Repositorio Nacional

Se desarrolló y validó una metodología para la cuantificación de ácido clofíbricoderivatizado con trimetilsilildiazometano en muestras de agua por cromatografía de gases acoplada a un detector selectivo de masas (CG-MS). El método se validó en diferentes parámetros como linealidad, exactitud, precisión, límites de detección y cuantificación. Las concentraciones de validación se encuentran en el intervalo de 0.0025 a 0.1644 μgL-1. Los límites de detección y cuantificación obtenidos son 0.0003 y 0.0053 μgL-1.

Contaminación del agua Industria farmacéutica Ácido clofíbrico INGENIERÍA Y TECNOLOGÍA

Estimating lime requirements for tropical soils: Model comparison and development

Fernando Aramburu Merlos João Vasco Silva Frédéric Baudron Robert Hijmans (2023, [Artículo])

Acid tropical soils may become more productive when treated with agricultural lime, but optimal lime rates have yet to be determined in many tropical regions. In these regions, lime rates can be estimated with lime requirement models based on widely available soil data. We reviewed seven of these models and introduced a new model (LiTAS). We evaluated the models’ ability to predict the amount of lime needed to reach a target change in soil chemical properties with data from four soil incubation studies covering 31 soil types. Two foundational models, one targeting acidity saturation and the other targeting base saturation, were more accurate than the five models that were derived from them, while the LiTAS model was the most accurate. The models were used to estimate lime requirements for 303 African soil samples. We found large differences in the estimated lime rates depending on the target soil chemical property of the model. Therefore, an important first step in formulating liming recommendations is to clearly identify the soil property of interest and the target value that needs to be reached. While the LiTAS model can be useful for strategic research, more information on acidity-related problems other than aluminum toxicity is needed to comprehensively assess the benefits of liming.

Exchangeable Acidity Aluminum Saturation Calcium Carbonate Equivalent CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA CHEMICOPHYSICAL PROPERTIES LIMES TROPICAL ZONES ACID SOILS ALUMINIUM BASE SATURATION CALCIUM CARBONATE

The Banana MaWRKY18, MaWRKY45, MaWRKY60 and MaWRKY70 Genes Encode Functional Transcription Factors and Display Differential Expression in Response to Defense Phytohormones

SERGIO GARCIA LAYNES VIRGINIA AURORA HERRERA VALENCIA Lilia Guadalupe Tamayo Torres VERONICA LIMONES BRIONES FELIPE ALONSO BARREDO POOL FRAY MARTIN BAAS ESPINOLA Ángel Gabriel Alpuche Solís CARLOS ALBERTO PUCH HAU SANTY PERAZA ECHEVERRIA (2022, [Artículo])

"WRKY transcription factors (TFs) play key roles in plant defense responses through phytohormone signaling pathways. However, their functions in tropical fruit crops, especially in banana, remain largely unknown. Several WRKY genes from the model plants rice (OsWRKY45) and Arabidopsis (AtWRKY18, AtWRKY60, AtWRKY70) have shown to be attractive TFs for engineering disease resistance. In this study, we isolated four banana cDNAs (MaWRKY18, MaWRKY45, MaWRKY60, and MaWRKY70) with homology to these rice and Arabidopsis WRKY genes. The MaWRKY cDNAs were isolated from the wild banana Musa acuminata ssp. malaccensis, which is resistant to several diseases of this crop and is a progenitor of most banana cultivars. The deduced amino acid sequences of the four MaWRKY cDNAs revealed the presence of the conserved WRKY domain of ~60 amino acids and a zinc-finger motif at the N-terminus. Based on the number of WRKY repeats and the structure of the zinc-finger motif, MaWRKY18 and MaWRKY60 belong to group II of WRKY TFs, while MaWRKY45 and MaWRKY70 are members of group III. Their corresponding proteins were located in the nuclei of onion epidermal cells and were shown to be functional TFs in yeast cells. Moreover, expression analyses revealed that the majority of these MaWRKY genes were upregulated by salicylic acid (SA) or methyl jasmonate (MeJA) phytohormones, although the expression levels were relatively higher with MeJA treatment. The fact that most of these banana WRKY genes were upregulated by SA or MeJA, which are involved in systemic acquired resistance (SAR) or induced systemic resistance (ISR), respectively, make them interesting candidates for bioengineering broad-spectrum resistance in this crop."

Banana Transcription factor WRKY Defense phytohormones Salicylic acid Methyl jasmonate SAR ISR Broad-spectrum resistance BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA GENÉTICA GENÉTICA

ANÁLISIS DE PIGMENTOS, PEROXIDASA, PROLINA Y PROTEÍNAS DE TRES ESPECIES DE Paulownia BAJO ESTRES HÍDRICO

ANALYSIS OF PIGMENTS, PEROXIDASE, PROLINE AND PROTEINS OF THREE Paulownia SPECIES UNDER WATER STRESS

José Manuel Llano Sotelo Lilia Alcaráz Meléndez (2012, [Artículo])

"Los árboles del género Paulownia son de rápido crecimiento y tienen importancia económica en Asia como materia prima de uso común en la elaboración de muebles, instrumentos musicales y cercos, lo que ha despertado interés por cultivarlos en diferentes ambientes. Con el objetivo de evaluar la tolerancia al estrés hídrico se eligieron tres especies, P. imperialis, P. fortunei y P. elongata, porque son las que se utilizan en China con mayor frecuencia en la reforestación y en la industria maderera. Se realizaron análisis bioquímicos de las hojas para determinar los siguientes componentes: como pigmentos, a las clorofilas total, a y b; β-caroteno, violaxantina y luteína); las enzimas peroxidasa y prolina, así como proteínas solubles, insolubles y totales en tres diferentes condiciones de humedad del suelo, bajo dos condiciones ambientales, campo e invernadero, entre los cuales se observaron diferencias significativas; destaca un incremento del contenido de pigmentos y peroxidasa, y una disminución de las proteínas y prolina en el ambiente controlado, principalmente. Al evaluar la respuesta al estrés hídrico entre las especies se concluyó que P. imperialis y P. elongata son más tolerantes que P. fortunei, debido a su mayor contenido de prolina, mas proteínas totales y solubles, indicadores de una mejor tolerancia a condiciones de estrés."

"Paulownia trees are fast growing and are economically important in Asia as raw material regularly used for furniture, musical instruments, fences, etc., which rose some interest in their cultivation in different environments. In order to assess water stress tolerance, three of these species, P. imperialis, P. fortunei and P. elongate, were selected as they are the most commonly used in reforestation and wood industry in China. Biochemical analyses were performed in leaves, to determine the following elements: pigments (total, a and b chlorophyll; β-carotene, violaxanthin; lutein), peroxidase, proline, total, soluble and insoluble proteins, under three moisture soil concentrations and two environments, field and greenhouse, the latter of which revealed significant differences between them; there is an increment in pigment and peroxidase content and a decrease in protein and proline content, mainly in the greenhouse environment. When water stress was assessed among species, it was concluded that P. imperialis and P. elongata are more tolerant than P. fortunei because of their higher content of proline, total and soluble proteins, which are major tolerance markers in stress conditions. "

Aminoácidos, luteína, Paulownia elongata S. Y. Hu, Paulownia fortunei (Seem.) Hemsl., Paulownia imperialis Siebold & Zucc., peroxidasa, violaxantina Amino acids BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA BIOLOGÍA VEGETAL (BOTÁNICA) FISIOLOGÍA VEGETAL FISIOLOGÍA VEGETAL