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Modification of AtGRDP1 gene expression affects silique and seed development in Arabidopsis thaliana

AIDA ARACELI RODRIGUEZ HERNANDEZ CARLOS VLADIMIR MURO MEDINA JOCELIN ITZEL RAMIREZ ALONSO JUAN FRANCISCO JIMENEZ BREMONT (2017)

"Glycine Rich Proteins (GRPs) are induced at different developmental stages and in specific plant tissues. Recently, we described a novel Arabidopsis gene encoding a short glycine-rich domain protein (AtGRDP1). This gene is involved in abiotic stress responsiveness; the Atgrdp1-null mutant seeds were more sensitive to stress, while the opposite phenotype was achieved by AtGRDP1 overexpression. In this study, we analyzed the phenotype of the fruits produced by Arabidopsis Atgrdp1 mutants and 35S::AtGRDP1 overexpression lines. Our analyses revealed important changes in silique length, seed number, seed weight and morphology in the analyzed lines. In particular, Atgrdp1 mutant lines exhibited several defects including short siliques, a diminished number of seeds per silique, and a reduction in seed size and weight as compared to Col-0. The overexpression of the AtGRDP1 gene also generated phenotypes with alterations in size of silique, number of seeds per silique, and size and weight of the seed. In addition, the expression analysis of AtGRDP1 gene showed that it was expressed in floral and fruit organs, with the highest expression level in mature siliques. The alterations in the siliques and seeds traits in the Atgrdp1 mutant line, as well as the phenotypes observed in AtGRDP1 overexpression lines, suggest a role of the AtGRDP1 gene in the Arabidopsis fruit development."

Article

AtGRDP1 Glycine-rich domain protein Seeds Siliques BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA BIOLOGÍA MOLECULAR

Overexpression of AtGRDP2, a novel glycine-rich domain protein, accelerates plant growth and improves stress tolerance

MARIA AZUCENA ORTEGA AMARO AIDA ARACELI RODRIGUEZ HERNANDEZ MARGARITA RODRIGUEZ Y DOMINGUEZ KESSLER ELOISA HERNANDEZ LUCERO SERGIO ROSALES MENDOZA ALEJANDRO IBAÑEZ SALAZAR PABLO DELGADO SANCHEZ JUAN FRANCISCO JIMENEZ BREMONT (2015)

"Proteins with glycine-rich signatures have been reported in a wide variety of organisms including plants, mammalians, fungi, and bacteria. Plant glycine-rich protein genes exhibit developmental and tissue-specific expression patterns. Herein, we present the characterization of the AtGRDP2 gene using Arabidopsis null and knockdown mutants and, Arabidopsis and lettuce over-expression lines. AtGRDP2 encodes a short glycine-rich domain protein, containing a DUF1399 domain and a putative RNA recognition motif (RRM). AtGRDP2 transcript is mainly expressed in Arabidopsis floral organs, and its deregulation in Arabidopsis Atgrdp2 mutants and 35S::AtGRDP2 over-expression lines produces alterations in development. The 35S::AtGRDP2 over-expression lines grow faster than the WT, while the Atgrdp2 mutants have a delay in growth and development. The over-expression lines accumulate higher levels of indole-3-acetic acid and, have alterations in the expression pattern of ARF6, ARF8, and miR167 regulators of floral development and auxin signaling. Under salt stress conditions, 35S::AtGRDP2 over-expression lines displayed higher tolerance and increased expression of stress marker genes. Likewise, transgenic lettuce plants over-expressing the AtGRDP2 gene manifest increased growth rate and early flowering time. Our data reveal an important role for AtGRDP2 in Arabidopsis development and stress response, and suggest a connection between AtGRDP2 and auxin signaling."

Article

Glycine-rich domain protein Arabidopsis thaliana Lactuca sativa Development Indole-3-acetic acid Salt stress BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA

Modification of AtGRDP1 gene expression affects silique and seed development in Arabidopsis thaliana

AIDA ARACELI RODRIGUEZ HERNANDEZ CARLOS VLADIMIR MURO MEDINA JOCELIN ITZEL RAMIREZ ALONSO JUAN FRANCISCO JIMENEZ BREMONT (2017)

"Glycine Rich Proteins (GRPs) are induced at different developmental stages and in specific plant tissues. Recently, we described a novel Arabidopsis gene encoding a short glycine-rich domain protein (AtGRDP1). This gene is involved in abiotic stress responsiveness; the Atgrdp1-null mutant seeds were more sensitive to stress, while the opposite phenotype was achieved by AtGRDP1 overexpression. In this study, we analyzed the phenotype of the fruits produced by Arabidopsis Atgrdp1 mutants and 35S::AtGRDP1 overexpression lines. Our analyses revealed important changes in silique length, seed number, seed weight and morphology in the analyzed lines. In particular, Atgrdp1 mutant lines exhibited several defects including short siliques, a diminished number of seeds per silique, and a reduction in seed size and weight as compared to Col-0. The overexpression of the AtGRDP1 gene also generated phenotypes with alterations in size of silique, number of seeds per silique, and size and weight of the seed. In addition, the expression analysis of AtGRDP1 gene showed that it was expressed in floral and fruit organs, with the highest expression level in mature siliques. The alterations in the siliques and seeds traits in the Atgrdp1 mutant line, as well as the phenotypes observed in AtGRDP1 overexpression lines, suggest a role of the AtGRDP1 gene in the Arabidopsis fruit development."

Article

AtGRDP1 Glycine-rich domain protein Seeds Siliques BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA BIOLOGÍA MOLECULAR BIOLOGÍA MOLECULAR

Modification of AtGRDP1 gene expression affects silique and seed development in Arabidopsis thaliana

Aída Araceli Rodríguez Hernández CARLOS VLADIMIR MURO MEDINA Jocelin Itzel Ramíez Alonso JUAN FRANCISCO JIMENEZ BREMONT (2017)

"Glycine Rich Proteins (GRPs) are induced at different developmental stages and in specific plant tissues. Recently, we described a novel Arabidopsis gene encoding a short glycine-rich domain protein (AtGRDP1). This gene is involved in abiotic stress responsiveness; the Atgrdp1-null mutant seeds were more sensitive to stress, while the opposite phenotype was achieved by AtGRDP1 overexpression. In this study, we analyzed the phenotype of the fruits produced by Arabidopsis Atgrdp1 mutants and 35S::AtGRDP1 overexpression lines. Our analyses revealed important changes in silique length, seed number, seed weight and morphology in the analyzed lines. In particular, Atgrdp1 mutant lines exhibited several defects including short siliques, a diminished number of seeds per silique, and a reduction in seed size and weight as compared to Col-0. The overexpression of the AtGRDP1 gene also generated phenotypes with alterations in size of silique, number of seeds per silique, and size and weight of the seed. In addition, the expression analysis of AtGRDP1 gene showed that it was expressed in floral and fruit organs, with the highest expression level in mature siliques. The alterations in the siliques and seeds traits in the Atgrdp1 mutant line, as well as the phenotypes observed in AtGRDP1 overexpression lines, suggest a role of the AtGRDP1 gene in the Arabidopsis fruit development."

Article

AtGRDP1 Glycine-rich domain protein Seeds Siliques BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA BIOLOGÍA MOLECULAR BIOLOGÍA MOLECULAR

A study of the optical properties of semi-organic crystals doped with erbium

JUDITH PARRA BERUMEN (2011)

Organic crystals have nonlinear optical effects (NLO) that make them attractive for applications in frequency conversion and optical processing. Among these we find the amino acids. glycine is the simplest of all amino acids in the crystalline form, has three different polymorphs α, β and ϒ in which the molecules exist in the dipolar form (NH3+ CH2COO-). In this work, we obtained glycine crystals doped with erbium growth by slow evaporation at room temperature. The chemical composition of the crystal was determined by Fourier transform infrared (FTIR), and Raman spectroscopy and was conducted for glycine with different concentrations of erbium. The optical absorption spectrum recorded in the wavelength range of UV-vis revealed that the crystal has good optical transparency in the range of 350 to 1100 nm. The crystalline phase was determined by X-ray diffraction and the second harmonic generation efficiency of the crystal measured by using the Kurtz Perry modified method.

Article

Glycine nonlinear second harmonic generation CIENCIAS FÍSICO MATEMÁTICAS Y CIENCIAS DE LA TIERRA FÍSICA OTRAS ESPECIALIDADES FÍSICAS OTRAS

Evidence of the Second Harmonic Generation on Semi-Organic Glycine-Erbium Nitrate

JUDITH PARRA BERUMEN PEDRO PIZA RUIZ ERASMO ORRANTIA BORUNDA JOSE ALBERTO DUARTE MOLLER (2012)

Organic crystals have nonlinear optical effects (NLO) that make them attractive for applications in frequency conversion and optical processing. Among these we find the amino acids. Glycine is the simplest of all amino acids in the crystalline form, has three different polymorphs α, β,y γ in which the molecules exist in the dipolar form (NH3+ CH2COO-). In this work we obtained glycine crystals doped with erbium growth by slow evaporation at room temperature. The chemical composition of the crystal was determined by Raman spectroscopy and was conducted for glycine with different concentrations of erbium. The optical absorption spectrum recorded in the wavelength range of UV-vis revealed that the crystal has good optical transparency in the range of 350-1100 nm. The second harmonic generation efficiency of the crystal measured by using the Kurtz Perry modified method.

Article

glycine NLO CIENCIAS FÍSICO MATEMÁTICAS Y CIENCIAS DE LA TIERRA FÍSICA OTRAS ESPECIALIDADES FÍSICAS OTRAS

Cambios bioquímicos en semillas de Lupinos montanus y Lupinus exaltatus asociados a tratamientos físicos, químicos y germinativos

BERENICE JUAREZ FUENTES (2013)

Tesis (Maestría en Ciencias, especialista en Producción Agroalimentaria en el Trópico).- Colegio de Postgraduados, 2013.

Una alternativa para mejorar el valor nutricional de Lupinus silvestres es la aplicación de métodos tradicionales de procesado a las semillas. El presente estudio cuantificó los cambios bioquímicos en semillas de Lupinus exaltatus (Le) y L. montanus (Lm), especies silvestres mexicanas después de la aplicación de tratamientos físicos (hidrotérmico, remojo y descascarillado), químico (alcalino, Na2CO3 0.05%) y germinativo, para disminuir los compuestos anti-nutricionales (FAN’s) y mantener los nutricionales (CN). Muestras masales de semillas por especie fueron colectadas en 2012, en Chalchicomula de Sesma y Tlachichuca, Puebla. Los CN analizados antes y después de los tratamientos fueron: proteína cruda (PC), fibra cruda (FC), extracto etéreo (EE), cenizas (Ce). Los FAN’s fueron: alcaloides totales (AT’s), polifenoles totales (PT), taninos totales (TT) y taninos condensados (TC). Sin tratamiento, las semillas de Le y Lm presentaron 43 y 45.9% de PC, 493.8 y 416.8 mg 100 g-1 de PT y 2.18 y 3.33% de AT’s, respectivamente. La eliminación de la testa incrementó los contenidos de PC, EE y AT’s en los cotiledones de ambas especies. El tratamiento hidrotérmico durante 6 h a 95°C redujo 82 y 62.7% los AT’s y 75.2 y 85% los PT en Le y Lm, respectivamente, e incrementaron PC y FC. Reducción de AT’s también se observó en germinados de 6 días en Le y Lm (33.5 y 35.4%, respectivamente), y después de 9 h del tratamiento alcalino (TA) en Le (31.6%). El TA también redujo los CN. La germinación aumentó 54 y 84% PT en Le y Lm, respectivamente. El tratamiento de remojo no mostró variación en los CN y disminuyó los TT solo en Le. Se concluye que el tratamiento hidrotérmico por 6 h fue más eficiente en la eliminación de FAN’s en las semillas de lupino evaluadas, pero esto no es suficiente para su consumo humano. _______________ BIOCHEMICAL CHANGES IN SEEDS OF Lupinus montanus and Lupinus exaltatus ASSOCIATED WITH PHYSICAL, CHEMICAL AND GERMINATION TREATMENTS. ABSTRACT: An alternative to improve the nutritional value of wild Lupinus is the application of traditional methods of processing their seeds. The present study evaluated the biochemical changes of wild Mexican seeds of Lupinus exaltatus (Le) and L. montanus (Lm), after application of physical treatments (hydrothermal, dehulling and soak), chemical (alkaline, Na2CO3 0.05%) and germination, to reduce anti-nutritional compounds (FAN's) and maintain the nutritional (CN). Seed bulks samples were collected, by species, on 2012 at Chalchicomula de Sesma and Tlachichuca, Puebla, Mexico. The CN analyzed before and after treatments were: crude protein (CP), crude fiber (CF), ether extract (EE), ash (Ce). The FAN's were: total alkaloids (TA's), total polyphenols (TP), total tannins (TT) and condensed tannins (CT). Seeds of Le and Lm from control treatment, showed 43 and 45.9% CP, 493.8 and 416.8 mg 100 g-1 of PT and 2.18 and 3.33% of AT's, respectively. The removal of the testa increased the contents of CP, EE and AT's in the cotyledons of both species. The hydrothermal treatment for 6 h at 95°C decreased 82 and 62.7% of AT's and 75.2 and 85% of PT in Le and Lm, respectively, and increased PC and FC. AT's reduction was also observed in the germination treatment at 6 days in Le and Lm (33.5 and 35.4%, respectively), and after 9 h of the alkaline treatment (TA) in Le (31.6%). The TA also reduced CN. Germination increased 54 and 84% PT in both species. The soaking treatment showed no variation in CN and TT decreased only in Le. It is concluded that the hydrothermal treatment for 6 h was more efficient at removing FAN's in lupine seeds evaluated, but this is not enough for human consumption.

Master thesis

Lupinus Alcaloides Proteína Tratamiento térmico y de germinación Alkaloid Heat and germination treatments Protein Producción Agroalimentaria en el Trópico Maestría CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA

Perfil de ácidos grasos en Lupinus uncinatus Schlecht

SERGIO LOREDO DAVILA (2012)

Tesis (Doctorado en Ciencias, especialista en Edafología).- Colegio de Postgraduados, 2012.

Lupinus L. (Fabaceae) es un género amplio y diverso que comprende aproximadamente 500 especies anuales y perennes, así como algunas herbáceas, arbustos y árboles pequeños que se producen en una amplia gama de condiciones ecogeográficas, tanto en el Nuevo y el Viejo Mundo. Lupinus es más diversos en el Nuevo Mundo, con más del 90% de las especies del género. Se distribuyen principalmente en las regiiones alpina, templada, subtropical y los biomas de la cordillera occidental del Nuevo Mundo, desde Alaska al sur de Argentina y Chile. Sólo 12-13 especies son nativas de la región del Mediterráneo y África. Pero de todas las especies sólo cinco son cultivadas y de estas, sólo tres son explotadas comercialmente; L. albus, L. angustifolius y L. luteus. Y se basa en su alto contenido de proteínas y bajo contenido de alcaloides. Sin embargo, poca atención se ha prestado al contenido y la calidad de su aceite. El objetivo de esta tesis fue determinar el contenido de aceite y el perfil de ácidos grasos presentes en semillas de L. uncinatus Schlecht y evaluar los cambios de estós respecto al tiempo para determinar sus posibles usos en proceso dentro de la industria. Las semillas para este experimento fueron colectadas en tres tiempos diferentes. Los objetivos de la investigación fueron 1) Determinar la relación de las semillas por tamaños (longitud y ancho) en tres periodos de tiempo (con tres años, un año y recién colectadas) de L. unicinatus Schlecht y determinar si estas tienen diferencias en composición de aceite. 2) Porcentaje de aceite y dinámica del perfil de ácidos grasos en semillas de L. unicinatus Schlecht, respecto al tiempo. 3) Caracterizar la viscosidad del aceite de las semillas de L. uncinatus Schlecht a diferentes temperatura en tres diferentes colectas con (con tres años, un año y recién colectadas). 4) Caracterizar las proteínas de la torta después de la extracción de aceite en de diferentes colectas de semillas. Los parámetros evaluados fueron tamaño de semillas (longitud, ancho y peso), porcentaje de humedad y aceite; así como cromatografía de gases para determinar el perfil de ácidos grasos presentes y viscosidad del aceite y finalmente se determino las proteínas presentes en la torta despúes de la extracción del aceite. La colecta con más tiempo fue la que presento mayor contenido de aceite, humedad baja y el perfil de ácidos grasos con un alto porcentaje de ácidos grasos poliinsaturados, viscosidad alta en coparación de las otras dos colectas. El experimento se llevo acabo en el laboratorio de Jovenes Investigadores de la preparatoria agrícola en la Universidad Autónoma Chapingo. Los resultados mostraron que el contenido de aceite esta en un rango de 7.8 a 9.6 % pero estos son diferentes en cuanto a su perfil. Asimismo, las proteínas son más abundantes cuando esta frescas las semillas pero aún así después de tres años presenta proteínas de calidad para su posible uso como un alimento, pero esto dependerá de si no existen alguna sustancia toxica para animales o el hombre. Esta especie puede considerarse como una buena alternativa para la industria alimentos, farmacéutica, jabones, pinturas, resinas, revestimiento, cosméticos, lubricantes, químicos, cubiertas plásticas, etanol y biocombustibles. _______________ FATTY ACID PROFILE FROM Lupinus uncinatus Schlecht. ABSTRACT: Lupinus L. (Fabaceae) is a large and diverse genus comprising aproximate 500 annual and perennial herbaceous species, as well as a few soft-woody shrubs and small trees which occur in a wide range of ecogeographical conditions in both the New and the Old World. Lupines are more diverse in the New World with over 90% of the species in the genus. They are mainly distributed in alpine, temperate, and subtropical biomes of the western cordillera of the New World, from Alaska to South Argentina and Chile. Only 12–13 species are native to the Mediterranean region and Africa. But of all the species only five are cultivated and of these, only three are commercially exploited: L. albus, L. angustifolius and L. luteus. And it is based on its highest protein and low concentration of alkaloids. However, little attention has been paid to content and quality of oil. The aim of this thesis was to determine the oil content and fatty acid profile present in seeds of L. uncinatus Schlecht and evaluate changes thereof over time to determine its possible uses in process of the industry. The seeds for this experiment were collected at three different times. The research objectives were to 1) determine the relationship of seed size (length and width) in three time periods (three years, one year and freshly collected) of L. unicinatus Schlecht and whether these have differences in oil composition. 2) Percentage of oil and profile of fatty acids in seeds of L. unicinatus Schlecht, versus time. 3) Characterize the viscosity of the oil from the seeds of L. uncinatus Schlecht at different temperatures in three different collections. 4) To characterize the proteins of the cake after the extraction of oil from different collections of seeds. The parameters evaluated were seed size (length, width and weight), moisture and oil and gas chromatography to determine the profile of fatty acids and oil viscosity and finally determine the proteins present in the cake after oil extraction. The seeds with more time, they have most oil content, lower humidity and the fatty acid profile with a high percentage of polyunsaturated fatty acids, higher viscosity than the others seeds colleted. The experiment was held in the laboratory for Young Researchers of the Agricultural Highschool in Universidad Autónoma Chapingo. The results showed that the oil content is in a range of 7.8 to 9.6 % but these differ in their profile. Also, proteins are more abundant when this fresh seeds yet after three years has quality protein for potential use as a food, but this depends on whether there are any substances toxic to animals or humans. This species can be considered a good alternative for food industry, pharmaceuticals, soaps, paints, resins, coatings, cosmetics, lubricants, chemicals, plastic covers, ethanol and biofuels.

Doctoral thesis

Proteínas Oleaginosas Aceite vegetal Grasas Protein Oilseed Vegetable oil Fats Edafología Doctorado CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA

A COMPARATIVE STUDY OF PHYSICO-CHEMICAL, PROXIMATE COMPOSITION AND MICROGIOLOGICAL MUSCLE PROPERTIES, IN TWO SPECIES SHRIMPS OF THE PACIFIC TROPICAL COAST

DAGOBERTO PUGA LOPEZ JESUS TRINIDAD PONCE PALAFOX GUILLERMO BARBA QUINTERO MAGDA ROSALIA TORRES HERRERA JOSE LUIS ARREDONDO FIGUEROA (2013)

Physicochemical, proximate composition and microbiological analysis of white shrimp (Litopenaeus vannamei) and blue shrimp (L. stylirostris) tissues were compared. The wild shrimp were collected off the coasts of Sinaloa, México (in front Navachiste Bay, Guasave) and of Nayarit (in front Boca de Cuautla-Novilleros, Tecuala), Mexico. The results of our study suggested that meats of white and blue shrimp are a good source of protein and lipids. The blue shrimp tend to have better protein content than white shrimp, and white shrimp tend to have better lipid content than blue shrimp.

Se compararon la composición fisicoquímica, la composición inmediata y el análisis microbiológico de los camarones blancos (Litopenaeus vannamei) y camarones azules (L. stylirostris). Los camarones salvajes fueron recolectados de las costas de Sinaloa, México (frente a la Bahía Navachiste, Guasave) y de Nayarit (frente a Boca de Cuautla-Novilleros, Tecuala), México. Los resultados de nuestro estudio sugieren que las carnes de camarón blanco y azul son una buena fuente de proteínas y lípidos. El camarón azul tiende a tener un mejor contenido de proteínas que el camarón blanco, y el camarón blanco tiende a tener un mejor contenido de lípidos que el camarón azul.

Article

Protein Lipid Faecal coliform l. vannamei Litopenaeus stylirostris Proteína Lipido Coliformes fecales CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA

Arabinoxilanos y la Relación de la Fracción Proteica Remanente con la Capacidad Gelificante del Polisacárido

Arabinoxylans and the remaining protein fraction relationship with the gelling capability of the polysaccharide

MAYRA ALEJANDRA MENDEZ ENCINAS Elizabeth Carvajal Millan Agustín Rascón Chu Yolanda Leticia López Franco Jaime Lizardi Mendoza (2019)

Los arabinoxilanos (AX) son polisacáridos no amiláceos presentes en varios tejidos de cereales. Estos polisacáridos tienen la capacidad de formar geles covalentes mediante el acoplamiento oxidativo del ácido ferúlico por acción de agentes generadores de radicales libres. La capacidad gelificante de los AX depende de las características estructurales del polisacárido, por lo que el conocimiento de estas y su efecto en las propiedades del gel es esencial para crear una comprensión profunda sobre la naturaleza de estos geles. El contenido de proteína en AX es una de estas características estructurales, cuyo impacto en la gelificación del polisacárido y en las propiedades del gel no ha sido investigado a profundidad. Hasta el momento, no se conoce cómo es que la proteína se encuentra unida al AX. Esta revisión presenta investigaciones realizadas sobre la asociación arabinoxilano-proteína y la relación de la fracción proteica con la capacidad gelificante del polisacárido, así como de la posible unión covalente en dicha asociación.

Arabinoxylans (AX) are non-starch polysaccharides present in several tissues of cereal grains. These polysaccharides can form covalent gels through oxidative coupling of ferulic acid by the action of free radical-

generating agents. Since the gelling capability of AX depends on their structural characteristics, the knowledge of structural characteristics a

nd their effect on gel properties is essential for building a thorough understanding about the nature of these gels. Protein content of AX is one of such structural characteristics, whose impact on the gelling of the polysaccharide and gel properties has not been investigated in detail. Till date, it is not known how the protein is attached to the AX. This review presents research done on the arabinoxylan-protein association and the relationship of the protein fraction with the gelling capability of the polysaccharide, as well as the possible covalent binding on that association.

Article

CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA Arabinoxilano Enlace covalente Ácido Ferúlico Gelificación Proteína Arabinoxylan Covalent linkage Ferulic acid Gelling Protein