Intercalibración de las pruebas con Daphnia magna y Pseudokirchneriella subcapitata en México: herramientas potenciales para el monitoreo ambiental

ANIA MENDOZA CANTU PATRICIA RAMIREZ ROMERO YOLANDA PICA GRANADOS ALMA SOCORRO SOBRINO FIGUEROA (2013, [Artículo])

En México, como en otros países en desarrollo, el conocimiento sobre el grado de contaminación y sus efectos en los ecosistemas locales se ha generado siguiendo objetivos de investigación específicos que emplean metodologías de distinta naturaleza. Dado que aún existen vacíos de información en el tema, es importante impulsar el uso generalizado de pruebas ecotoxicológicas que empleen protocolos estandarizados y que sirvan como herramientas regulatorias para establecer medidas integrales de protección al ambiente. Por lo anterior, el presente estudio tuvo como objetivo demostrar, a través de ejercicios de intercalibración, previo desarrollo de talleres de homologación de capacidades, el potencial de los protocolos de ensayo con Daphina magna y Pseudokirchneriella subcapitata (antes Selenastrum capricornutum) para generar resultados confiables en laboratorios con poca experiencia en este campo. Esto permitirá impulsar el empleo de herramientas bioanalíticas en México para el monitoreo ambiental, las cuales favorecen la evaluación integral de los problemas de contaminación en los cuerpos de agua. Los resultados de los ejercicios de calibración interna de los laboratorios demostraron que en un corto plazo es posible lograr una repetibilidad aceptable en las pruebas y un desempeño adecuado de los protocolos, lo cual apoya la idea de que estos protocolos son pertinentes para su empleo en programas de monitoreo ambiental, aún en países donde actualmente se cuenta con pocos recursos humanos capacitados y laboratorios aptos para tal fin.

Intercalibración Daphnia magna Monitoreo del ambiente Ambiente acuático BIOLOGÍA Y QUÍMICA

MEDICIÓN DE TEMPERATURA EN OBJETOS DE FASE MEDIANTE UN INTERFERÓMETRO DE MACH-ZEHNDER Y UN INTEFERÓMETRO DE WOLLASTON

Joao Francisco Trujillo Martinez (2022, [Tesis de maestría])

"Se implementarán de manera independiente, las técnicas de interferometría de Mach-Zehnder y de interferometría de Wollaston. Lo anterior para obtener información importante del comportamiento termodinámico de un objeto de fase axisimétrico. Debido a la naturaleza interferómetrica de ambos métodos, serán necesarios dos haces que interfirieran. Por lo anterior, ambas técnicas se mostrarán sensibles al ruido externo, sin embargo, al requerir la interferometría de Wollaston una sola trayectoria para los dos haces que interfieren, se espera que sea esta técnica, una técnica más robusta que la técnica interferométrica de Mach-Zehnder, la cual la hará más propicia para su implementación en un entorno industrial."

Temperatura Metrología Diseño óptico Interferómetro INGENIERÍA Y TECNOLOGÍA CIENCIAS TECNOLÓGICAS TECNOLOGÍA INDUSTRIAL EQUIPO INDUSTRIAL EQUIPO INDUSTRIAL

Agricultural emissions reduction potential by improving technical efficiency in crop production

Arun Khatri-Chhetri Tek Sapkota sofina maharjan Paresh Shirsath (2023, [Artículo])

CONTEXT: Global and national agricultural development policies normally tend to focus more on enhancing farm productivity through technological changes than on better use of existing technologies. The role of improving technical efficiency in greenhouse gas (GHG) emissions reduction from crop production is the least explored area in the agricultural sector. But improving technical efficiency is necessary in the context of the limited availability of existing natural resources (particularly land and water) and the need for GHG emission reduction from the agriculture sector. Technical efficiency gains in the production process are linked with the amount of input used nd the cost of production that determines both economic and environmental gains from the better use of existing technologies. OBJECTIVE: To assess a relationship between technical efficiency and GHG emissions and test the hypothesis that improving technical efficiency reduces GHG emissions from crop production. METHODS: This study used input-output data collected from 10,689 rice farms and 5220 wheat farms across India to estimate technical efficiency, global warming potential, and emission intensity (GHG emissions per unit of crop production) under the existing crop production practices. The GHG emissions from rice and wheat production were estimated using the CCAFS Mitigation Options Tool (CCAFS-MOT) and the technical efficiency of production was estimated through a stochastic production frontier analysis. RESULTS AND CONCLUSIONS: Results suggest that improving technical efficiency in crop production can reduce emission intensity but not necessarily total emissions. Moreover, our analysis does not support smallholders tend to be technically less efficient and the emissions per unit of food produced by smallholders can be relatively high. Alarge proportion of smallholders have high technical efficiency, less total GHG emissions, and low emissions intensity. This study indicates the levels of technical efficiency and GHG emission are largely influenced by farming typology, i.e. choice and use of existing technologies and management practices in crop cultivation. SIGNIFICANCE: This study will help to promote existing improved technologies targeting GHG emissions reduction from the agriculture production systems.

Technical Efficiency Interventions CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA MITIGATION PRODUCTIVITY CROP PRODUCTION GREENHOUSE GAS EMISSIONS

Más allá de los colores del amaranto

JESUS ALFREDO ARAUJO LEON Víctor Aguilar Hernández Ivonne Sánchez del Pino SERGIO RUBEN PERAZA SANCHEZ ROLFFY RUBEN ORTIZ ANDRADE Ligia Guadalupe Brito Argáez (2022, [Artículo])

El amaranto tiene una amplia gama de colores atrayentes, desde amarillos, violetas hasta rojos intensos. Esta heterogeneidad de tonalidades llamó la atención de civilizaciones que se desarrollaron en todas las latitudes del continente americano, como es el caso del imperio azteca que integró este cultivo a su cosmovisión ceremonial para ofrendas a diferentes deidades, dejando una herencia prehispánica cautivadora de su historia. Más allá de los colores, el presente trabajo tiene como objetivo abordar las propiedades nutricionales y beneficios para la salud humana de la diversidad de fitoquímicos que son responsables de los interesantes colores del amaranto.

AMARANTHACEAE AMARANTHUS BETALAINAS METABOLITOS SECUNDARIOS MEXICO SALUD HUMANA BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA BIOLOGÍA VEGETAL (BOTÁNICA) DESARROLLO VEGETAL DESARROLLO VEGETAL

Sistema ICAM-Riego para Internet

JOSE JAVIER RAMIREZ LUNA Olga Xochitl Cisneros Estrada (2012, [Documento de trabajo])

El objetivo del proyecto ha sido desarrollar el Sistema ICAM-Riego Internet. Dicho sistema pretende cubrir la mayoría de las necesidades en cuanto a accesibilidad y procesamiento de información tanto en distritos como en módulos de riego de México. De igual manera, el proyecto muestra el trabajo realizado en la actualización de los diversos sistemas que conforman el ICAM-Riego emigrando este a la modalidad ICAM-Riego en Internet. Así mismo se han desarrollado sistemas que se complementan con novedosos instrumentos de medición, los cuales permiten obtener información en tiempo real de diversas variables que afectan el entorno agrícola.

Informes de proyectos Procesamiento automatizado de datos Programas de computación Internet Distritos de riego INGENIERÍA Y TECNOLOGÍA

LGBT+ media on the Internet: experiences of communication and information in Mexico

RAUL ANTHONY OLMEDO NERI (2023, [Artículo, Artículo])

Two digital LGBT+ media in Mexico are analyzed to understand the implications of their operation and their articulation with the informational and social needs of sex-gender populations. It starts from the communicational perspective to conceptualize these projects as exercises in technological appropriation but highlighting their empirical intersections with other analytical frameworks. Through the method of systematizing the experience, the trajectories of ANODIS and Seis Franjas Mx have been recovered; through semi-structured interviews conducted with their co-founders in February 2022, the reasons, horizons and challenges that these projects have faced are analyzed. The results show that the lack of LGBT+ content and representations in the media motivate the creation of these projects, which articulate a sense of collaboration and sociality with other users belonging to sex-gender communities. In addition, the people participating in these projects are part of the LGBT+ populations, becoming them producers and consumers of information that claim the gender-identity dimension in the content. Likewise, these initiatives are specified in the youth stage of their co-founders, which refers to rethinking the role of LGBT+ youth in the new forms of activism and socialization mediated by Internet. Finally, given the progressive formation of this area of ​​knowledge and the lack of consensus on its definition, it is proposed to name this interdisciplinary field of study as LGBT+ Communicational Studies, to show an epistemological perspective from communication and a Latin American ontological position.

Medios de comunicación Internet LGBT Jóvenes redes sociales de apoyo CIENCIAS SOCIALES CIENCIAS SOCIALES Media LGBT youth

A Flexible, Extensible, Machine-Readable, Human-Intelligible, and Ontology-Agnostic Metadata Schema (OIMS)

Gideon Kruseman (2022, [Artículo])

Reusability Java Script Object Notation CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA DATA MANAGEMENT INTEROPERABILITY METADATA SOCIOECONOMIC ASPECTS

Evaluación de la acumulación mitocondrial de estrógenos sintéticos mito-dirigidos en un modelo in vitro

Evaluation of mitochondrial accumulation of mito-targeted synthetic estrogens on in vitro model

Magdiel Orozco Valdivia (2023, [Tesis de maestría])

La mitocondria es un organelo que cumple funciones esenciales en el mantenimiento de las células eucariotas y su disfunción está asociada a la progresión de enfermedades neurodegenerativas, metabólicas y cardiovasculares. Por lo anterior es necesario encontrar tratamientos que mejoren la función mitocondrial. Los estrógenos son hormonas que tienen diversos efectos benéficos sobre las células. Entre estos efectos, se ha observado que las hormonas inciden en la función mitocondrial, por lo que son un potencial tratamiento para las enfermedades asociadas a la disfunción mitocondrial. Una alternativa es mediante los compuestos mito-dirigidos, moléculas con actividad biológica que son acoplados a cationes lipofílicos con la capacidad de acumularse a mayor concentración en la mitocondria respecto a las sustancias no mito-dirigidas. Por lo tanto, se plantea conferir la propiedad acumulativa a los estrógenos mediante el acoplamiento de trifenilfosfonio, un catión lipofílico recurrentemente usado para la generación de mito-dirigidos. Para el presente trabajo se sintetizaron dos estrógenos mito-dirigidos. Este proyecto evalúa la acumulación mitocondrial de los estrógenos mito-dirigidos después de una exposición de estos en un modelo in vitro sensible a estrógenos, comparando su acumulación con la del estrógeno no mito-dirigido. Los resultados indican que los estrógenos mito-dirigidos se acumulan más en las mitocondrias que el estrógeno no mito-dirigido y demuestra que los estrógenos mito-dirigidos tienen capacidad acumulativa, esto permite sentar las bases de un tratamiento hormonal dirigido a las mitocondrias que se pueda aplicar a enfermedades asociadas a la disfunción mitocondrial.

Mitochondria are essential organelles of eukaryotic cells, and their dysfunction is associated with the onset and development of neurodegenerative, metabolic, and cardiovascular diseases. Thus, it is important to develop treatments to improve mitochondrial function. One of the options are estrogens, these hormones have a protective role in cellular function by participating in the regulation of mitochondrial function, making estrogens candidates to be a potential treatment for diseases related to mitochondrial dysfunction. One of the alternative treatments are mito-targeted molecules, which are biologically active molecules conjugated with lipophilic cations. These mito-targeted molecules can accumulate inside mitochondria more than non-targeted molecules. This project aims to synthesize mito-targeted estrogens by conjugating a lipophilic cation, triphenylphosphonium, to a parent estrogenic compound. Our hypothesis is that these novel molecules will accumulate in the interior of the mitochondria and will have more biological activity than the non-targeted estrogen. We synthesized two mito-targeted estrogens for this project and evaluated the mitochondrial accumulation in an estrogen-sensitive in vitro model, comparing their accumulation to non-targeted estrogens. The results indicate mito-targeted estrogens accumulate in mitochondria more than the non-targeted estrogens, showing that mito-targeted estrogens have an accumulation capacity. This insight will make new treatments for diseases related to mitochondrial dysfunction.

estrógenos, mito-dirigido, disfunción mitocondrial, acumulación, mitocondria estrogens, mitochondria, mito-targeted, accumulation, mitochondrial dysfunction BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA BIOLOGÍA HUMANA HORMONAS HORMONAS

Morphological Alterations in Human Skeletal Muscle Cells Exposed to di-(2-Ethylhexyl) Phthalate (dehp) in Primary Cell Culture Conditions

Alteraciones Morfológicas en Células Musculares Esqueléticas Humanas Expuestas a Ftalato de di-(2-etilhexilo) (dehp) en Condiciones de Cultivo Celular Primario

Pablo Hernández Almaraz ORLANDO LUGO LUGO Ramón Gaxiola Robles OSCAR KURT BITZER QUINTERO Luis Javier Ramírez Jirano Elizabeth Brassea Pérez TANIA ZENTENO SAVIN (2022, [Artículo])

"Di-(2-ethylhexyl) phthalate (DEHP) is among the most common plasticizer additives that humans are in contact with daily. DEHP can be released from plastic and enter the human body, whereby it is metabolized and transformed into oxidative hydrophilic molecules. Clinical follow-ups in patients exposed to this phthalate and investigations in cultures of several cell types have provided information on its effects. For example, it is associated with inhibition of diploid human cell development and morphological changes in cultured germ cells. Although skeletal muscle represents around 50 % of the human body mass, knowledge about the effects of DEHP on this tissue is poor. Cultured skeletal muscle cells were exposed to DEHP (1 mM) for 13 days with the aim of exploring and evaluating some of the potential morphological effects. Three culture development parameters and nine cell characteristics were monitored during the bioassay. At 13 days, growth area, cell viability, and concentration of total proteins were lower in DEHP exposed than in control cells. Cell width and area, as well as the diameter of the nucleus and nucleolus, were greater in exposed cells than in control cells. These are interpreted as signs of cytotoxicity and suggest potential adverse effects on the development of skeletal muscle cells from DEHP exposure, as reported for other cell types."

Emerging pollutants, Morphometry, Phthalates, Plasticizers, Toxicity MEDICINA Y CIENCIAS DE LA SALUD CIENCIAS MÉDICAS BIOLOGÍA HUMANA FISIOLOGÍA DEL MÚSCULO FISIOLOGÍA DEL MÚSCULO

Sustainability evaluation of contrasting milpa systems in the Yucatán Peninsula, Mexico

Santiago Lopez-Ridaura Tania Carolina Camacho Villa (2023, [Artículo])

The milpa agroecosystem is an intercropping of maize, beans, squash and other crops, developed in Mesoamerica, and its adoption is widely variable across climates and regions. An example of particular interest is the Yucatan Peninsula in Mexico, which holds highly diverse milpas, drawing on ancestral Mayan knowledge. Traditional milpas have been described as sustainable resource management models, based on long rotations within a slash-and-burn cycle in forest areas. Nevertheless, due to modernization and intensification processes, new variants of the approach have appeared. The objective of this study was to evaluate the sustainability of three milpa systems (traditional, continuous, and mechanized) in four case studies across the Peninsula, with emphasis on food self-sufficiency, social inclusion and adoption of innovations promoted by a development project. The Framework for the Evaluation of Agroecosystems using Indicators (MESMIS, for its Spanish acronym) was used for its flexible, participatory approach. A common group of indicators was developed despite regional differences between study cases, with a high level of farmer participation throughout the iterative process. The results show lower crop yields in traditional systems, but with lower inputs costs and pesticide use. In contrast, continuous milpas had higher value in terms of crop diversity, food security, social inclusion, and innovation adoption. Mechanized milpas had lower weed control costs. Profitability of cash crops and the proportion of forest were high in all systems. Highly adopted innovations across milpa types and study cases included spatial crop arrangement and the use of residues as mulches. However, most innovations are not adapted to local conditions, and do not address climate change. Further, women and youth participation is low, especially in traditional systems.

Milpa Intensification Processes Women and Youth Participation CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA SUSTAINABILITY INTERCROPPING FOOD SECURITY INNOVATION SOCIAL INCLUSION AGROECOSYSTEMS CASE STUDIES