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Selecciona los temas de tu interés y recibe en tu correo las publicaciones más actuales
An efficient transformation method for genome editing of elite bread wheat cultivars
Akshaya Biswal Kanwarpal Dhugga (2023, [Artículo])
Particle Bombardment Wheat Growth-Regulating Factor 4 Wheat Growth-Interacting Factor Wheat Mildew Locus O CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA CRISPR GENE EDITING TRANSFORMATION WHEAT
Synthetic libraries of shark vNAR domains with different cysteine numbers within the CDR3
OLIVIA CABANILLAS BERNAL (2019, [Artículo])
The variable domain of New Antigen Receptors (vNAR) from sharks, present special characteristics in comparison to the conventional antibody molecules such as: small size (12–15 kDa), thermal and chemical stability and great tissue penetration, that makes them a good alternative source as therapeutic or diagnostic agents. Therefore, it is essential to improve techniques used for the development and selection of vNAR antibodies that recognize distinct antigens. The development of synthetic antibody libraries offers a fast option for the generation of antibodies with the desired characteristics. In this work three synthetic antibody libraries were constructed; without cysteines (Cys), with one Cys and with two Cys residues within its CDR3, with the objective of determining whether the presence or absence of Cys in the CDR3 favors the isolation of vNAR clones from a synthetic library. The libraries were validated selecting against six mammalian proteins. At least one vNAR was found for each of the antigens, and a clone coming from the library without Cys in the CDR3 was selected with all the antigens. In vitro angiogenesis assay with the isolated anti-VEGF antibodies, suggest that these vNARs are capable of inhibiting in vitro angiogenesis. In silico analysis of anti-VEGF antibodies showed that vNARs from synthetic libraries could rival antibodies with affinity maturation by in silico modeling. © 2019 Cabanillas-Bernal et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
aquaporin 1, carcinoembryonic antigen, cysteine, fibroblast growth factor 2, glycophorin A, leukemia inhibitory factor, vasculotropin, vasculotropin antibody, angiogenesis inhibitor, antibody, cysteine, lymphocyte antigen receptor, vasculotropin A, a BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA BIOFÍSICA BIOFÍSICA
Akshaya Biswal Daisuke Urano (2022, [Artículo])
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Carlo Montes Anton Urfels Eunjin Han Balwinder-Singh (2023, [Artículo])
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Gerald Blasch (2023, [Objeto de congreso])
CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA REMOTE SENSING WHEAT CROPS DISEASES
Leonardo Abdiel Crespo Herrera (2022, [Objeto de congreso])
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Comprehending the evolution of gene editing platforms for crop trait improvement
deepmala sehgal Apekshita Singh SoomNath Raina (2022, [Artículo])
Cas9 Base Editing Prime Editing Epigenome Editing CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA CRISPR ABIOTIC STRESS ARABIDOPSIS CROP IMPROVEMENT DNA ELECTROPORATION GENE EDITING RICE WHEAT
Trade-offs in the genetic control of functional and nutritional quality traits in UK winter wheat
Nick Fradgley Keith Gardner Stéphanie M. Swarbreck Alison Bentley (2022, [Artículo])
CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA WHEAT GENETIC CONTROL NUTRITIVE VALUE QUANTITATIVE TRAIT LOCI
Digital artifacts reveal development and diffusion of climate research
Bia Carneiro Tek Sapkota (2022, [Artículo])
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