Screening of xylose utilizing and high lipid producing yeast strains as a potential candidate for industrial application

Linnea Qvirist RICARDO VAZQUEZ JUAREZ Thomas Andlid (2022, [Artículo])

"Background: Sustainable production of oil for food, feed, fuels and other lipid-based chemicals is essential to meet the demand of the increasing human population. Consequently, novel and sustainable resources such as lignocel- lulosic hydrolysates and processes involving these must be explored. In this paper we screened for naturally-occurring xylose utilizing oleaginous yeasts as cell factories for lipid production, since pentose sugar catabolism plays a major role in efcient utilization of lignocellulosic feedstocks. Glycerol utilization, which is also benefcial in yeast-based oil production as glycerol is a common by-product of biodiesel production, was investigated as well. Natural yeast isolates were studied for lipid accumulation on a variety of substrates, and the highest lipid accumulating strains were further investigated in shake fask cultivations and fermenter studies on xylose and hydrolysate. Results: By collecting leaves from exotic plants in greenhouses and selective cultivation on xylose, a high frequency of oleaginous yeasts was obtained (>40%). Diferent cultivation conditions lead to diferences in fatty acid contents and compositions, resulting in a set of strains that can be used to select candidate production strains for diferent purposes. In this study, the most prominent strains were identifed as Pseudozyma hubeiensis BOT-O and Rhodosporidium toruloides BOT-A2. The fatty acid levels per cell dry weight after cultivation in a nitrogen limited medium with either glucose, xylose or glycerol as carbon source, respectively, were 46.8, 43.2 and 38.9% for P. hubeiensis BOT-O, and 40.4, 27.3 and 42.1% for BOT-A2. Furthermore, BOT-A2 accumulated 45.1% fatty acids per cell dry weight in a natural plant hydrolysate, and P. hubeiensis BOT-O showed simultaneous glucose and xylose consumption with similar growth rates on both carbon sources. The fatty acid analysis demonstrated both long chain and poly-unsaturated fatty acids, depending on strain and medium. Conclusions: We found various natural yeast isolates with high lipid production capabilities and the ability to grow not only on glucose, but also xylose, glycerol and natural plant hydrolysate. R. toruloides BOT-A2 and P. hubeiensis BOT-O specifcally showed great potential as production strains with high levels of storage lipids and comparable growth to that on glucose on various other substrates, especially compared to currently used lipid production strains..."

Microbial lipids, Oleaginous yeast, Lignocellulose, Pseudozyma hubeiensis, Rhodosporidium toruloides BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA MICROBIOLOGÍA MICROBIOLOGÍA MICROBIOLOGÍA

Indicadores de estrés y desempeño biológico de juveniles de lobina rayada (Morone saxatilis) en agua marina: efecto de la densidad de cultivo y color del tanque

Stress indicators and biological performance of striped bass (Morone saxatilis) juvenile in seawater: effect stock density and tank color

DENISE ENCARNACION MAYORAL NORIEGA (2023, [Tesis de maestría])

Existen factores ambientales de naturaleza física, química o biológica que afectan directamente la alimentación de los peces. Por ejemplo, las altas densidades de cultivo pueden generar estrés en los organismos, resultando en la merma del consumo de alimento y por ende su crecimiento. Sin embargo, algunas especies toleran altas densidades formando un grupo o cardumen que les confiere un mejor desempeño. Otro factor que afecta el comportamiento de algunas especies es la coloración de los tanques de cultivo; demostrando la necesidad de utilizar tanques con un entorno físico adecuado para la especie a cultivar. Actualmente, M. saxatilis se produce en tanques en laboratorio y se engorda en jaulas marinas en Baja California; así que existe un gran interés por mejorar su crecimiento y eficiencia alimenticia. Por ello, el objetivo de esta investigación fue evaluar tres densidades de cultivo (alta: 6.2 kg·m-3, media: 3.1 kg·m-3 y baja: 1.5 kg·m-3) y dos colores de tanque (azul celeste y negro) en el crecimiento, supervivencia, utilización del alimento, índices corporales y concentración de cortisol y glucosa de juveniles de lobina rayada en agua marina para su mantenimiento en laboratorio durante nueve semanas. Los resultados demostraron que los organismos obtuvieron el mayor crecimiento en peso en la densidad media, indistintamente del color del tanque. Asimismo, la densidad afectó significativamente los índices hepatosomático y liposomático, ya que disminuyeron conforme aumentó la densidad. El color del tanque influyó de manera importante en el consumo de alimento (azules: 37.4 ± 1.9 g·pez-1; negros: 35.9 ± 2.0 g·pez-1). La interacción de los factores afectó la TCE, TCA y TEP. Las concentraciones de cortisol (81.8 ± 22.5 ng·ml-1) y glucosa (83.2 ± 11.4 mg·100 ml-1) fueron similares en todos los tratamientos y relativamente bajas para la especie. En conclusión, se logró elucidar bajo qué condiciones de cultivo en laboratorio los organismos lograron superar los factores de estrés sin comprometer su crecimiento y supervivencia, reflejándose en un mejor desempeño biológico. Se recomienda utilizar tanques azules o negros con densidades iniciales de 4.2 kg·m-3 o 233 peces·m-3 para el cultivo de juveniles de M. saxatilis en agua salada bajo condiciones de laboratorio.

Environmental factors of a physical, chemical or biological nature such as high stocking densities can directly affect fish feeding and generate stress in organisms, resulting in a decrease in food intake and therefore growth. However, some species tolerate high densities, forming a group or shoal resulting in better overall performance. Another factor that influences the behavior of some species is the color of the culture tank; warranting the need to use tanks with a physical environment suitable for the species to be cultivated. Currently, striped bass are produced in tank-based hatcheries to be socked in sea cages for growout in Baja California. Thus, there is interest in improving their growth rate and feed efficiency under laboratory conditions. Therefore, the objective of this research was to evaluate the effect of three stocking densities (high: 6.2 kg m-3, medium: 3.1 kg m-3 and low: 1.5 kg m-3) and two tank colors (light blue and black) on the biological performance (growth, survival, food utilization), somatic indices and fish stress using the concentration of cortisol and glucose in blood serum as response variables of juvenile striped bass cultured in seawater for nine weeks. The results showed that the organisms had greater growth in terms of final weight when they were cultured at the medium stocking density, regardless of tank color. Likewise, density had a significant effect on hepatosomatic and liposomatic indices, with a tendency to decrease as density increased. The color of the tank significantly influenced the food consumption (blues: 37.4 ± 1.9 g·fish-1; blacks: 35.9 ± 2.0 g·fish-1). The interaction of the factors influenced TCE, TCA and TEP. The concentrations of cortisol (81.8 ± 22.5 ng·ml-1) and glucose (83.2 ± 11.4 mg·100 ml-1) were similar in all treatments indicating low fish stress. In conclusion, it was possible to elucidate under which laboratory culture conditions the organisms were able to overcome the stress factors without compromising their growth and survival; reflected in better biological performance. A stocking density of 4.2 kg m-3 o 233 fish·m-3 for M. saxatilis juvenile reared in saltwater under laboratory culture conditions is recommended.

lobina rayada, densidad cultivo, color tanque, estrés, crecimiento striped bass, stocking density, tank color, stress, growth CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA CIENCIAS AGRARIAS PECES Y FAUNA SILVESTRE PISCICULTURA PISCICULTURA

High quality diet improves lipid metabolic profile and breeding performance in the blue-footed booby, a long-lived seabird

ERICK GONZALEZ MEDINA (2018, [Artículo])

Understanding the role of diet in the physiological condition of adults during reproduction and hence its effect on reproductive performance is fundamental to understand reproductive strategies in long-lived animals. In birds, little is known about the influence of the quality of food consumed at the beginning of the reproductive period and its short-term effects on reproductive performance. To assess the role of diet in the physiological condition of female blue-footed booby, Sula nebouxii (BFBO), during reproduction we evaluated whether individual differences in diet (assessed by using δ13C and δ15N values of whole blood from female birds and muscle tissue of the principal prey species) prior to egg laying and during incubation influenced their lipid metabolic profile (measured as triglyceride levels and C:N ratio) and their reproductive performance (defined by laying date, clutch size and hatching success). Females with higher δ15N values in their blood during the courtship and incubation periods had a higher lipid metabolic profile, earlier laying date, greater clutch size (2–3 eggs) and higher hatching success. Females that laid earlier and more eggs (2–3 eggs) consumed more Pacific anchoveta (Cetengraulis mysticetus) and Pacific thread herring (Opisthonema libertate) than did other females. These two prey species also had high amounts of lipids (C:N ratio) and caloric content (Kcal/g fresh weight). The quality of food consumed by females at the beginning of reproduction affected their physiological condition, as well as their short-term reproductive performance. Our work emphasizes the importance of determining the influence of food quality during reproduction to understand the reproductive decisions and consequences in long-lived animals. © 2018 González-Medina et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

nitrogen 15, triacylglycerol, lipid, animal experiment, Article, breeding, carbon nitrogen ratio, clutch size, controlled study, courtship, diet, egg laying, female, food intake, hatching, lipid metabolism, muscle tissue, nonhuman, prey, reproduction CIENCIAS FÍSICO MATEMÁTICAS Y CIENCIAS DE LA TIERRA CIENCIAS DE LA TIERRA Y DEL ESPACIO OCEANOGRAFÍA OCEANOGRAFÍA