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Molecular Cloning of a cDNA Encoding for Taenia solium TATA-Box Binding Protein 1 (TsTBP1) and Study of Its Interactions with the TATA-Box of Actin 5 and Typical 2-Cys Peroxiredoxin Genes.

Oscar Rodriguez Lima Ponciano Garcia Gutierrez Lucía Jiménez Angel Alfonso Zarain Herzberg Roberto Carlos Lazzarini Lechuga Abraham Landa Piedra (2015)

TATA-box binding protein (TBP) is an essential regulatory transcription factor for the TATA-box and TATA-box-less gene promoters. We report the cloning and characterization of a full-length cDNA that encodes a Taenia solium TATA-box binding protein 1 (TsTBP1). Deduced amino acid composition from its nucleotide sequence revealed that encodes a protein of 238 residues with a predicted molecular weight of 26.7 kDa, and a theoretical pI of 10.6. The NH2-terminal domain shows no conservation when compared with to pig and human TBP1s. However, it shows high conservation in size and amino acid identity with taeniids TBP1s. In contrast, the TsTBP1 COOH-terminal domain is highly conserved among organisms, and contains the amino acids involved in interactions with the TATA-box, as well as with TFIIA and TFIIB. In silico TsTBP1 modeling reveals that the COOH-terminal domain forms the classical saddle structure of the TBP family, with one ?-helix at the end, not present in pig and human. Native TsTBP1 was detected in T. solium cysticerci´s nuclear extract by western blot using rabbit antibodies generated against two synthetic peptides located in the NH2 and COOH-terminal domains of TsTBP1. These antibodies, through immunofluorescence technique, identified the TBP1 in the nucleus of cells that form the bladder wall of cysticerci of Taenia crassiceps, an organism close related to T. solium. Electrophoretic mobility shift assays using nuclear extracts from T. solium cysticerci and antibodies against the NH2-terminal domain of TsTBP1 showed the interaction of native TsTBP1 with the TATA-box present in T. solium actin 5 (pAT5) and 2-Cys peroxiredoxin (Ts2-CysPrx) gene promoters; in contrast, when antibodies against the anti-COOH-terminal domain of TsTBP1 were used, they inhibited the binding of TsTBP1 to the TATA-box of the pAT5 promoter gene.

Article

Parasitología Taenia solium Factores de transcripción Genómica Producción de anticuerpos Proteomica BIOLOGÍA Y QUÍMICA

Genómica Funcional de Plantas: Estudio del Desarrollo de Flores y Frutos

NAYELLI MARSCH MARTINEZ VICTOR MANUEL ZUÑIGA MAYO JOSE IREPAN REYES OLALDE STEFAN DE FOLTER (2012)

La última fase del desarrollo floral es la fertilización de los óvulos y la formación de los frutos, que son muy importantes tanto biológica como económicamente. Notoriamente, más del 80% de los alimentos que son consumidos por el ser humano proviene de flores y frutos. La obtención de conocimientos acerca de las bases moleculares del desarrollo de frutos en especies modelo es de gran interés científico, y un paso indispensable para poder facilitar investigaciones y de ser factible, aplicaciones en frutos de consumo humano. Especialmente en un país como México, con tal riqueza en la diversidad de frutos, este tipo de estudios es necesario y científicamente muy interesante, y tiene repercusiones económicas potenciales importantes. La meta del laboratorio es descubrir genes nuevos involucrados en el desarrollo de frutos, empleando los recursos que brindan plantas modelo como Arabidopsis thaliana. Se hace un enfoque especial en genes que afectan la identidad celular, morfología y que causan partenocarpia (frutos carentes de semillas), para más tarde estudiarlos en otras especies y hacer ensayos para conocer sus alcances en dichas especies.

The last stages of fl oral development are ovule fertilization and fruit formation. Fruits are very important both biologically and economically. Notably, more than 80% of human food is obtained from fl owers and fruits. Gathering basic knowledge about the molecular mechanisms of fruit development from model species is of great scientific interest, and is an essential step to facilitate research and, when feasible, applications in fruits consumed by humans. Especially in countries like Mexico, which has such a great diversity of fruits, this kind of research is both necessary and scientifi cally interesting, and has potentially important economic repercussions. The goal of the lab is to discover new genes that are involved in flower development, making use of the resources provided by model plants like Arabidopsis thaliana. A special focus is made on genes and processes that can affect cell and tissue identity, morphology, and that can cause parthenocarpy (fruits without seed). These genes and processes can then be studied in other species and their effects in those species assessed.

Article

CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA Factores de Transcripción Desarrollo de flores y frutos Genómica Arabidopsis Transcription factors Flower and fruit development Genomics

La genómica en la investigación científica y en la gestión de la vida silvestre en México

The use of Genomics in scientific research and management of wildlife in Mexico

JULIO CESAR CANALES DELGADILLO LEONARDO CHAPA VARGAS MAURICIO COTERA CORREA LAURA MAGDALENA SCOTT MORALES (2015)

"México alberga una diversidad biológica excepcional que lo coloca entre los principales países megadiversos, pues posee tres de las 34 ecorregiones del mundo y zonas consideradas áreas silvestres a nivel mundial, como los desiertos de Chihuahua, Sonora y California; su importancia radica en que reúne alrededor de 70 % de su hábitat original en buenas condiciones y una densidad poblacional humana menor a 5 habitantes km-2. El uso de la genómica como herramienta en la investigación científica en este país tuvo sus inicios a finales de 1930 con trabajos encaminados al mejoramiento genético de cultivos comerciales y a entender los fundamentos ecológicos de la variación genética en Drosophila pseudooscura, pero hasta los años 80 y 90 comenzó el estudio de la flora y la fauna bajo esa perspectiva. Sin embargo, a pesar del potencial que las técnicas genómicas ofrecen para mejorar el desarrollo de estrategias y políticas de gestión que aseguren la producción de alimentos y la preservación de especies, no han sido extensamente utilizadas. Se presenta una revisión de las áreas del conocimiento en la vida silvestre en las que la genómica ha sido incorporada para abordar poblaciones naturales y se discuten los aspectos en los que puede incidir dentro del manejo y conservación de taxa de importancia biológica y comercial."

"Mexico has a unique biodiversity that places it within the list of megadiverse countries; it has three of the 34 ecoregions of the world and sites that are considered wilderness areas worldwide. The use of Genomics as a tool for research in Mexico began in the late 1930s with work aimed at the genetic improvement of commercial crops and to understand the ecological foundations of the genetic variation in Drosohpila pseudooscura, however, it wasn't until the decades of 1980-1990s that these tools were used for the study of natural populations of flora and fauna with purposes of conservation and management. Nonetheless, the potential that genomic tools have to improve the strategies and policies of management to ensure food production and conservation of wildlife in Mexico, these have not been widely applied. In this paper the areas of knowledge in wildlife where genomics have been applied in the study of natural populations of flora and fauna in Mexico were reviewed, and the practical applications of genomics for management and conservation of species of biological and commercial concern were discussed."

Article

ADN Conservación Genómica Manejo Marcadores moleculares Vida silvestre BIOLOGÍA Y QUÍMICA