Evaluación de la primera madurez sexual del huachinango del Pacífico (Lutjanus peru) nacido en cautiverio

First sexual maturity evaluation of the Pacific red snapper (Lutjanus peru) born in captivity

MILTON ALEJANDRO SPANOPOULOS ZARCO José Antonio Estrada Godínez JUAN CARLOS PEREZ URBIOLA VICENTE GRACIA LOPEZ Felipe de Jesús Ascencio Valle Manuel Carrillo Marcos Fabian Quiñones Arreola MINERVA CONCEPCION MALDONADO GARCIA (2016, [Artículo])

"El huachinango del Pacífico, Lutjanus peru, es una especie atractiva para la acuicultura. Sin embargo, su reproducción en cautiverio en condiciones controladas no había sido factible, debido a la falta de conocimiento sobre las condiciones de manejo y el proceso reproductivo. En este estudio se evaluó la primera madurez sexual en cautiverio del huachinango del Pacífico, nacidos en el 2009 en el CIBNOR. La primera madurez sexual se determinó a los cuatro años de edad, con un peso promedio de 2.829 ± 80,9 g y longitud promedio de 540,3 ± 4,6 mm. Los primeros desoves ocurrieron en junio 2013, con la sincronización reproductiva entre machos y hembras en cautiverio, que coincidió cuando el fotoperiodo fue de 13 h luz y 11 h de obscuridad, con un aumento de temperatura del agua del estanque de 24,81 ± 1,4°C. El desove terminó en diciembre, con una disminución de temperatura 21,2 ± 1,5°C y 10 h luz. Se determinó la proporción sexual de 1:3,2 (hembra: macho). El tamaño de los huevos y la gota lipídica, disminuyeron conforme avanzó la temporada reproductiva..Se evaluó el nivel hormonal (estradiol, testosterona y 11-keto-testosterona) durante tres años. En cautiverio se determinó un ciclo reproductivo definido, en dos periodos; un periodo de reposo de diciembre a mayo, y un periodo reproductivo de junio a noviembre."

"The Pacific red snapper Lutjanus peru, is an attractive species for aquaculture. Its reproduction in captivity under controlled conditions has not been feasible, due to the lack of knowledge on its basic reproductive biology and management conditions. For the first time, we evaluated the first sexual maturity stage of captive Pacific red snapper, born in 2009 at CIBNOR. The first sexual maturity was reached in four years with an average weight of 2,829 ± 80.9 g and average size of 540.3 ± 4.6 mm. Spawning occurred in June 2013, with a reproductive synchronization between males and females, which coincides with a photoperiod of 13 h of light and 11 h of darkness, an increase in the water ponds temperature to 24.81 ± 1.4°C. The final spawning occurred in December with a decrement of the water ponds temperature to 21.2 ± 1.5°C and 10 h light. The sex ratio was determined as 1:3.2 (female: male). The size of eggs and lipid drop decreased as the spawning season progressed. Hormone levels (estradiol, testosterone and 11-keto-testosterone), was evaluated for three years. We determined a defined reproductive cycle for the species in captivity in two periods: 1) a rest period, from December to May, and 2) a reproductive period from June to November."

Lutjanus peru, primera madurez, desoves naturales, acuicultura. Lutjanus peru, first maturity, natural spawning, aquaculture. CIENCIAS FÍSICO MATEMÁTICAS Y CIENCIAS DE LA TIERRA CIENCIAS DE LA TIERRA Y DEL ESPACIO OCEANOGRAFÍA OCEANOGRAFÍA ACUICULTURA MARINA OCEANOGRAFÍA ACUICULTURA MARINA

Gene expression in primary hemocyte culture of the Pacific white shrimp Penaeus vannamei infected with different white spot syndrome virus (WSSV) strains

Delia Patricia Parrilla Taylor REGINA ELIZONDO GONZALEZ Jesús Neftalí Gutiérrez Rivera SILVIA ALEJANDRA GARCIA GASCA NORBERTO VIBANCO PEREZ MA. DE JESUS DURAN AVELAR RICARDO VAZQUEZ JUAREZ (2022, [Artículo])

"Five previously analyzed white spot syndrome virus (WSSV) strains from northwest Mexico,differing in their genome architecture as well as in virulence, were selected (high virulence JP and LG strains;moderate virulence GVE and DIV strains; and low virulence LC10 strain) to evaluate pathogenesis response in vitro. Expression of phagocytosis-activating protein PAP, manganese superoxide dismutase MnSOD and peroxiredoxin PRX, and two genes of immediate-early expression (IE1 and WSSV304) were measured by qPCR in a primary hemocyte cell culture from Penaeus vannamei at 1, 3, 6, 12, and 24 h post-infection (hpi). PAP expression was significantly higher at 1 and 3 hpi, and JP and LC10 strains induced the highest expression. The response of MnSOD was high at 1 hpi, and a significant increase in PRX expression was detected at 3 hpi, probably due to the occurrence of an oxidative burst; expression levels of MnSOD and PRX were significantly higher at 1 and 3 hpi, respectively, induced by the LG strain (high virulence), suggesting an acute response. In general, expression of most immune-related - genes decreased after the initial hours of infection. Expression levels of IE1 and WSSV304 were exceptionally high at 1 hpi in almost all five WSSV analyzed strains,confirming their efficient mechanism for replication and viral fitness. The results of this study do not show an accurate link between the genome size and WSSV virulence of the strains, albeit the strain with the smallest genome showed the highest virulence. All strains induced an early immune response in heterogeneous ways."

Penaeus vannamei, virulence, gene expression, viral fitness, viral pathogenesis, immune response BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA BIOLOGÍA ANIMAL (ZOOLOGÍA) PATOLOGÍA ANIMAL PATOLOGÍA ANIMAL

Editorial: Evolution of abiotic stress responses in land plants

Ana Luisa Garcia-Oliveira (2023, [Artículo])

Land Plants Molecular Responses Plant Stress Biology CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA ABIOTIC STRESS TERRESTRIAL PLANTS CLIMATE CHANGE

Mapping of SARS-CoV-2 spike protein evolution during the first and second waves of COVID-19 infections in India

Shailendra Sharma Avinash Kumar Apekshita Singh deepmala sehgal Shailendra Goel SoomNath Raina (2022, [Artículo])

Immune Evasion Mutation Analysis Novel Spike Mutations Reduced Neutralization SARS-CoV-2 CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA COVID-19 NEUTRALIZATION SPIKES

Integrative transcriptome and metabolome analysis reveals the mechanisms of light-induced pigmentation in purple waxy maize

XUECAI ZHANG Yunbi Xu Prasanna Boddupalli (2023, [Artículo])

Pigment Accumulation Light Response CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA ANTHOCYANINS LIGHT METABOLOMICS TRANSCRIPTOMICS WAXY MAIZE

De Novo assembly and annotation of the Pacific calico scallop (Argopecten ventricosus) transcriptome for immune-related gene discovery

RUTH ESCAMILLA MONTES GENARO DIARTE PLATA GABRIELA BERENICE MENDOZA MALDONADO Aarón Barraza Celis Carlos Eliud Angulo Valadez Seyed Hossein Hoseinifar Jesús Arturo Fierro Coronado ANTONIO LUNA GONZALEZ (2022, [Artículo])

"Invertebrates' immune defense mechanisms play a critical role in pathogen recognition and elimination. De novo assembly and annotation of the Argopecten ventricosus transcriptome were performed for the immune-related gene identification. Scallops (height: 4.4 cm) were challenged with inactivated Vibrio parahaemolyticus IPNGS16. The RNA from different tissues was pooled for a single cDNA library construction sequenced by NextSeq 500 platform 2×75 paired‐end chemistry. Before de novo assembling with Trinity, reads were analyzed with FastQC, Trimmomatic, and Prinseq. Assembled sequences were analyzed by CD-HIT-EST and TransDecoder. The corresponding annotation was performed against NCBI-nr, RefSeq protein, and KAAS (KEGG) databases. The Trinity assembly yielded 107,516 contigs. TransDecoder yielded 25,285 sequences as CDSs of which, 16,123 were annotated against the NCBI-nr protein, most of them scored with Crassostrea gigas data. Gene ontology mapped sequences (15,262) were classified in molecular functions (~13,000), cellular components (~11,000), and biological processes (~13,000). The KAAS analysis showed biological categories for metabolism (13%), cellular processes (12%), genetic information processing (10%), organismal systems (19%), environmental information (13%), and human diseases (33 %). Within the organismal systems, 467 immune-related genes (KO) were identified. Sixty-four immune-related genes were annotated/blasted against the NCBI-nr and RefSeq protein databases. An RT-qPCR was performed to analyze the expression level of immune-related genes obtained in the transcriptome analysis in scallops (height 4.5 cm) treated with probiotic bacilli added to culture water. Bacilli significantly increased the expression of the HSP70 and PGRP genes. The gene transcripts analysis of A. ventricosus will better understand its immune response against pathogens in culture systems."

Argopecten ventricosus, calico scallop, transcriptome, bivalve, immune genes BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA BIOLOGÍA ANIMAL (ZOOLOGÍA) INMUNOLOGÍA INMUNOLOGÍA

Propuesta de mejora para la integración del equipo de salud en el código azul en áreas críticas adulto

DANIEL GARCIA NAVARRETE (2023, [Otro, Trabajo terminal, especialidad])

La presente investigación se realizará en un hospital de segundo nivel de atención de la ciudad de Cuernavaca, el código azul es un sistema de alarma que existe en la mayoría de los hospitales, está conformado por un equipo multidisciplinario de salud que se encuentra capacitado y es convocado de manera inmediata con funciones previamente asignadas para poder intervenir de forma oportuna, eficaz y con calidad a pacientes en paro cardiopulmonar. Este trabajo de investigación propone la integración del equipo de salud en código azul.

Se menciona como fue la historia de la formación de los grupos de reanimación cardiopulmonar y la organización del código azul; fue el primer modelo de estos sistemas y ha demostrado un impacto positivo en la morbimortalidad de los pacientes, la conexión de la teórica Patricia Benner de principiante a experto, se describe como se vive un código azul en el área hospitalaria, la necesidad de estructurar tarjetas que identifique que rol tiene cada uno de los integrantes del código azul, el personal de salud tiene que tener capacidad de reconocer la emergencia del evento, realizando oportunamente las intervenciones del código azul. Metodología adecuada para esta investigación: se decidió un estudio de tipo cuantitativo, longitudinal, observacional, cuasiexperimental y de intervención educativa, será dirigido a todo profesional de salud; área médica, enfermería, camilleros, residentes, pasantes y estudiantes de ambas profesiones de los diferentes turnos que laboran en las áreas críticas del hospital. Se pretende aplicar dos instrumentos de evaluación diagnóstica y de habilidades previo a la capacitación curso- taller con la finalidad de fortalecer los conocimientos y habilidades y posteriormente aplicar los mismos instrumentos de evaluación final. Se implementará un pizarrón en cada servicio, donde describa el rol de la actividad que tendrá cada integrante que conforme el equipo de código azul, así mismo se proporcionará el manual de procedimientos de código azul, guía de práctica clínica impresa que se ubicaran en un lugar estratégico para su consulta y gafetes que describa la función que le corresponde a cada uno de los miembros.

This research will be carried out in a second level care hospital in the city of Cuernavaca. The code blue is an alarm system that exists in most hospitals; it is made up of a multidisciplinary health team that is trained and immediately summoned with previously assigned functions in order to intervene in a timely, effective and quality manner in patients in cardiopulmonary arrest. This research work proposes the integration of the health team in code blue.

It mentions the history of the formation of cardiopulmonary resuscitation groups and the organization of the code blue; it was the first model of these systems and has shown a positive impact on patient morbidity and mortality, the connection of the theorist Patricia Benner from beginner to expert, it describes how a code blue is experienced in the hospital area, the need to structure cards that identify the role of each of the members of the code blue, the health personnel must have the ability to recognize the emergency event, performing timely interventions of the code blue. Appropriate methodology for this research: a quantitative, longitudinal, observational, quasi-experimental, quasi-experimental and educational intervention study was decided, and it will be directed to all health professionals; medical area, nursing, orderlies, residents, interns and students of both professions of the different shifts that work in the critical areas of the hospital. It is intended to apply two diagnostic and skills evaluation instruments prior to the training course-workshop in order to strengthen knowledge and skills and then apply the same instruments for the final evaluation. A blackboard will be implemented in each service, describing the role of the activity that each member of the code blue team will have, as well as the code blue procedure manual, printed clinical practice guide that will be placed in a strategic place for consultation and badges that describe the function that corresponds to each of the members.

MEDICINA Y CIENCIAS DE LA SALUD CIENCIAS MÉDICAS código azul, integración, equipos de respuesta rápida, personal de salud, paro cardiopulmonar. code blue, integration, rapid response teams, health personnel, cardiopulmonary arrest.