Genome-wide association analyses of agronomic traits and Striga hermonthica resistance in pearl millet

Hussein Shimelis Chris Ojiewo Abhishek Rathore (2023, [Artículo])

Pearl millet (Pennisetum glaucum [L.] R. Br.) is a nutrient-dense, relatively drought-tolerant cereal crop cultivated in dry regions worldwide. The crop is under-researched, and its grain yield is low (< 0.8 tons ha−1) and stagnant in the major production regions, including Burkina Faso. The low productivity of pearl millet is mainly attributable to a lack of improved varieties, Striga hermonthica [Sh] infestation, downy mildew infection, and recurrent heat and drought stress. Developing high-yielding and Striga-resistant pearl millet varieties that satisfy the farmers’ and market needs requires the identification of yield-promoting genes linked to economic traits to facilitate marker-assisted selection and gene pyramiding. The objective of this study was to undertake genome-wide association analyses of agronomic traits and Sh resistance among 150 pearl millet genotypes to identify genetic markers for marker-assisted breeding and trait introgression. The pearl millet genotypes were phenotyped in Sh hotspot fields and screen house conditions. Twenty-nine million single nucleotide polymorphisms (SNPs) initially generated from 345 pearl millet genotypes were filtered, and 256 K SNPs were selected and used in the present study. Phenotypic data were collected on days to flowering, plant height, number of tillers, panicle length, panicle weight, thousand-grain weight, grain weight, number of emerged Striga and area under the Striga number progress curve (ASNPC). Agronomic and Sh parameters were subjected to combined analysis of variance, while genome-wide association analysis was performed on phenotypic and SNPs data. Significant differences (P < 0.001) were detected among the assessed pearl millet genotypes for Sh parameters and agronomic traits. Further, there were significant genotype by Sh interaction for the number of Sh and ASNPC. Twenty-eight SNPs were significantly associated with a low number of emerged Sh located on chromosomes 1, 2, 3, 4, 6, and 7. Four SNPs were associated with days-to-50%-flowering on chromosomes 3, 5, 6, and 7, while five were associated with panicle length on chromosomes 2, 3, and 4. Seven SNPs were linked to thousand-grain weight on chromosomes 2, 3, and 6. The putative SNP markers associated with a low number of emerged Sh and agronomic traits in the assessed genotypes are valuable genomic resources for accelerated breeding and variety deployment of pearl millet with Sh resistance and farmer- and market-preferred agronomic traits.

High-Yielding Varieties Striga-Resistant Agronomic Parameters CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA GENOME-WIDE ASSOCIATION STUDIES STRIGA HERMONTHICA PEARL MILLET

Combination of linkage and association mapping with genomic prediction to infer QTL regions associated with gray leaf spot and northern corn leaf blight resistance in tropical maize

David Berger Yoseph Beyene Collins Juma Suresh L.M. Manje Gowda (2023, [Artículo])

Gray Leaf Spot Northern Corn Leaf Blight CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA MAIZE LEAF SPOTS QUANTITATIVE TRAIT LOCI ASSOCIATION MAPPING GENOME-WIDE ASSOCIATION STUDIES

Modular ontology to support manufacturing SMEs toward industry 4.0

ZAIDA ANTONIETA MORA ALVAREZ OSCAR HERNANDEZ URIBE RAMON ALBERTO LUQUE MORALES LEONOR ADRIANA CARDENAS ROBLEDO (2023, [Artículo])

Industry 4.0 (I4.0) implementation is a hot topic among manufacturing organizations to reach smart factory status and integrate a fully connected ecosystem. Achieving such a transition presents notable challenges for Small and Medium Enterprises (SMEs) since they often face resource and skilled personnel limitations. This study developed a domain ontology to represent various stages of maturity toward I4.0 implementation. Ontology provides a tool for SMEs to self-assess in situations of machines, processes, and factories for the dimensions of control, integration, and intelligence. This study focused on the identification of classes and relationships according to I4.0 implementation situations in the context of a manufacturing setting, the reuse of ontologies related to the domain of observations to model situations, and the creation and validation of the ontology through the information obtained from the questionnaires applied to SMEs. Finally, the ontology delivers a tool to understand SMEs' current state concerning I4.0 implementation and plan based on informed decisions about the maturity state and the technology required to advance to the next stage in their manufacturing processes.

This study was partially supported by the grants CONAHCYT-CIATEQ CVU 899567 and 162867 and CONAHCYT SNI.

We express our gratitude to Teresa Novales Hernandez for the library support.

Domain ontology Industry 4.0 SMEs Smart factory SPARQL Semantic web INGENIERÍA Y TECNOLOGÍA CIENCIAS TECNOLÓGICAS OTRAS ESPECIALIDADES TECNOLÓGICAS OTRAS OTRAS

Farmers′ use of climate change adaptation strategies and their impacts on food security in Kenya

Girma Gezmu Gebre Yuichiro Amekawa Asmiro Abeje Fikadu Dil Bahadur Rahut (2023, [Artículo])

Adaptation Strategies Smallholder Farmers CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA CLIMATE CHANGE FOOD SECURITY SMALLHOLDERS

Training Manual for Two-wheel tractor and ancillary equipment for operators, service providers, extension experts and workshop owners

Rabe Yahaya (2022, [Libro])

Mechanization is a term used to describe tools, implements and machinery applied to improve the productivity of farm land and labour force, including crop processing after harvest. Mechanization covers broadly the entire process of on and off farm operations and mechanization may use either human, animal or motorized power, or a combination of these. In practice, therefore, it involves the provision and use of all forms of power sources and mechanical assistance to agriculture, from simple hand tools to draught animal power and to motorized power technologies. In Ethiopia, smallholder farming experiences high drudgery at all stages of crop husbandry and post-harvest processing. Field operations are performed using human and animal power (Mrema et al., 2008). Smallholder farmers without animal power use a lot of human power on crop husbandry and harvesting operations (Daum et al., 2020). Postharvest threshing and shelling are performed using human power but, in some countries like Ethiopia, farmers use livestock (cattle, donkeys and horses) for wheat, barley and teff threshing (Mohammed and Tadesse, 2018). The use of tractor power is low in Ethiopia and the government aims to increase farm power available to Ethiopian farmers 10 folds (from the current 0.1 Kw ha-1 to 1 kW ha-1 ) by 2025, with at least half of this power is derived from fossil fuel and electric engines. The government of Ethiopia aims to increase farm power using two-wheel tractors on smallholder farms in addition to four-wheel tractors that are already in use. Two-wheel tractors are sources of power designed to perform most field operations. Due to the size of two-wheel tractors, they have become an economic alternative for smallholder farming. In addition, twowheel tractors are also more productive than animal traction and they require less time for attendance and preparation, giving the individual farmer more independence and contact with modern technology. Also, due to their simple design, local manufacturing of two-wheel tractors has been implemented in several countries successfully, increasing employment opportunities in the process.

CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA FARM EQUIPMENT TRACTORS TRAINING SMALLHOLDERS

Evaluation of multiple satellite precipitation products for rainfed maize production systems over Vietnam

Paresh Shirsath Dakshina Murthy Kadiyala (2022, [Artículo])

Rainfall Datasets Satellite Rainfall Estimates CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA RAIN RAINFED FARMING DATA SATELLITES

Author Correction: genome-wide association analyses of agronomic traits and Striga hermonthica resistance in pearl millet

Hussein Shimelis Chris Ojiewo Abhishek Rathore (2023, [Artículo])

Agronomic Traits CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA GENOME-WIDE ASSOCIATION STUDIES STRIGA HERMONTHICA PEARL MILLET

Genome-wide association analysis of tan spot disease resistance in durum wheat accessions from Tunisia

marwa laribi Sarrah Ben M'barek Carolina Sansaloni Susanne Dreisigacker (2023, [Artículo])

CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA DISEASE RESISTANCE HARD WHEAT GENETIC DIVERSITY GENOME-WIDE ASSOCIATION STUDIES LANDRACES POPULATION STRUCTURE

Análisis de las conexiones matemáticas en la enseñanza y aprendizaje de la derivada basado en un networking of theories entre la Teoría de las conexiones y el Enfoque ontosemiótico.

CAMILO ANDRES RODRIGUEZ NIETO (2021, [Tesis de doctorado])

Consejo Nacional de Ciencia y Tecnología No. 602990

In research in Mathematics Education, models have been reported to analyze mathematical connections in which specific connection categories are considered. In the literature, it was identified that the most used model is the Businskas with contributions from other researchers. However, the problem refers to the fact that some categories of connections limit the analysis of mathematical activity and, therefore, the research suggests that the established categories are validated and, if possible, new categories of connections are reported. Other investigations focused on exploring mathematical connections and understanding the derivative reveal that high school students, pre-service teachers, and some in-service mathematics teachers have difficulty connecting multiple representations of the derivative (e.g., algebraic, or symbolic, verbal, graphic, tabular) and establish connections between partial meanings about this concept.

Networking of theories Mathematical connections Onto-semiotic approach semiotic function derivative teacher students HUMANIDADES Y CIENCIAS DE LA CONDUCTA PEDAGOGÍA TEORÍA Y MÉTODOS EDUCATIVOS TEORÍAS EDUCATIVAS

Desarrollo de nanocompositos de cápside viral P22 para la terapia con profármacos enzimáticos en cáncer de mama

Development of P22 viral capsid nanocomposites for enzymatic prodrug therapy in breast cancer

Astrid Rebeca Luna Rios (2024, [Tesis de maestría])

Las estrategias actuales contra el cáncer de mama presentan limitaciones debido a la falta de selectividad, disminuyendo su efectividad. La mayoría de los fármacos, como el tamoxifeno, requieren la activación metabólica por parte de las enzimas de la familia citocromo P450 (CYP) para ejercer mayores efectos anticancerígenos. Sin embargo, la concentración del CYP es variable y baja en las células tumorales, especialmente en el cáncer de mama, lo que provoca efectos secundarios. En la terapia de activación enzimática de profármacos (EPT), las enzimas se dirigen al tumor para transformar el profármaco, lo que normalmente implica la administración de la enzima seguida del profármaco. Sin embargo, las diferencias entre la farmacocinética y farmacodinamia son un obstáculo para mejorar el tratamiento. Por lo tanto, la administración conjunta de profármaco y enzima es esencial para garantizar su interacción favorable en el tumor. Este trabajo reporta una nueva clase de nanocompositos terapéuticos basados en partículas tipo virus P22 confinando CYP, funcionalizadas superficialmente con glucosa oxidasa (GOx) que transforma la glucosa en D-glucono-δ-lactona produciendo H2O2, el aceptor final de electrones en la transformación de tamoxifeno mediado por CYP, y conjugadas con un derivado de tamoxifeno como profármaco y ligando dirigido utilizando polietilenglicol como conector. En un microambiente tumoral rico en glucosa, estos nanocompuestos pueden producir fármaco activo in situ. Se caracterizaron las propiedades fisicoquímicas y la catálisis secuencial mediada por glucosa de los nanocompositos. Los estudios in vitro demostraron una disminución en la viabilidad celular en líneas celulares de cáncer de mama ER+ y ER-. Sin embargo, la internalización celular en ausencia de glucosa mostró ser preferencial con las VLPs dirigidas en ambas líneas celulares. La administración conjunta de enzimas y profármacos con una localización mejorada de las VLPs desarrolladas después de la funcionalización del tamoxifeno, lo que sugiere el potencial de los nanocompositos desarrollados para superar los desafíos existentes de la EPT y mejorar los resultados terapéuticos con efectos secundarios reducidos.

Current treatment strategies against breast cancer have limitations due to lack in selectivity. Most drugs, such as tamoxifen, require metabolic activation by cytochrome P450 (CYP) enzymes to perform greater anticancer effects. However, the concentration of CYP varies and is low in tumor cells, especially in breast cancer, resulting in side-effects. In enzyme prodrug therapy (EPT), enzymes are targeted to the tumor cells for prodrug transformation, typically involving the sequential delivery of the enzyme followed by the prodrug. However, differences in pharmacokinetics and pharmacodynamics are a major hindrance for improving treatment. Therefore, co-delivery of prodrug and enzyme is essential to ensure their favorable interaction in tumor. This work reports a new class of therapeutic nanocomposites based on P22 virus like particles (VLPs) confining the CYP activity, surface functionalized with glucose oxidase (GOx) that transforms glucose into D-glucono-δ-lactone producing hydrogen peroxide, the final electron acceptor in the CYP-mediated transformation of tamoxifen, and together conjugated with a tamoxifen derivative as prodrug and targeting ligand using polyethylene glycol as a linker. In glucose-rich tumor microenvironment, these nanocomposites can produce active drug in situ. The physicochemical properties and sequential glucose-mediated catalysis of the nanocomposites were characterized. In vitro studies demonstrated a decrease in cell viability in both ER+ and ER- breast cancer cell lines. However, cellular internalization in the absence of glucose showed improved uptake of targeted VLPs in both cell lines. The co-delivery of enzymes and prodrug with improved localization of developed VLPs after tamoxifen functionalization, suggests the potential of developed nanocomposites to overcome the existing challenges of EPT and improve the therapeutic outcomes with reduced side effects.

VLP-P22, Citocromo P45O, Glucosa oxidasa, Tamoxifeno, Terapia de activación enzimática de profármacos VLP-P22, Cytochrome P45O, Glucose oxidase, Tamoxifen, Enzyme prodrug therapy BIOLOGÍA Y QUÍMICA QUÍMICA QUÍMICA FARMACÉUTICA DISEÑO.SÍNTESIS Y ESTUDIO NUEVOS FÁRMACOS DISEÑO.SÍNTESIS Y ESTUDIO NUEVOS FÁRMACOS