Repositorio Nacional

Coronin plays a major role in the organization and dynamics of actin in yeast. To investigate the role of coronin in a filamentous fungus (Neurospora crassa), we examined its subcellular localization using fluorescent proteins and the phenotypic consequences of coronin gene (crn-1) deletion in hyphal morphogenesis, Spitzenkörper behavior and endocytosis. Coronin-GFP was localized in patches, forming a subapical collar near the hyphal apex; significantly, it was absent from the apex. The subapical patches of coronin colocalized with fimbrin, Arp2/3 complex, and actin, altogether comprising the endocytic collar. Deletion of crn-1 resulted in reduced hyphal growth rates, distorted hyphal morphology, uneven wall thickness, and delayed establishment of polarity during germination; it also affected growth directionality and increased branching. The Spitzenkörper of Δcrn-1 mutant was unstable; it appeared and disappeared intermittently giving rise to periods of hyphoid-like and isotropic growth respectively. Uptake of FM4-64 in Δcrn-1 mutant indicated a partial disruption in endocytosis. These observations underscore coronin as an important component of F-actin remodeling in N. crassa. Although coronin is not essential in this fungus, its deletion influenced negatively the operation of the actin cytoskeleton involved in the orderly deployment of the apical growth apparatus, thus preventing normal hyphal growth and morphogenesis. © 2012 Echauri-Espinosa et al.

Using homosoils for quantitative extrapolation of soil mapping models

Andree Nenkam Alexandre Wadoux Budiman Minasny Alex McBratney Pierre C. Sibiry Traore Gatien Falconnier Anthony Whitbread (2022, [Artículo])

Cubist Digital Soil Mapping Model-Based Validation Soil Spatial Variation Soil-Forming Factors CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA LAND USE ORGANIC CARBON SOIL SURVEYS SPATIAL VARIATIONS

Effect of antimicrobial nanocomposites on Vibrio cholerae lifestyles: Pellicle biofilm, planktonic and surface-attached biofilm

ANAID MEZA VILLEZCAS (2019, [Artículo])

Vibrio cholerae is an important human pathogen causing intestinal disease with a high incidence in developing countries. V. cholerae can switch between planktonic and biofilm lifestyles. Biofilm formation is determinant for transmission, virulence and antibiotic resistance. Due to the enhanced antibiotic resistance observed by bacterial pathogens, antimicrobial nanomaterials have been used to combat infections by stopping bacterial growth and preventing biofilm formation. In this study, the effect of the nanocomposites zeolite-embedded silver (Ag), copper (Cu), or zinc (Zn) nanoparticles (NPs) was evaluated in V. cholerae planktonic cells, and in two biofilm states: pellicle biofilm (PB), formed between air-liquid interphase, and surface-attached biofilm (SB), formed at solid-liquid interfaces. Each nanocomposite type had a distinctive antimicrobial effect altering each V. cholerae lifestyles differently. The ZEO-AgNPs nanocomposite inhibited PB formation at 4 μg/ml, and prevented SB formation and eliminated planktonic cells at 8 μg/ml. In contrast, the nanocomposites ZEO-CuNPs and ZEO-ZnNPs affect V. cholerae viability but did not completely avoid bacterial growth. At transcriptional level, depending on the nanoparticles and biofilm type, nanocomposites modified the relative expression of the vpsL, rbmA and bap1, genes involved in biofilm formation. Furthermore, the relative abundance of the outer membrane proteins OmpT, OmpU, OmpA and OmpW also differs among treatments in PB and SB. This work provides a basis for further study of the nanomaterials effect at structural, genetic and proteomic levels to understand the response mechanisms of V. cholerae against metallic nanoparticles. © 2019 Meza-Villezcas et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

bacterial protein, copper nanoparticle, nanocomposite, OmpT protein, OmpU protein, OmpW protein, outer membrane protein A, silver nanoparticle, unclassified drug, zeolite, zinc nanoparticle, antiinfective agent, copper, metal nanoparticle, nanocompos BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA MICROBIOLOGÍA MICROBIOLOGÍA

Ajustar las condiciones de operación de una prensa hidráulica horizontal de fabricación propia para un proceso de doblado en frío a 90°

Hugo Estrada Pimentel HUGO ARCOS GUTIERREZ JOSE ANTONIO BETANCOURT CANTERA Jan Mayén Chaires John Edison García Herrera (2023, [Artículo])

During the cold-bending process, the tooling suffers considerable damage due to excess pressure. This also causes the machines to break down, causing problems in the precision and quality of the metal parts formed. The precision depends on the operating conditions of the press, the tooling employed, and the elastic recovery effect of the material. This study determines the working conditions for a made-in-house horizontal hydraulic press through an experimental design (DOE). This research carried out the V-forming to 90° (ISO 2768-1) of a hot-rolled carbon steel plate, considering pressure, piston permanence time, and recovery factor (Kr). The experimental and statistical analysis ensures accurate forming while the work pressure decreases by 17% and 33%, respectively, regarding the maximum. This reduction will delay the appearance of fatigue damage and have the operating parameters well established; in turn, it will be possible to design tools according to commercial standards.

Durante el proceso de conformado en frío, las herramientas sufren daños considerables debido al exceso de presión. Esto también causa que las máquinas se rompan, causando problemas en la precisión y calidad de las piezas metálicas formadas. La precisión depende de las condiciones de funcionamiento de la prensa, las herramientas empleadas y el efecto elástico de recuperación del material. Este estudio determina las condiciones de trabajo de una prensa hidráulica horizontal fabricado en casa a través de un diseño experimental (DOE). Esta investigación llevó a cabo el conformado en V a 90° (ISO 2768-1) de una placa de acero al carbono laminada en caliente, teniendo en cuenta la presión, el tiempo de permanencia del pistón y el factor de recuperación (Kr). El análisis experimental y estadístico asegura un conformado preciso, mientras que la presión de trabajo disminuyó en un 17% y 33%, respectivamente, con respecto al máximo. Esta reducción retrasará la aparición de daños por fatiga y tendrá los parámetros de funcionamiento bien establecidos; con estos, a su vez, será posible diseñar herramientas de acuerdo con los estándares comerciales.

Authorship acknowledgement: Hugo Estrada: Conceptualization; methodology; validation; writing; revision; project administration; software; investigation; display; draft writing; reviewing and editing. Hugo Arcos: Conceptualization; methodology; validation; writing; revision; simulation; software; reviewing. José A. Betancourt: Supervision; methodology; validation; writing; revision; draft writing: writing reviewing and editing. Jan Mayen: Supervision; methodology; validation; writing; revision; formal analysis.

John E. García: Conceptualization; supervision; methodology; validation; writing; revision; project management; formal analysis, draft writing: writing reviewing and editing.

Cold forming DOE ANOVA Recovery factor (Kr) Factorial analysis 2k Spring back (SB) Conformado en frío Factor de recuperación (Kr) Análisis factorial 2k Recuperación elástica (SB) INGENIERÍA Y TECNOLOGÍA CIENCIAS TECNOLÓGICAS OTRAS ESPECIALIDADES TECNOLÓGICAS OTRAS OTRAS

Assessment of resistance to cereal cyst nematodes, Heterodera avenae in Moroccan durum and bread wheat elite breeding lines

Abdelfattah DABABAT fouad mokrini (2023, [Artículo])

Durum Wheat Bread Wheat Cereal Cyst Nematodes Nematode Reproduction Factors CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA HETERODERA AVENAE HARD WHEAT SOFT WHEAT NEMATODES RESISTANCE VARIETIES SUSTAINABLE PRODUCTION CEREALS

Comparative live-cell imaging analyses of SPA-2, BUD-6 and BNI-1 in Neurospora crassa reveal novel features of the filamentous fungal polarisome

Alexander Lichius (2012, [Artículo])

A key multiprotein complex involved in regulating the actin cytoskeleton and secretory machinery required for polarized growth in fungi, is the polarisome. Recognized core constituents in budding yeast are the proteins Spa2, Pea2, Aip3/Bud6, and the key effector Bni1. Multicellular fungi display a more complex polarized morphogenesis than yeasts, suggesting that the filamentous fungal polarisome might fulfill additional functions. In this study, we compared the subcellular organization and dynamics of the putative polarisome components BUD-6 and BNI-1 with those of the bona fide polarisome marker SPA-2 at various developmental stages of Neurospora crassa. All three proteins exhibited a yeast-like polarisome configuration during polarized germ tube growth, cell fusion, septal pore plugging and tip repolarization. However, the localization patterns of all three proteins showed spatiotemporally distinct characteristics during the establishment of new polar axes, septum formation and cytokinesis, and maintained hyphal tip growth. Most notably, in vegetative hyphal tips BUD-6 accumulated as a subapical cloud excluded from the Spitzenkörper (Spk), whereas BNI-1 and SPA-2 partially colocalized with the Spk and the tip apex. Novel roles during septal plugging and cytokinesis, connected to the reinitiation of tip growth upon physical injury and conidial maturation, were identified for BUD-6 and BNI-1, respectively. Phenotypic analyses of gene deletion mutants revealed additional functions for BUD-6 and BNI-1 in cell fusion regulation, and the maintenance of Spk integrity. Considered together, our findings reveal novel polarisome-independent functions of BUD-6 and BNI-1 in Neurospora, but also suggest that all three proteins cooperate at plugged septal pores, and their complex arrangement within the apical dome of mature hypha might represent a novel aspect of filamentous fungal polarisome architecture. © 2012 Lichius et al.

fungal protein, protein BNI 1, protein BUD 6, protein SPA 2, protein Spk, unclassified drug, actin binding protein, cytoskeleton protein, fungal protein, article, cell fusion, cellular distribution, comparative study, conidium, controlled study, cyto BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA MICROBIOLOGÍA MICROBIOLOGÍA

Molecular modeling simulation studies reveal new potential inhibitors against HPV E6 protein

Joel Ricci-Lopez (2019, [Artículo])

High-risk strains of human papillomavirus (HPV) have been identified as the etiologic agent of some anogenital tract, head, and neck cancers. Although prophylactic HPV vaccines have been approved; it is still necessary a drug-based treatment against the infection and its oncogenic effects. The E6 oncoprotein is one of the most studied therapeutic targets of HPV, it has been identified as a key factor in cell immortalization and tumor progression in HPV-positive cells. E6 can promote the degradation of p53, a tumor suppressor protein, through the interaction with the cellular ubiquitin ligase E6AP. Therefore, preventing the formation of the E6-E6AP complex is one of the main strategies to inhibit the viability and proliferation of infected cells. Herein, we propose an in silico pipeline to identify small-molecule inhibitors of the E6-E6AP interaction. Virtual screening was carried out by predicting the ADME properties of the molecules and performing ensemble-based docking simulations to E6 protein followed by binding free energy estimation through MM/PB(GB)SA methods. Finally, the top-three compounds were selected, and their stability in the E6 docked complex and their effect in the inhibition of the E6-E6AP interaction was corroborated by molecular dynamics simulation. Therefore, this pipeline and the identified molecules represent a new starting point in the development of anti-HPV drugs. © 2019 Ricci-López et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

ligand, luteolin, protein E6, protein inhibitor, ubiquitin protein ligase, ubiquitin protein ligase E6AP, unclassified drug, antivirus agent, DNA binding protein, E6 protein, Human papillomavirus type 18, oncoprotein, protein binding, protein p53, TP CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA

An efficient transformation method for genome editing of elite bread wheat cultivars

Akshaya Biswal Kanwarpal Dhugga (2023, [Artículo])

Particle Bombardment Wheat Growth-Regulating Factor 4 Wheat Growth-Interacting Factor Wheat Mildew Locus O CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA CRISPR GENE EDITING TRANSFORMATION WHEAT