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Application of ammonium to a N limited arable soil enriches a succession of bacteria typically found in the rhizosphere

Yendi Navarro-Noya Marco Luna_Guido Nele Verhulst Bram Govaerts Luc Dendooven (2022, [Artículo])

Crop residue management and tillage are known to affect the soil bacterial community, but when and which bacterial groups are enriched by application of ammonium in soil under different agricultural practices from a semi-arid ecosystem is still poorly understood. Soil was sampled from a long-term agronomic experiment with conventional tilled beds and crop residue retention (CT treatment), permanent beds with crop residue burned (PBB treatment) or retained (PBC) left unfertilized or fertilized with 300 kg urea-N ha-1 and cultivated with wheat (Triticum durum L.)/maize (Zea mays L.) rotation. Soil samples, fertilized or unfertilized, were amended or not (control) with a solution of (NH4)2SO4 (300 kg N ha-1) and were incubated aerobically at 25 ± 2 °C for 56 days, while CO2 emission, mineral N and the bacterial community were monitored. Application of NH4+ significantly increased the C mineralization independent of tillage-residue management or N fertilizer. Oxidation of NH4+ and NO2- was faster in the fertilized soil than in the unfertilized soil. The relative abundance of Nitrosovibrio, the sole ammonium oxidizer detected, was higher in the fertilized than in the unfertilized soil; and similarly, that of Nitrospira, the sole nitrite oxidizer. Application of NH4+ enriched Pseudomonas, Flavisolibacter, Enterobacter and Pseudoxanthomonas in the first week and Rheinheimera, Acinetobacter and Achromobacter between day 7 and 28. The application of ammonium to a soil cultivated with wheat and maize enriched a sequence of bacterial genera characterized as rhizospheric and/or endophytic independent of the application of urea, retention or burning of the crop residue, or tillage.

CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA AMMONIUM CROP RESIDUES WHEAT MAIZE TILLAGE SOIL

Corrección de defectos óseos en el área de Ingeniería tisular

Correction of bone defects by tissue Engineering

ROSA ALICIA SAUCEDO ACUÑA MONICA GALICIA GARCIA JUDITH VIRGINIA RIOS ARANA SIMON YOBANNY REYES LOPEZ (2012, [Artículo])

Hoy en día, los defectos óseos representan uno de los casos de mayor impacto en la salud debido a la frecuencia con que éstos ocurren a causa de traumatismos, fracturas, enfermedades congénitas o degenerativas. En la actualidad, los implantes de tejido óseo de gran volumen se encuentran severamente restringidos a causa de las limitaciones de difusión en la interacción con el ambiente del huésped para los nutrientes, intercambio gaseoso y eliminación de desechos. Es por ello que la corrección de los defectos óseos ha cobrado gran importancia en el área de Ingeniería tisular buscando mejorar las estrategias clínicas para su tratamiento. El propósito de esta revisión es proporcionar un panorama general del desarrollo de andamios para la regeneración de tejido óseo, mostrando los avances logrados en los ensayos in vitro e in vivo en la última década

Currently, bone defects cases represent a major impact on health due to how often they occur because of trauma, fractures, congenital or degenerative diseases. Now, bone implants to large volume are severely restricted because of the diffusion limitations in the interaction

with the environment of the host for nutrients, gas exchange and waste disposal. That is why the correction of bone defects has become very important in the field of tissue engineering looking to improve clinical strategies for treatment. The purpose of this review is to provide an overview of the development of scaffolds for bone tissue regeneration, showing the progress made in the in vitro and in vivo in recent decades.

MEDICINA Y CIENCIAS DE LA SALUD Ingeniería tisular regeneración ósea Andamio Tissue engineering Bone regeneration Scaffolds