Repositorio Nacional

"WRKY transcription factors (TFs) play key roles in plant defense responses through phytohormone signaling pathways. However, their functions in tropical fruit crops, especially in banana, remain largely unknown. Several WRKY genes from the model plants rice (OsWRKY45) and Arabidopsis (AtWRKY18, AtWRKY60, AtWRKY70) have shown to be attractive TFs for engineering disease resistance. In this study, we isolated four banana cDNAs (MaWRKY18, MaWRKY45, MaWRKY60, and MaWRKY70) with homology to these rice and Arabidopsis WRKY genes. The MaWRKY cDNAs were isolated from the wild banana Musa acuminata ssp. malaccensis, which is resistant to several diseases of this crop and is a progenitor of most banana cultivars. The deduced amino acid sequences of the four MaWRKY cDNAs revealed the presence of the conserved WRKY domain of ~60 amino acids and a zinc-finger motif at the N-terminus. Based on the number of WRKY repeats and the structure of the zinc-finger motif, MaWRKY18 and MaWRKY60 belong to group II of WRKY TFs, while MaWRKY45 and MaWRKY70 are members of group III. Their corresponding proteins were located in the nuclei of onion epidermal cells and were shown to be functional TFs in yeast cells. Moreover, expression analyses revealed that the majority of these MaWRKY genes were upregulated by salicylic acid (SA) or methyl jasmonate (MeJA) phytohormones, although the expression levels were relatively higher with MeJA treatment. The fact that most of these banana WRKY genes were upregulated by SA or MeJA, which are involved in systemic acquired resistance (SAR) or induced systemic resistance (ISR), respectively, make them interesting candidates for bioengineering broad-spectrum resistance in this crop."

Banana Transcription factor WRKY Defense phytohormones Salicylic acid Methyl jasmonate SAR ISR Broad-spectrum resistance BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA GENÉTICA GENÉTICA

Modular ontology to support manufacturing SMEs toward industry 4.0

ZAIDA ANTONIETA MORA ALVAREZ OSCAR HERNANDEZ URIBE RAMON ALBERTO LUQUE MORALES LEONOR ADRIANA CARDENAS ROBLEDO (2023, [Artículo])

Industry 4.0 (I4.0) implementation is a hot topic among manufacturing organizations to reach smart factory status and integrate a fully connected ecosystem. Achieving such a transition presents notable challenges for Small and Medium Enterprises (SMEs) since they often face resource and skilled personnel limitations. This study developed a domain ontology to represent various stages of maturity toward I4.0 implementation. Ontology provides a tool for SMEs to self-assess in situations of machines, processes, and factories for the dimensions of control, integration, and intelligence. This study focused on the identification of classes and relationships according to I4.0 implementation situations in the context of a manufacturing setting, the reuse of ontologies related to the domain of observations to model situations, and the creation and validation of the ontology through the information obtained from the questionnaires applied to SMEs. Finally, the ontology delivers a tool to understand SMEs' current state concerning I4.0 implementation and plan based on informed decisions about the maturity state and the technology required to advance to the next stage in their manufacturing processes.

This study was partially supported by the grants CONAHCYT-CIATEQ CVU 899567 and 162867 and CONAHCYT SNI.

We express our gratitude to Teresa Novales Hernandez for the library support.

Domain ontology Industry 4.0 SMEs Smart factory SPARQL Semantic web INGENIERÍA Y TECNOLOGÍA CIENCIAS TECNOLÓGICAS OTRAS ESPECIALIDADES TECNOLÓGICAS OTRAS OTRAS

Unpacking the intra-household decision-making process among wheat growers in Bihar, India

Hom Nath Gartaula (2022, [Objeto de congreso])

CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA WHEAT CROP MANAGEMENT SMALLHOLDERS GENDER HOUSEHOLDS

Global surveillance summary

David Hodson (2022, [Objeto de congreso])

CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA WHEAT RUSTS SURVEILLANCE SYSTEMS

Metrics for optimum allocation of resources on the composition and characterization of crop collections: The CIMMYT wheat collection as a proof of concept

M. Humberto Reyes-Valdés Juan Burgueño Carolina Sansaloni Thomas Payne Rosa Angela Pacheco Gil (2022, [Artículo])

Crop Genebanks Optimization Relative Balance CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA CROPS GENE BANKS WHEAT

Balancing quality with quantity: a case study of UK bread wheat

Nick Fradgley Keith Gardner Stéphanie M. Swarbreck Alison Bentley (2023, [Artículo])

Grain Protein Content Environmental Sustainability End-Use Quality Modern Bread Baking Methods CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA GRAIN PROTEIN CONTENT HISTORY QUALITY WHEAT YIELDS