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José Antonio Cruz-Barraza (2012, [Artículo])
Integrative taxonomy provides a major approximation to species delimitation based on integration of different perspectives (e.g. morphology, biochemistry and DNA sequences). The aim of this study was to assess the relationships and boundaries among Eastern Pacific Aplysina species using morphological, biochemical and molecular data. For this, a collection of sponges of the genus Aplysina from the Mexican Pacific was studied on the basis of their morphological, chemical (chitin composition), and molecular markers (mitochondrial COI and nuclear ribosomal rDNA: ITS1-5.8-ITS2). Three morphological species were identified, two of which are new to science. A. clathrata sp. nov. is a yellow to yellow-reddish or -brownish sponge, characterized by external clathrate-like morphology; A. revillagigedi sp. nov. is a lemon yellow to green, cushion-shaped sometimes lobate sponge, characterized by conspicuous oscules, which are slightly elevated and usually linearly distributed on rims; and A. gerardogreeni a known species distributed along the Mexican Pacific coast. Chitin was identified as the main structural component within skeletons of the three species using FTIR, confirming that it is shared among Verongida sponges. Morphological differences were confirmed by DNA sequences from nuclear ITS1-5.8-ITS2. Mitochondrial COI sequences showed extremely low but diagnostic variability for Aplysina revillagigedi sp. nov., thus our results corroborate that COI has limited power for DNA-barcoding of sponges and should be complemented with other markers (e.g. rDNA). Phylogenetic analyses of Aplysina sequences from the Eastern Pacific and Caribbean, resolved two allopatric and reciprocally monophyletic groups for each region. Eastern Pacific species were grouped in general accordance with the taxonomic hypothesis based on morphological characters. An identification key of Eastern Pacific Aplysina species is presented. Our results constitute one of the first approximations to integrative taxonomy, phylogeny and evolutionary biogeography of Eastern Pacific marine sponges; an approach that will significantly contribute to our better understanding of their diversity and evolutionary history. © 2012 Cruz et al.
chitin, genomic DNA, mitochondrial DNA, molecular marker, ribosome DNA, allopatry, Aplysina clatharata, Aplysina gerardogreeni, Aplysina revillagigedi, article, DNA barcoding, DNA sequence, genetic polymorphism, genetic variability, infrared spectros CIENCIAS FÍSICO MATEMÁTICAS Y CIENCIAS DE LA TIERRA CIENCIAS DE LA TIERRA Y DEL ESPACIO OCEANOGRAFÍA OCEANOGRAFÍA
MAHENDAR THUDI Abhishek Bohra Spurthi Nayak Trushar Shah R. Varma Penmetsa Nepolean Thirunavukkarasu Pooran Gaur Pawan Kulwal Hari Upadhyaya Polavarapu Kavi Kishor Rajeev Varshney (2011, [Artículo])
Molecular Markers Recombinant Inbred Lines CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA GENETIC MARKERS MICROSATELLITES PLANTS DNA CHICKPEAS ARRAYS TECHNOLOGY CHROMOSOME MAPPING GENETIC VARIATION GENOTYPES MOLECULAR CLONING
Comprehending the evolution of gene editing platforms for crop trait improvement
deepmala sehgal Apekshita Singh SoomNath Raina (2022, [Artículo])
Cas9 Base Editing Prime Editing Epigenome Editing CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA CRISPR ABIOTIC STRESS ARABIDOPSIS CROP IMPROVEMENT DNA ELECTROPORATION GENE EDITING RICE WHEAT
Fuai Sun XUECAI ZHANG Haoqiang Yu (2022, [Artículo])
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Philomin Juliana (2023, [Artículo])
CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA DNA MARKER-ASSISTED SELECTION QUANTITATIVE TRAIT LOCI CROP IMPROVEMENT
Abdelfattah DABABAT Yong Suk Chung (2023, [Artículo])
Biological Indexing Sequencing Analysis Citrus Orchards CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA PHYLOGENETIC ANALYSIS CITRUS RNA DNA
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Alexey Morgounov Cecile Ben Susanne Dreisigacker Laurent Gentzbittel awais rasheed Timur Savin Sergey Shepelev Vladimir Shamanin (2024, [Artículo])
DNA Markers Grain Yield CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA CEREALS DNA GENOTYPE ENVIRONMENT INTERACTION GRAIN YIELDS SPRING WHEAT
Alison Bentley Charles Chen Nunzio D'Agostino (2022, [Artículo])
Allele Mining High-Throughput Phenotyping Genomic Estimated Breeding Value CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA CROP IMPROVEMENT DNA CHROMOSOME MAPPING GENETIC LINKAGE GENOMES GENOTYPING GERMINATION HEAT STRESS QUALITY CONTROL SINGLE NUCLEOTIDE POLYMORPHISM TRITICUM AESTIVUM GENETIC DIVERSITY (AS RESOURCE) HIGH-THROUGHPUT SEQUENCING
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