Título
Environmental surveillance of SARS-CoV-2 RNA in wastewater and groundwater in Quintana Roo, Mexico
Autor
GABRIELA ROSILES GONZALEZ
VICTOR HUGO CARRILLO JOVEL
Liliana María Alzate Gaviria
Walter Betancourt
Charles Gerba
OSCAR ALBERTO MORENO VALENZUELA
RAUL TAPIA TUSSELL
Cecilia Hernández Zepeda
Nivel de Acceso
Acceso Abierto
Referencia de datos
datasetDOI/https://doi.org/10.1007/s12560-021-09492-y
Materias
Resumen o descripción
The presence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA in wastewater has been reported as a result of fecal shedding of infected individuals. In this study, the occurrence of SARS-CoV-2 RNA was explored in primary-treated wastewater from two municipal wastewater treatment plants in Quintana Roo, Mexico, along with groundwater from sinkholes, a household well, and submarine groundwater discharges. Physicochemical variables were obtained in situ, and coliphage densities were determined. Three virus concentration methods based on adsorption-elution and sequential filtration were used followed by RNA isolation. Quantification of SARS-CoV-2 was done by RT-qPCR using the CDC 2020 assay, 2019-nCoV_N1 and 2019-nCoV_N2. The Pepper mild mottle virus, one of the most abundant RNA viruses in wastewater was quantified by RT-qPCR and compared to SARS-CoV-2 concentrations. The use of three combined virus concentration methods together with two qPCR assays allowed the detection of SARS-CoV-2 RNA in 58% of the wastewater samples analyzed, whereas none of the groundwater samples were positive for SARS-CoV-2 RNA. Concentrations of SARS-CoV-2 in wastewater were from 1.8 × 103 to 7.5 × 103 genome copies per liter (GC l−1), using the N1 RT-qPCR assay, and from 2.4 × 102 to 5.9 × 103 GC l−1 using the N2 RT-qPCR assay. Based on PMMoV prevalence detected in all wastewater and groundwater samples tested, the three viral concentration methods used could be successfully applied for SARS-CoV-2 RNA detection in further studies. This study represents the first detection of SARS-CoV-2 RNA in wastewater in southeast Mexico and provides a baseline for developing a wastewater-based epidemiology approach in the area.
Fecha de publicación
2021
Tipo de publicación
Artículo
Versión de la publicación
Versión publicada
Recurso de información
Formato
application/pdf
Fuente
Food and Environmental Virology, 13(4), 457-469, 2021.
Idioma
Inglés
Relación
&
Hernández-Zepeda, C. (2021). Environmental surveillance of SARS-CoV-2 RNA in wastewater and groundwater in Quintana Roo, Mexico. Food and Environmental Virology, 13(4), 457-469.
Sugerencia de citación
Rosiles-González, G., Carrillo-Jovel, V. H., Alzate-Gaviria, L., Betancourt, W. Q., Gerba, C. P., Moreno-Valenzuela, O. A., ...
Repositorio Orígen
Repositorio Institucional CICY
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