Agricultural value chains in Sudan: an annotated bibliography

Hugo De Groote Paswel Marenya (2023, [Libro])

CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA AGRICULTURAL VALUE CHAINS CASH CROPS FOOD CROPS LIVESTOCK SOCIOECONOMIC ASPECTS GENDER SOCIAL INCLUSION POSTHARVEST LOSSES BIBLIOGRAPHIES

A twelve years story of waste of social resources: The Mexico-Toluca Interurban Train

Rubén Chavarín (2024, [Artículo, Artículo])

The general objective of this paper is to make an ex-post evaluation of the Mexico-Toluca Interurban Train project (TIMT) more than nine years after the beginning of its construction stage. The present study is based on the cost-benefit analysis approach, through which some analysis scenarios have been built and whose results are contrasted with the official project evaluations. The present research has focused on the analysis of the TIMT as a microcosm of the scarce efficiency of public investment in Mexico, resulting in a waste of social resources. According to the results of the study, the combination of delays and cost overruns (186% in nominal terms, 114% in real terms, and 90% in dollars) has configured a project (across two Federal Government Administrations) with negative social profitability, with losses valued at more than 550 million dollars, placing it in the most problematic quartile of train projects in the world.

cost-benefit analysis ex-post evaluation public investment transport projects Latin America análisis costo-beneficio evaluación ex-post inversión pública proyectos de transporte América Latina CIENCIAS SOCIALES CIENCIAS SOCIALES

Gráfica de protesta: PuntosB, Lapiztola y La Guillotina. Un análisis entre el cuerpo y el espacio en la imagen de protesta

Jorge Gabriel Ortiz Leroux (2023, [Capítulo de libro])

Capítulo número 5 de la Sección Imágenes y representaciones.

El objetivo del presente texto es el de mostrar como la obra emanada de colectivos de análisis del espacio social de la protesta, permite reconocer las claves que relacionan al espacio con los actores que lo ocupan. Asimismo, se pretende abordar cómo el conflicto que se interpone entre los actores de la protesta define sus magnitudes y reelabora sus disposiciones y sus formas de entrecruzamiento en relación con la presencia de la imagen. Asimismo, la representación imaginaria y visual del espacio permite ver a la ciudad como un laboratorio, que puede también ser registrado, interpretado y resituado respecto a los discursos hegemónicos. Los proyectos visuales que aquí abordamos representan un trabajo que a través de los años ha expresado su visión sobre las realidades sociales y políticas de nuestro país, en las que el conflicto aparece como telón de fondo de la creación e interpretación visual de esas realidades. La interacción entre política y estética tiene como escenario el espacio público y la ciudad, que fungen como trasfondo para la expresión de formas imaginarias y que conforman en sí mismas el sentido de los reclamos y las transformaciones gestadas. Mirando a la ciudad, observamos sus cambios y las formas de relación, cada vez más complejas, que se viven en ella.

Public spaces--Social aspects--Mexico. Dissident art. Protest movements--Mexico--Posters. Graphic arts--Political aspects--Mexico. Political posters, Mexican--20th century. Espacios públicos -- México. Arte disidente. Movimientos de protesta. Carteles políticos mexicanos. HT185 HUMANIDADES Y CIENCIAS DE LA CONDUCTA CIENCIAS DE LAS ARTES Y LAS LETRAS TEORÍA, ANÁLISIS Y CRÍTICA DE LAS BELLAS ARTES

Potential of Debaryomyces hansenii Strains on the Inhibition of Botrytis cinerea in Blueberry Fruits (Vaccinium corymbosum L.)

SURELYS RAMOS BELL Luis Guillermo Hernández Montiel RITA MARIA VELAZQUEZ ESTRADA JUAN ANTONIO HERRERA GONZALEZ PORFIRIO GUTIERREZ MARTINEZ (2022, [Artículo])

"Blueberry (Vaccinium corymbosum L.) is a crop with great potential for exportation and is very important for its antioxidant properties. However, this fruit is susceptible to different factors of deterioration and attack by pathogens. Botrytis cinerea is the main cause of post-harvest losses in this crop. Antagonistic yeasts from the Debaryomyces hansenii species, which is of marine origin, have been proposed as an alternative method for fungal control. For this reason, this study evaluated the in vitro and in vivo antagonistic capacity of the yeast against B. cinerea. The in vitro growth of the fungus was inhibited by 90% and germination was reduced by 100%, and through the production of volatile organic compounds, the growth of the pathogen was inhibited by 32.5%. Photographic evidence by electron microscopy revealed the effects caused by D. hansenii on B. cinerea. The incidence study showed a 50% reduction in the incidence of the disease caused by B. cinerea in blueberries, while quality parameters—such as soluble solids, titratable acidity, and pH—presented stable values, delaying the maturation process of the fruits. Likewise, the rate of reduction in firmness and physiological weight loss was lower, and the blueberries preserved their quality for a longer time."

Debaryomyces hansenii, blueberry, gray mold, postharvest quality CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA CIENCIAS AGRARIAS FITOPATOLOGÍA FITOPATOLOGÍA FITOPATOLOGÍA

Aplicación de 1-MCP en diferentes ecotipos de ciruela mexicana (Spondias purpurea L.) de México

MARÍA GORETTI TRANSITO DAMASO (2023, [Tesis de maestría])

La ciruela mexicana es un frutal nativo de México, se distribuye en la vertiente del

Pacífico, centro de Veracruz y Península de Yucatán. El árbol de ciruela mexicana

produce frutos de diferentes tamaños, colores y sabores, existe una gran diversidad

que se aprecia tanto en los meses de marzo a junio, como de septiembre a noviembre.

El principal uso de la fruta es en fresco como fruta de temporada, la vida útil de la

ciruela de estación seca no es mayor a 5 d, por lo cual es un frutal que se considera

importante de manera local. Actualmente poco se han desarrollado y evaluado

tecnologías para incrementar la vida útil de este producto hortícola, por lo que en el

presente trabajo se evaluó la respuesta de cinco ecotipos de ciruela mexicana a la

aplicación de 1-metilciclopropeno (1-MCP), con la finalidad de proponerlo para el

desarrollo del manejo poscosecha adecuado de esta fruta. Durante abril a septiembre

de 2022 se cosecharon frutos de ciruela mexicana procedentes de Guerrero y Morelos.

Los frutos se cosecharon en etapa ½ verde. Los frutos fueron transportados al

Laboratorio de Producción Agrícola de la Facultad de Ciencias Agropecuarias en la

Universidad Autónoma del Estado de Morelos, donde se lavaron y se dejaron secar al

ambiente. Posteriormente se colocaron en cajas de plástico herméticas durante 12 h

y se aplicó 0, 500 y 1000 nL L-1 de 1-MCP, se evaluaron a temperatura ambiente (24.9

± 1 °C; 55.1 ± 6 % HR) y después de 5, 10 y 15 d en almacenamiento a 12 °C. Se

evaluaron los cambios fisicoquímicos y fisiológicos de cada ecotipo. La aplicación de

1-MCP, independiente del tiempo de almacenamiento inhibió y retrasó la velocidad de

respiración y producción de etileno, mantuvo la firmeza y retrasó la pigmentación

característica de cada ecotipo, con poca acción en la concentración de sólidos solubles

totales y acidez titulable, sin efecto claro en compuestos fenólicos, flavonoides y

actividad antioxidante, y sin ningún efecto en la pérdida de masa. El efecto del 1-MCP

permite conservar la fruta a temperatura ambiente hasta 6 u 8 d; la refrigeración a 12

°C potencia la acción del 1-MCP permitiendo conservar hasta 9 a 20 d después de

salir de almacenamiento.

The Mexican plum is a fruit native to Mexico, it is distributed on the Pacific slope, central

Veracruz and Yucatan Peninsula. The Mexican plum tree produces fruits of different

sizes, colors and flavors, there is a great diversity that is appreciated both in the months

of March to June, and from September to November. The main use of the fruit is fresh

as a seasonal fruit, the shelf life of the dry season plum is not more than 5 d, so it is a

fruit tree that is considered important locally. Currently, little technologies have been

developed and evaluated to increase the shelf life of this horticultural product, so in this

work the response of five ecotypes of Mexican plum to the application of 1-

methylcyclopropene (1-MCP) was evaluated, in order to propose it for the development

of adequate postharvest management of this fruit. During April to September 2022,

Mexican plum fruits were harvested from Guerrero and Morelos. The fruits were

harvested in stage 1/2 green. The fruits were transported to the Agricultural Production

Laboratory of the Faculty of Agricultural Sciences at the Autonomous University of the

State of Morelos, where they were washed and left to dry in the environment.

Subsequently, they were placed in airtight plastic boxes for 12 h and 0, 500 and 1000

nL L-1 of 1-MCP were applied, evaluated at room temperature (24.9 ± 1.1 °C; 55.1 ±

6.2 % RH) and after 5, 10 and 15 d in storage at 12 °C. The physicochemical and

physiological changes of each ecotype were evaluated. The application of 1-MCP,

independent of storage time inhibited and delayed the rate of respiration and production

of ethylene, maintained the firmness and delayed the pigmentation characteristic of

each ecotype, with little action on the concentration of total soluble solids and titratable

acidity, with no clear effect on phenolic compounds, flavonoids and antioxidant activity,

and without any effect on mass loss. The effect of 1-MCP allows the fruit to be

preserved at room temperature up to 6 or 8 d; cooling at 12 °C enhances the action of

1-MCP allowing to preserve up to 9 to 20 d after leaving storage

CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA CIENCIAS AGRARIAS Ciruela mexicana, 1-metilciclopropeno, almacenamiento, refrigeración, calidad, poscosecha Mexican plum, 1-methylcyclopropene, storage, refrigeration, quality, postharvest.

Evaluación del uso de vibriófagos encapsulados en el alimento para el control de Vibrio parahaemolitycus causante de AHPND en el camarón Penaeus vannamei

Esther Imelda Ponce García (2023, [Tesis de maestría])

"Las enfermedades causadas por bacterias del género Vibrio en el cultivo de camarón son de gran importancia debido a su impacto negativo en la industria acuícola. Estas bacterias pueden causar diversas enfermedades en camarones, como la necrosis hepatopancreática aguda (AHPND). La fagoterapia es una alternativa para el biocontrol de estas bacterias patógenas que presenta importantes ventajas, como la alta especificidad a sus huéspedes, la replicación en el sitio de infección, y la capacidad de infectar a bacterias resistentes a antibióticos. Sin embargo, la estabilidad de los fagos en el sistema de cultivo es una preocupación latente, ya que los fagos presentan una estabilidad limitada en solución y experimentan una caída significativa en el título de fagos durante el procesamiento y almacenamiento. El método de encapsulación de bacteriófagos, un área que aún no se estudia del todo, se presenta como una potencial alternativa para atender esta problemática. En el presente estudio se evaluó la eficacia terapéutica de un cóctel de bacteriófagos (vB_Vp_PvVp05, vB_Vp_PvVp07 y vB_Vp_PvVp11), encapsulados en alginato de sodio comercial y de mediana viscosidad, pectina, carboxilmetilcelulosa, liposomas y liofilizado en pectina como agente de biocontrol de Vibrio parahaemolyticus para su uso en terapias fagicas pasiva y activa. Se observó que el encapsulado en alginato de sodio de mediana viscosidad presentó la mayor liberación de fagos activos y al momento de ser incorporado a alimento para camarón y horneados a 80 – 100°C, se mantuvo una concentración de 1.3 x105 UFP/g después de 100 días de almacenamiento. Para la evaluación de las terapias pasiva y activa se realizó una infección experimental en juveniles de Penaeus vannamei para conocer la efectividad de los encapsulados, utilizando para la terapia pasiva la cepa Vp M0904 y para la activa la cepa Vp M0605. El experimento de terapia pasiva mostró una mortalidad del 80.6% en los camarones a las 48 hpi (p > 0.05) y una concentración de 5 x102 UFC/mL de Vibrio spp. no fermentativos en TCBS. En el caso de la terapia activa no se presentó mortalidades, pero hubo una disminución de la concentración de Vibrio spp. no fermentativos en TCBS con 5.56 x101 UFC/mL a las 48 hpi (p < 0.05) y aumentó la densidad de vibriófagos en 1.4 x102 UFP/mL en el agua de los acuarios (p < 0.05)..."

"Diseases caused by bacteria of the Vibrio genus in shrimp farming are of great importance due to their negative impact on the aquaculture industry. These bacteria can cause various diseases in shrimp, such as acute hepatopancreatic necrosis disease (AHPND). Phage therapy is an alternative for the biocontrol of these pathogenic bacteria, offering significant advantages, including high specificity to their hosts, replication at the infection site, and the ability to infect antibiotic-resistant bacteria. However, the stability of phages in the farming system is a persistent concern, as phages have limited stability in solution and undergo a significant drop in phage titer during processing and storage. The method of bacteriophage encapsulation, an area that is not yet fully explored, emerges as a potential alternative to address this issue. In this study, the therapeutic efficacy of a cocktail of bacteriophages (vB_Vp_PvVp05, vB_Vp_PvVp07, and vB_Vp_PvVp11), encapsulated in commercial medium-viscosity sodium alginate, pectin, carboxymethylcellulose, liposomes, and lyophilized pectin, was evaluated as a biocontrol agent against Vibrio parahaemolyticus for use in passive and active phage therapies. It was observed that encapsulation in medium-viscosity sodium alginate showed the highest release of active phages, maintaining a concentration of 1.3 x 105 PFU/g after 100 days of storage when incorporated into shrimp feed and baked at 80–100°C.For the evaluation of passive and active therapies, an experimental infection was conducted on Penaeus vannamei juveniles to assess the effectiveness of the encapsulated phages. The passive therapy experiment showed a mortality rate of 80.6% in shrimp at 48 hpi (p > 0.05) and a concentration of 5 x 102 CFU/mL of non-fermentative Vibrio spp. on TCBS agar. In the case of active therapy, no mortalities were observed, but there was a decrease in the concentration of non-fermentative Vibrio sp. on TCBS agar to 5.56 x 101 CFU/mL at 48 hpi (p < 0.05), and the density of vibriophages increased to 1.4 x 102 PFU/mL in the aquarium water (p < 0.05). It was determined that passive therapy offered no advantage for the control of Vibrio parahaemolyticus, unlike active therapy, which proved to be effective in controlling this bacterium."

vibriófagos, encapsulado, Penaeus vannamei, terapia activa, terapia pasiva vibriophages, encapsulated, active therapy, passive therapy BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA MICROBIOLOGÍA BACTERIÓFAGOS BACTERIÓFAGOS

How a holobiome perspective could promote intensification, biosecurity and eco-efficiency in the shrimp aquaculture industry

Eric Daniel Gutiérrez Pérez RICARDO VAZQUEZ JUAREZ FRANCISCO JAVIER MAGALLON BARAJAS MIGUEL ANGEL MARTINEZ MERCADO GRISEL ALEJANDRA ESCOBAR ZEPEDA Paola Magallón Servín (2022, [Artículo])

"The aquaculture industry faces many challenges regarding the intensification of shrimp rearing systems. One of these challenges is the release of excessive amounts of nitrogen and phosphorus into coastal areas, causing disruption in nutrient cycling and microbial equilibrium, which are important for coastal productivity. Biosecurity within the shrimp rearing systems can also be compromised by disruption to the nutrient fluxes, and as consequence the microbiome of the system. In certain conditions, these changes could lead to the blooming of potentially pathogenic bacteria. These changes in the external microbiome of the system and the constant fluctuations of nutrients can affect the intestinal microbiome of shrimp, which is involved in the growth and development of the host, affecting nutrient absorption, regulating metabolic processes, synthesising vitamins, modulating the immune response and preventing growth of pathogenic bacteria. It has been suggested that specific changes in the intestinal microbiome of Litopenaeus vannamei may be an avenue through which to overcome some of the problems that this industry faces, in terms of health, growth and waste. Recent research, however, has focussed mainly on changes in the intestinal microbiome. Researchers have overlooked the relevance of other aspects of the system, such as the microbiome from the benthic biofilms; zooplankton, plankton and bacterioplankton; and other sources of microorganisms that can directly affect the microbial status of the intestinal and epiphytic communities, especially in rearing systems that are based on intensification and microbial maturation processes, such as a biofloc system. It is therefore necessary to place holobiome studies into context, including the ‘holobiome of the aquaculture system’ (microbiomes that make up the culture system and their interactions) and not only the intestinal microbiome. Thus, we describe factors that affect the shrimp microbiome, the methodology of study, from sampling to bioinformatic workflows, and introduce the concept of the ‘holobiome of the aquaculture system’ and how this enables us to promote the intensification, biosafety and eco-efficiency of shrimp farming. The holobiome perspective implies a greater investment of resources and time for research, but it will accelerate the development of technology that will benefit the development and sustainability of the aquaculture industry."

litopenaeus vannamei, microbiome, intensification, biofloc, holobiome of aquaculture systems CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA CIENCIAS AGRARIAS PRODUCCIÓN ANIMAL NUTRICIÓN NUTRICIÓN

Gene expression in primary hemocyte culture of the Pacific white shrimp Penaeus vannamei infected with different white spot syndrome virus (WSSV) strains

Delia Patricia Parrilla Taylor REGINA ELIZONDO GONZALEZ Jesús Neftalí Gutiérrez Rivera SILVIA ALEJANDRA GARCIA GASCA NORBERTO VIBANCO PEREZ MA. DE JESUS DURAN AVELAR RICARDO VAZQUEZ JUAREZ (2022, [Artículo])

"Five previously analyzed white spot syndrome virus (WSSV) strains from northwest Mexico,differing in their genome architecture as well as in virulence, were selected (high virulence JP and LG strains;moderate virulence GVE and DIV strains; and low virulence LC10 strain) to evaluate pathogenesis response in vitro. Expression of phagocytosis-activating protein PAP, manganese superoxide dismutase MnSOD and peroxiredoxin PRX, and two genes of immediate-early expression (IE1 and WSSV304) were measured by qPCR in a primary hemocyte cell culture from Penaeus vannamei at 1, 3, 6, 12, and 24 h post-infection (hpi). PAP expression was significantly higher at 1 and 3 hpi, and JP and LC10 strains induced the highest expression. The response of MnSOD was high at 1 hpi, and a significant increase in PRX expression was detected at 3 hpi, probably due to the occurrence of an oxidative burst; expression levels of MnSOD and PRX were significantly higher at 1 and 3 hpi, respectively, induced by the LG strain (high virulence), suggesting an acute response. In general, expression of most immune-related - genes decreased after the initial hours of infection. Expression levels of IE1 and WSSV304 were exceptionally high at 1 hpi in almost all five WSSV analyzed strains,confirming their efficient mechanism for replication and viral fitness. The results of this study do not show an accurate link between the genome size and WSSV virulence of the strains, albeit the strain with the smallest genome showed the highest virulence. All strains induced an early immune response in heterogeneous ways."

Penaeus vannamei, virulence, gene expression, viral fitness, viral pathogenesis, immune response BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA BIOLOGÍA ANIMAL (ZOOLOGÍA) PATOLOGÍA ANIMAL PATOLOGÍA ANIMAL

Uso del cártamo (Carthamus tinctorius L.) como ingrediente en alimentos para juveniles del camarón Litopenaeus vannamei

ALFONSO GALICIA GONZALEZ (2009, [Tesis de doctorado])

Debido a los altos costos e incertidumbre en el abasto de la harina de pescado y pasta de soya, en los últimos años se han buscado alternativas que puedan sustituirlos sin tener un efecto negativo en el crecimiento de los camarones en el cultivo. En el presente trabajo se realizó un estudio para evaluar el valor nutricional del cártamo (Carthamus tinctorius) como fuente proteica en alimentos para juveniles de camarón blanco del Pacífico Litopenaeus vannamei. El primer capítulo comprende el estudio de la caracterización de tres productos de cártamo (HIC; Harina Integral de Cártamo, PCB; Pasta de cártamo baja en proteína y PCA; Pasta de cártamo alta en proteína) en base a su composición de nutrientes y algunos factores antinutricionales (hemaglutininas, saponinas, actividad uréasica, aflatoxinas e inhibidor de tripsina). Los productos de cártamo tuvieron un contenido de proteína entre 20.6 y 36.8%. La harina integral fue la que presentó el mayor nivel de extracto etéreo 31%, mientras que las pastas tuvieron un nivel bajo (1.8-1.0%). El contenido de fibra en el cártamo fue alto (17-23%). Los aminoácidos más abundantes en los productos de cártamo fueron el ácido aspártico y glutámico, en contraste los menos abundantes fueron lisina y metionina. Los ácidos grasos más abundantes fueron el ácido oleico, el linoleico, el palmítico y el estéarico. El contenido de calcio de los productos de cártamo varió de 0.25 a 0.42%, mientras que el contenido de fósforo varió del 0.23 a 0.25%. No se encontraron los factores antinutricionales buscados, a excepción del inhibidor de tripsina que tuvo valores bajos (7.56 UTI/mg de muestra). No se encontraron diferencias significativas en los coeficientes de utilización digestiva aparente (CUDA) de materia seca y carbohidratos de los productos de cártamo; sin embargo, sí se encontraron diferencias en los CUDA de proteína, lípidos (CUDAl) y de energía digestible (CUDAe). La actividad de las enzimas digestivas de camarones que fueron alimentados con productos de cártamo mostró un incremento en proteinasas generales y quimotripsina. La atractabilidad y el consumo de los alimentos con un 30% de inclusión de los productos de cártamo se vieron afectadas negativamente en los camarones [...]

In recent years, alternatives to fish and soybean meals in aquacultural feeds that do not have negative effects on growth of cultivated shrimp have been intensively studied in an effort to reduce the high costs and uncertainty in supplies. In this study, the nutritional value of safflower (Carthamus tinctorius) meal as a protein source in diets for juveniles Pacific whiteleg shrimp (Litopenaeus vannamei) was investigated. Initially, characterization of three safflower meals (i.e. WSM; whole safflower meal, LPSM; low-protein safflower meal and HPSM; high-protein safflower meal), focusing on chemical composition and antinutritional factors (hemaglutinine, saponine, ureasic activity, aflatoxin, and trypsin inhibitor) was conducted. Protein content ranged from 20.6 to 36.8%. Whole safflower meal contained the highest level of lipids (31%); lipids in the other safflower meals were as low as 1.0–1.8%. Fiber content was high (17–23%). The most abundant amino acids were aspartic and glutamic acids and lysine and methionine were less abundant. Oleic, linoleic, palmitic, and stearic fatty acids were present in greatest amounts. Calcium content ranged from 0.25–0.42% and phosphorus content ranged from 0.23–0.25%. Anti-nutritional factors were not found in the analyses, except for trypsin inhibitor, with values as low as 7.56 UTI mg–1 per sample. No significant differences between the apparent digestibility coefficients (ADC) for dry matter and carbohydrates in safflower meals were detected; however, protein ADC, lipid ADC, and energy ADC were different. Total proteinase and chymotrypsin increased significantly in diets where safflower meals were included. Atractability and consumption of feed with 30% safflower meals appeared to have negative effects on the juveniles [...]

atractabilidad; camarón; cártamo; Carthamus tinctorius; digestibilidad; enzimas digestivas; factores antinutricionales; Litopenaeus vannamei CIENCIAS FÍSICO MATEMÁTICAS Y CIENCIAS DE LA TIERRA CIENCIAS DE LA TIERRA Y DEL ESPACIO OCEANOGRAFÍA OCEANOGRAFÍA ACUICULTURA MARINA OCEANOGRAFÍA ACUICULTURA MARINA

Chronic toxicity of shrimp feed added with silver nanoparticles (Argovit-4®) in Litopenaeus vannamei and immune response to white spot syndrome virus infection

Carlos Rosendo Romo Quiñonez Píndaro Álvarez Ruiz CLAUDIO HUMBERTO MEJIA RUIZ NINA BOGDANCHIKOVA Alexey Pestryakov CARINA GAMEZ JIMENEZ WENCESLAO VALENZUELA QUIÑONEZ Magnolia Montoya Mejía EUSEBIO NAVA PEREZ (2022, [Artículo])

"In recent years, the application of silver nanoparticles (AgNPs) as antibacterial compounds has been widely used in human and veterinary medicine. In this work, we investigated the effects of AgNPs (Argovit-4 R ) as feed additives (feed-AgNPs) on shrimp (Litopenaeus vannamei) using three different methods: 1) chronic toxicity after 28 days of feeding, 2) Effects against white spot syndrome virus (WSSV) challenged by oral route, and 3) transcriptional responses of immune-related genes (PAP, ProPO, CTL-3, Crustin, PEN3, and PEN4) following WSSV infection. The results showed that the feed-AgNPs did not interfere with the growth and survival of shrimp. Also, mild lesions in the hepatopancreas were recorded, proportional to the frequency of the feed-AgNP supply. Challenge test versus WSSV showed that feeding every 7 days with feed-AgNPs reduced mortality, reaching a survival rate of 53%, compared to the survival rates observed in groups fed every 4 days, daily and control groups of feed-AgNPs for the 30%, 10%, and 7% groups, respectively. Feed-AgNPs negatively regulated the expression of PAP, ProPO, and Crustin genes after 28 days of treatment and altered the transcriptional responses of PAP, ProPO, CTL-3, and Crustin after WSSV exposure. The results showed that weekly feeding-AgNPs could partially prevent WSSV infection in shrimp culture. However, whether or not transcriptional responses against pathogens are advantageous remains to be elucidated."

Silver nanoparticles, Shrimp, Aquaculture, Chronic toxicity, WSSV, AgNP, Argovit, Litopenaeus vannamei , Silver fed, White spot syndrome virus BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA INMUNOLOGÍA INMUNIZACIÓN INMUNIZACIÓN