentación. Bajo las condiciones experimentales planteadas, en organismos pre-adultos de esta especie no existe diferencia en el crecimiento por el nivel de inclusión de proteína en la dieta, sugiriéndose que el requerimiento proteico indicado para esta etapa es de 25% de PC."

Article

Macrobrachium, nivel de proteína, alimentos, pre-adultos protein, diets CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA CIENCIAS AGRARIAS PRODUCCIÓN ANIMAL NUTRICIÓN NUTRICIÓN

Efecto del nivel proteico de la dieta sobre el desarrollo de juveniles de Macrobrachium tenellum (Smith, 1871)

Effect of dietary protein level on the development of juveniles of Macrobrachiun tenellum (Smith, 1871)

LUIS DANIEL ESPINOSA CHAURAND HECTOR GERARDO NOLASCO SORIA olimpia victoria carrillo farnés FERNANDO VEGA VILLASANTE (2012)

"Objetivo. Evaluar el efecto de cinco niveles de proteína cruda (PC) en alimentos balanceados sobre el crecimiento, sobrevivencia y tasa de conversión alimenticia (FCA) en juveniles de Macrobrachium tenellum. Materiales y métodos. Se alimentó por 60 días a juveniles de M. tenellum (0.31±0.01 g y 32.62±1.10 mm) con niveles de 20, 25, 30, 35 y 40% de PC en el alimento. Los organismos fueron distribuidos al azar en 15 tinas experimentales de 64 L (15 org./tina) bajo condiciones controladas (5.95±0.41 ppm de oxígeno, 29.89±0.72ºC, y pH 8.44±0.15) y alimentados con el 10% de su peso vivo. Resultados. El porcentaje de sobrevivencia fue del 98.22±3.96% sin diferencias significativas entre los tratamientos (p>0.05). Los organismos alimentados con un 40% de PC tuvieron un peso significativamente mayor (p<0.05) respecto a los demás tratamientos (cambio de peso de 0.54±0.02g; incremento de peso de 173.60±12.99%; y tasa de crecimiento específico de 1.68±0.08). El FCA fue significativamente mejor (p<0.05) en los organismos alimentados con 35 y 40% de PC (2.85±0.18 y 2.40±0.05, respectivamente) que los demás tratamientos. Conclusiones. Los organismos juveniles de M. tenellum alimentados con niveles altos de proteína (40%), se desarrollaron más rápido que organismos que recibieron una menor concentración de proteína bajo las condiciones experimentales establecidas en este estudio."

"Objective. To evaluate the effect of five levels of crude protein (CP) in balanced feed on the survival, growth and feed conversion ratio (FCA) in juveniles of Macrobrachium tenellum. Materials and methods. Juveniles of M. tenellum (0.31±0.01g and 32.62±1.10mm) were fed for 60 days with 20, 25, 30, 35 and 40% of CP in feed. The organisms were randomly distributed in 15 experimental tanks (15 org /tank) under controlled conditions (5.95±0.41 ppm of oxygen, 29.89 ± 0.72 °C, and pH 8.44±0.15) and fed with 10% of its live weight. Results. The survival percentage was 98.22±3.96% with no statistical difference between treatments (p>0.05). The organisms fed with 40% CP in their diet had a significantly higher weight (p<0.05) compared to the other treatments (weight change of 0.54±0.02g; weight increase 173.60±12.99%, and specific growth rate of 1.68±0.08). The FCA was significantly better (p<0.05) in organisms fed with 35 and 40% CP (2.85±0.18 and 2.40±0.05, respectively) than other treatments. Conclusions. Juveniles of M. tenellum fed with high protein levels (40%) developed faster than organisms which received a lower concentration of the protein under the experimental conditions established for this study."

Article

Alimentos, crecimiento, Macrobrachium, nutrición, proteína de la dieta Dietary protein, foods, growth, nutrition BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA BIOLOGÍA ANIMAL (ZOOLOGÍA) NUTRICIÓN NUTRICIÓN

Legionella spp., A HAZARD TO HUMAN HEALTH

Legionella spp., UN PELIGRO PARA LA SALUD HUMANA

ALEJANDRO DE JESUS CORTES SANCHEZ Luis Daniel Espinosa Chaurand MAYRA DIAZ RAMIREZ (2018)

"Objective: The aim of this study was to provide a review that gives a general perspective of legionellosis and the different factors associated with this disease, such as the incidence, causal agents, treatment, control and prevention methods used globally in order to safeguard public health. Methodology: To develop this document, the search and analysis of pertinent information was carried out in different scientific and academic databases such as Scielo, Scopus, Latindex, Redalyc, Google Scholar, among others. Results: Legionellosis is caused by species of the genus Legionella spp., with L. pneumophila being commonly associated to clinical cases. Analytical methods have been developed for their detection, as well as sanitary regulations for water and hydraulic facilities for human use, in order to mitigate their impact on the population. Implications in the study: The causal agents of legionellosis inhabit aquatic ecosystems, and are capable of resisting diverse physicochemical conditions; therefore, they have been detected in hydraulic systems and equipment for human use, thus generating their dispersion and potential infection when inhaled through small drops of water (sprays). Conclusions: Legionella spp. is an emerging pathogen of importance in public health mainly in developed countries, due to its incidence, complications and mortality. It has been established that the prevention and control of legionellosis require joint actions in the microbiological control of water, development, dissemination, implementation and monitoring of the application of regular cleaning and disinfection programs by governmental entities, international health organizations, academia, industry and the general population."

"Objetivo: Brindar una revisión que aporte una perspectiva general de la legionelosis y de los diferentes factores asociados a esta enfermedad tales como la incidencia, agentes causales, terapéutica, métodos de control y prevención utilizados a nivel global a fin de salvaguardar la salud pública. Metodología: Para el desarrollo del presente documento se realizó la búsqueda y análisis de información pertinente en diferentes bases de datos científicas y académicas como Scielo, Scopus, Latindex, Redalyc, Google Académico entre otras. Resultados: La legionelosis es causada por especies del género Legionella spp., siendo L. pneumophila comúnmente asociada a casos clínicos. Se han desarrollado métodos analíticos para su detección, así como regulaciones sanitarias al agua e instalaciones hidráulicas para uso humano, a fin de mitigar su incidencia en la población. Implicaciones en el estudio: Los agentes causales de legionelosis habitan los ecosistemas acuáticos, y son capaces de resistir diversas condiciones fisicoquímicas por ende se han detectado en sistemas y equipos hidráulicos de uso humano, generando así su dispersión y potencial infección al ser inhaladas a través de pequeñas gotas de agua (aerosoles)."

Article

Legionellosis, water, hydraulic facilities, public health Legionelosis, agua, instalaciones hidráulicas, salud pública BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA MICROBIOLOGÍA MICROBIOLOGÍA CLÍNICA MICROBIOLOGÍA CLÍNICA

CRECIMIENTO Y SUPERVIVENCIA DEL LANGOSTINO (Macrobrachium tenellum) EN CULTIVOS EXPERIMENTALES DE VERANO Y OTOÑO EN LA COSTA TROPICAL DEL PACÍFICO MEXICANO

GROWTH AND SURVIVAL OF PRAWN (Macrobrachium tenellum) IN EXPERIMENTAL CULTURES DURING SUMMER AND AUTUMN IN THE TROPICAL MEXICAN PACIFIC COAST

FERNANDO VEGA VILLASANTE Luis Daniel Espinosa Chaurand Héctor Gerardo Nolasco Soria (2011)

"Para fines de cultivo, Macrobrachium tenellum es considerado como un buen candidato pues no es agresivo ni presenta canibalismo, puede tolerar un amplio rango de temperaturas, salinidades y concentraciones de oxígeno. El presente trabajo evalúa el cultivo semiintensivo de M. tenellum bajo condiciones ambientales de verano y otoño con especial atención en la temperatura del agua. Los resultados de los cultivos experimentales en la costa tropical del Pacífico mexicano, sugieren que esta especie demuestra mejor crecimiento durante el final de la primavera, verano y principios del otoño, época en que la temperatura promedio del agua es cercana a 30°C. Mientras que los cultivos de finales de otoño e inicios de invierno demuestran crecimientos mínimos, con una temperatura promedio del agua de cultivo de 27°C. Otros parámetros físicoquímicos como pH, concentración de O2 y turbidez en el agua de cultivo fueron similares en todos los cultivos experimentales por lo que se sugiere que el factor temperatura fue el determinante en las diferencias encontradas en el crecimiento."

"For aquaculture purposes, Macrobrachium tenellum is considered as a good candidate, is not aggressive nor presents cannibalism and can tolerate an ample interval of temperatures, salinities and oxygen concentrations. The present work evaluates the semi-intensive culture of M. tenellum under environmental conditions of summer and autumn with special attention to water temperature. The results of the experimental cultures in the tropical Mexican Pacific coast, suggest this species demonstrates better growth during the end of the spring, summer and the beginning of the autumn, time at which the average temperature of the water is near 30°C. The experimental cultures of end of autumn and beginnings of winter demonstrate minimum growth, with an average temperature of the culture water of 27°C. Other parameters like pH, O2 concentration and turbidity in the culture water were similar in all the experimental cultures reason why temperature is suggested the factor was the determinant in the differences found in growth."

Article

camarón de río, temperatura, desarrollo, Macrobrachium, condiciones ambientales prawn, temperature, development, environmental conditions BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA BIOLOGÍA ANIMAL (ZOOLOGÍA) CRECIMIENTO ANIMAL CRECIMIENTO ANIMAL

Capacidad depredadora del langostino Macrobrachium tenellum sobre larvas de Aedes aegypti en condiciones de laboratorio

Predatory capacity of Macrobrachium tenellum on Aedes aegypti larvae in lab conditions

MANUEL ALEJANDRO VARGAS CEBALLOS LUIS EDUARDO RUIZ GONZALEZ LUIS DANIEL ESPINOSA CHAURAND HECTOR GERARDO NOLASCO SORIA FERNANDO VEGA VILLASANTE (2012)

"Introducción: en los últimos años se le ha dado gran importancia a los depredadores naturales de Aedes aegypti. Se han investigado, tanto en el campo como en el laboratorio, los distintos organismos con capacidad de devorar larvas de este mosquito. Macrobrachium tenellum se encuentra en altas densidades en condiciones naturales, no es agresivo y puede tolerar un amplio y fluctuante intervalo de temperaturas, salinidades y concentraciones de oxígeno. Objetivo: evaluar la capacidad depredadora de Macrobrachium tenellum sobre larvas de Aedes aegypti en condiciones de laboratorio. Métodos: se utilizaron juveniles de Macrobrachium tenellum con dos rangos de tallas: A (3,0 cm-3,5 cm) y B (4,5 cm-5,0 cm). Las larvas del mosquito se obtuvieron de la eclosión de huevos producidos por hembras adultas mantenidas en una jaula entomológica. En el primer bioensayo se colocaron 5, 10, 15 y 20 larvas de Aedes aegypti por tratamiento por rango. En el segundo bioensayo el número de larvas se ajustó a 30, 40, 50 y 80 larvas por tratamiento por rango. Resultados: Macrobrachium tenellum demostró un alto consumo de larvas para los dos rangos y tratamientos. En la mayor densidad (80 larvas) se obtuvo el consumo de 95 % de larvas a las 24 h para el rango A y 100 % para el rango B. Conclusión: Macrobrachium tenellum puede ser considerado como un potencial agente de control biológico debido a su abundancia en ambientes naturales, su resistencia a diversas condiciones ambientales y a la voracidad presentada en este estudio."

"Introduction: in the last few years, a lot of importance has been given to natural predators against Aedes aegypti. Several organisms have been studied both in lab and in the field so as to find out their capacity to devour mosquito larvae. High densities of Macrobrachium tenellum are found in natural conditions, it is not aggressive and may stand wide ranges of temperature, rates of salinity and oxygen concentrations. Objective: to evaluate the predatory capacity of Macrobrachium tenellum on Aedes aegypti larvae in lab conditions. Methods: very young Macrobrachium tenellum prawns measuring A(3.0-3.5cm) and B(4.5-5 cm) were used. The mosquito larvae were obtained after hatching of egss from adult females kept in entomological cages. Five, ten, fifteen and twenty Aedes aegypti larvae were placed per treatment per rank, whereas the second bioassays adjusted the number of larvae to 30, 40, 50 and 80 larvae per treatment per rank. Results: Macrobrachium tenellum showed high rate of larval consumption for the two ranks and treatments. In the highest density (80 larvae), the consumption was 95% of larvae at 24 hours for rank A and 100% for rank B. Conclusions: Macrobrachium tenellum may be considered as a potential biological control agent, due to its abundant presence in natural conditions, its resistance to different environmental conditions and to its voraciousness seen in this study."

Article

Aedes aegypti, Macrobrachium tenellum, larvas, capacidad depredadora, control biológico larvae, predatory capacity, biological control BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA BIOLOGÍA ANIMAL (ZOOLOGÍA) COMPORTAMIENTO ANIMAL COMPORTAMIENTO ANIMAL

Effect of feeding frequency on growth and survival in juvenile gar Atractosteus tropicus Gill, 1863, in culture conditions

Leonardo Martínez Cárdenas Crisantema Hernández González Luis Daniel Espinosa Chaurand MIRIAM SORIA BARRETO Carlos Alfonso Álvarez González rafael martinez-garcia (2018)

"The gar, Atractosteus tropicus is a native fish distributed in fluvial systems of the Mexican southeast, which presents aquaculture potential in the food industry and as ornamental species. However, it is necessary to deepen the knowledge for the optimization of its culture. The present study examined the effect of three feeding frequencies on the growth, survival, and condition of juveniles. The fish (90) were placed in nine 35 L tanks, (n = 10; three replicates per treatment). The feed (44% protein, 15% fat) was supplied to 2% of the biomass, divided into 2, 4 and 6 servings throughout 10 h for eight weeks. After eight weeks the standard length, wet weight, survival, Fulton K, specific growth rate, nitrogen carbon rate, the coefficient of variation and size heterogeneity were recorded, which were analyzed with a one-way ANOVA. There were no significant differences among treatments for any of the response variables. The overall results indicated that in general both the culture conditions and the three food frequencies tested were adequate for the species. The results indicate the high flexibility of the species to be cultured with minimum requirements of the food supply, which can translate into the optimization of production costs."

Article

aquarium, pellets, condition, carbon, nitrogen rate, moisture content, aquaculture BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA BIOLOGÍA ANIMAL (ZOOLOGÍA) NUTRICIÓN NUTRICIÓN