Advanced search


Knowledge area




436 results, page 1 of 10

Overexpression of AtGRDP2, a novel glycine-rich domain protein, accelerates plant growth and improves stress tolerance

MARIA AZUCENA ORTEGA AMARO AIDA ARACELI RODRIGUEZ HERNANDEZ MARGARITA RODRIGUEZ Y DOMINGUEZ KESSLER ELOISA HERNANDEZ LUCERO SERGIO ROSALES MENDOZA ALEJANDRO IBAÑEZ SALAZAR PABLO DELGADO SANCHEZ JUAN FRANCISCO JIMENEZ BREMONT (2015)

"Proteins with glycine-rich signatures have been reported in a wide variety of organisms including plants, mammalians, fungi, and bacteria. Plant glycine-rich protein genes exhibit developmental and tissue-specific expression patterns. Herein, we present the characterization of the AtGRDP2 gene using Arabidopsis null and knockdown mutants and, Arabidopsis and lettuce over-expression lines. AtGRDP2 encodes a short glycine-rich domain protein, containing a DUF1399 domain and a putative RNA recognition motif (RRM). AtGRDP2 transcript is mainly expressed in Arabidopsis floral organs, and its deregulation in Arabidopsis Atgrdp2 mutants and 35S::AtGRDP2 over-expression lines produces alterations in development. The 35S::AtGRDP2 over-expression lines grow faster than the WT, while the Atgrdp2 mutants have a delay in growth and development. The over-expression lines accumulate higher levels of indole-3-acetic acid and, have alterations in the expression pattern of ARF6, ARF8, and miR167 regulators of floral development and auxin signaling. Under salt stress conditions, 35S::AtGRDP2 over-expression lines displayed higher tolerance and increased expression of stress marker genes. Likewise, transgenic lettuce plants over-expressing the AtGRDP2 gene manifest increased growth rate and early flowering time. Our data reveal an important role for AtGRDP2 in Arabidopsis development and stress response, and suggest a connection between AtGRDP2 and auxin signaling."

Article

Glycine-rich domain protein Arabidopsis thaliana Lactuca sativa Development Indole-3-acetic acid Salt stress BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA

Differences behind the appearances: Export Growth, technological capabilities, and development in Mexico and South Korea

RAUL DELGADO WISE Noela Invernizzi (2005)

In spite of the apparent parallel experiences followed by export growth in Mexico and South Korea, the authors argue that the two countries have followed radically contrasting economic and social development paths. The aim of this paper is to examine some of the strategic dimensions of the two paths in an attempt to demystify the aura of success created around the Mexican case.

La croissance des exportations a suivi des voies apparemment parallèles au Mexique et en Corée du Sud. Les auteurs soutiennent toutefois que le développement économique et social y a emprunté des voies radicalement différentes et opposées. Le but de leur article est d’examiner certaines dimensions stratégiques de ces deux voies pour tenter de démystifier l’aura de succès qui entoure le cas mexicain.

Producción Científica de la Universidad Autónoma de Zacatecas UAZ

Article

CIENCIAS SOCIALES Export Growth Technological Capabilities Development Exportaciones Capacidades tecnológicas Desarrollo

Gonadectomy and progesterone treatment induce protection in murine cysticercosis.

JOSE ANTONIO VARGAS VILLAVICENCIO CARLOS LARRALDE RANGEL JORGE MORALES MONTOR (2006)

The effects of progesterone on castrated mice of both sexes infected with Taenia crassiceps cysticerci were studied. Gonadectomy and treatment with progesterone before infection decreased parasite loads by 100% compared with intact uninfected mice. mRNA levels of IFN gamma and IL-2 (typically associated to Th1-like profiles) were markedly decreased in infected gonadectomized (Gx) mice, whereas progesterone treatment of infected Gx mice did not affected its expression. mRNA levels of IL-4, and IL-10 (typically associated with Th2-like profiles) were reduced by gonadectomy, whereas restitution with progesterone did not affected this pattern in infected Gx progesterone-treated mice. Infection markedly induced expression of progesterone receptor isoform A in splenocytes of Gx mice (5-fold), whereas isoform B had no changes.

Progesterone metabolism to dehydroepiandrosterone (DHEA) in Gx animals was increased 3-fold only in infected progesterone-treated uninfecteds of both sexes, but was not detectable in infected Gx progesterone-treated mice. Conversely, DHEA levels increased 100-fold in infected Gx progesterone-treated mice. However, androgen receptor expression in splenocytes of male mice showed a reduction by gonadectomy, and by infection, whereas in females AR expression showed no changes in the different mouse groups. These results suggest that progesterone, through its metabolism to DHEA, negatively affects the establishment, growth, and reproduction of Taenia crassiceps, by a mechanism that does not implicate a classic genomic pathway involving a nuclear androgen receptor.

Article

MEDICINA Y CIENCIAS DE LA SALUD Adyuvantes inmunológicos - Metabolismo Adyuvantes inmunológicos - Farmacologia Cisticercosis - Parasitología Cisticercosis - Efectos de drogas Deshidroepiandrosterona - Metabolismo Deshidroepiandrosterona - Farmacología Ratones consanguíneos BALB C - Ratones Orquiectomía Progesterona - Administración & dosificación Progesterona - Metabolismo Progesterona - Uso terapéutico Taenia - Efectos de drogas Taenia - Crecimiento & desarrollo Resultado del tratamiento Adjuvants, immunologic - Metabolism Adjuvants, immunologic - Pharmacology Cysticercosis - Immunology Cysticercosis - Parasitology Cysticercus - Drug effects Dehydroepiandrosterone- Metabolism Dehydroepiandrosterone - Pharmacology Mice, inbred BALB C Orchiectomy Ovariectomy Progesterone - Administration & dosage Progesterone - Metabolism Progesterone - Therapeutic use Progestins - Administration & dosage Progestins - Metabolism Progestins - Therapeutic use Taenia - Drug effects Taenia - Growth & development Treatment outcome Cisticercosis Inmunoendocrinos Metabolismo Progesterona Cysticercosis Immunoendocrine Progesterone Taenia

Treatment with dehydroepiandrosterone in vivo and in vitro inhibits reproduction, growth and viability of Taenia crassiceps metacestodes.

JOSE ANTONIO VARGAS VILLAVICENCIO CARLOS LARRALDE RANGEL JORGE MORALES MONTOR (2008)

El objetivo de este trabajo fue explorar el efecto de la deshidroepiandrosterona (DHEA) sobre el establecimiento, crecimiento y reproducción del estadio metacestode de la tenia Taenia crassiceps, tanto in vivo como in vitro. La administración de DHEA antes de la infección en ratones de ambos sexos redujo la carga parasitaria en un 50% en comparación con ratones no tratados. Este efecto protector no se asoció con la respuesta inmune, ya que no hubo efecto del tratamiento con DHEA en los niveles de ARNm de IL-2, IFN-gamma, IL-4 o IL-10. El tratamiento con DHEA de ratones infectados aumentó la expresión del receptor de andrógenos en esplenocitos de ambos sexos. Además, el tratamiento in vitro de T. crassiceps con DHEA redujo la reproducción, la motilidad y la viabilidad de una manera dependiente de la dosis y del tiempo. Los resultados indican que la DHEA tiene fuertes efectos negativos moduladores directos sobre la cisticercosis murina. Sugerimos el uso de análogos hormonales con fines de protección como un enfoque terapéutico para prevenir la cisticercosis murina.

The aim of this work was to explore the effect of dehydroepiandrosterone (DHEA) on the establishment,  growth  and reproduction  of the metacestode stage of the tapeworm Taenia crassiceps, both in vivo and in vitro. Administration of DHEA prior to infection in mice of both sexes reduced the parasite load by 50% compared with untreated mice. This protective effect was not associated with the immune response, since there was no effect of DHEA treatment on mRNA levels of IL-2, IFN-gamma, IL-4 or IL-10. DHEA treatment of infected mice increased androgen receptor expression in splenocytes of both sexes. Moreover, in vitro treatment of T. crassiceps with DHEA reduced reproduction, motility and viability in a dose- and time-dependent fashion. Results indicate that DHEA has strong negative direct modulatory effects on murine cysticercosis. We suggest the use of hormonal-analogues for protective purposes as a therapeutic approach to prevent murine cysticercosis.

Article

MEDICINA Y CIENCIAS DE LA SALUD Adyuvantes inmunológicos - Sangre Adyuvantes inmunológicos - Farmacología Adyuvantes inmunológicos - Uso terapeutico Cisticercosis - Quimioterapia Cisticercosis - Inmunología Cisticercosis - Parasitología Cisticercosis - Crecimiento y desarrollo Cisticercosis - Fisiología Deshidroepiandrosterona - Sangre Deshidroepiandrosterona - Farmacología Eshidroepiandrosterona - Uso terapeutico Ratones consanguíneos BALB C Taenia - Efecto de drogas Taenia -Fisiología Adjuvants, immunologic - Blood Adjuvants, immunologic - Pharmacology Adjuvants, immunologic - Therapeutic use Cysticercosis - Drug therapy Cysticercosis - Immunology Cysticercosis - Parasitology Cysticercus - Growth & development Cysticercus - Physiology Dehydroepiandrosterone - Blood Dehydroepiandrosterone - Pharmacology Dehydroepiandrosterone - Therapeutic use Mice, inbred BALB C Taenia - Drug effects Taenia - Physiology DHEA Tenia Parásito Taenia crassiceps Parasite Infection Hormones Cysticercosis

CRECIMIENTO ALOMÉTRICO EN LARVAS MANJUARÍ (Atractosteus tristoechus)

Andres Hurtado (2013)

Se evaluó el crecimiento alométrico del manjuarí (Atractosteus tristoechus) en larvas mantenidas a una temperatura constante (28 mas menos 1 oC) desde la eclosión hasta los 18 días despues de eclosionadas (DDE).

Article

CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA Zeamays L. Fish larvae allometry development growth

Tracking Nanotechnology in México

EDGAR ZAYAGO LAU Guillermo Foladori (2007)

In Latin America, Brazil, Argentina and México are the leading countries in nanotechnology research and development. However, México is the only one from this group without a national plan concerning the development of nanotechnology and the nanosciences. Regardless, México has signed several multilateral agreements between research centers, foreign universities and industries from overseas to promote the development of the diminutive science. For the most part, there are two features that distinguish the Argentinean and Brazilian initiatives from the Mexican. On one hand, México is missing a tangible plan for the development and research of nanotechnology. On the other, the United States plays an important role in most of the cooperation agreements signed by México and in the creation of new positions inside Mexican high technology industries. This article examines the paths that México has taken in the development of nanotechnology.

Producción Científica de la Universidad Autónoma de Zacatecas UAZ

Article

CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA Nanotechnology México Development Research

El maguey pulquero y su aprovechamiento en el ejido de San José de Los Molinos, municipio de Perote, Veracruz.

ALBERTO ULISES NARVÁEZ SUÁREZ (2015)

Tesis (Maestría en Ciencias, especialista en Desarrollo Rural).- Colegio de Postgraduados, 2015.

La investigación busca determinar las causas que han llevado al borde de la extinción a la planta de maguey pulquero en el ejido de San José de Los Molinos, municipio de Perote, Veracruz. Se analiza si el aprovechamiento integral del maguey es una opción viable que puede contribuir al desarrollo del ejido; asimismo señalar las causas que llevan a reducir su cultivo y determinar cuáles son los problemas que enfrentan los campesinos en la producción de maguey y sus derivados. La metodología del estudio es de tipo mixto: la recolección de información cualitativa se obtiene a través de observación participativa, entrevistas y testimonios así como el método etnográfico con la participación de productores y personajes clave tanto de la comunidad como del municipio. La recolección de datos cuantitativos a través de la aplicación de una encuesta con preguntas cerradas a los ejidatarios de la comunidad. Los resultados obtenidos muestran que la planta de maguey pulquero está en peligro de extinción debido a la extracción de tepezil, un mineral utilizado como insumo para la industria de la construcción; y por la escasa siembra y cuidado de la planta. Estas causan han traído la degradación de la mayoría de las tierras cultivables del ejido y no ha contribuido a resolver los problemas económicos de la población. Se concluye, el aprovechamiento del maguey pulquero es rentable y una opción factible para el desarrollo del ejido si se cultiva, aprovechando la técnica adecuada, representa una alternativa viable en el aspecto económico, ecológico y social. _______________ MAGUEY PULQUERO AND ITS USE IN THE EJIDO OF SAN JOSE DE LOS MOLINOS, MUNICIPALITY OF PEROTE, VERACRUZ. ABSTRACT: The investigation seeks to determine the causes that led to the extinction of pulquero maguey plant in the ejido of San Jose de Los Molinos, municipality of Perote, Veracruz. It examines whether the integral use of the maguey is a viable option that can help develop the ejido; also identify the causes that lead to reducing cultivation and identify problems faced by farmers in the production of maguey and its derivatives are. The study methodology is of mixed type: the collection of qualitative information obtained through participant observation, interviews and testimonies as well as the ethnographic method involving producers and key players from both the community and the municipality. The collection of quantitative data through the application of a survey with closed questions ejidatarios community. The results show that pulquero maguey plant is endangered because tepezil extraction, a mineral used as input for the construction industry; and poor planting and plant care. These have brought cause degradation of most of the arable land of the ejido and has not helped to solve the economic problems of the population. In conclusion, the use of maguey pulquero is cost effective and feasible option for the development of ejido if grown, using the proper technique represents a viable alternative in the economic, ecological and social aspects.

Master thesis

Maguey pulquero Degradación Desarrollo Sustentabilidad Pulquero maguey Degradation Development Sustainability Desarrollo Rural Maestría INGENIERÍA Y TECNOLOGÍA CIENCIAS TECNOLÓGICAS TECNOLOGÍA DE LOS ALIMENTOS BIOQUÍMICA Y MICROBIOLOGÍA DE LOS PROCESOS FERMENTATIVOS

Simultaneous silencing of two arginine decarboxylase genes alters development in Arabidopsis

DIANA SANCHEZ RANGEL ANA ISABEL CHAVEZ MARTINEZ AIDA ARACELI RODRIGUEZ HERNANDEZ ISRAEL MARURI LOPEZ Kazuo Shinozaki JUAN FRANCISCO JIMENEZ BREMONT (2016)

"Polyamines (PAs) are small aliphatic polycations that are found ubiquitously in all organisms. In plants, PAs are involved in diverse biological processes such as growth, development, and stress responses. In Arabidopsis thaliana, the arginine decarboxylase enzymes (ADC1 and 2) catalyze the first step of PA biosynthesis. For a better understanding of PA biological functions, mutants in PA biosynthesis have been generated; however, the double adc1/adc2 mutant is not viable in A. thaliana. In this study, we generated non-lethal A. thaliana lines through an artificial microRNA that simultaneously silenced the two ADC genes (amiR:ADC). The generated transgenic lines (amiRADC-L1 and -L2) showed reduced AtADC1 and AtADC2 transcript levels. For further analyses the amiR:ADC-L2 line was selected. We found that the amiR:ADC-L2 line showed a significant decrease of their PA levels. The co-silencing revealed a stunted growth in A. thaliana seedlings, plantlets and delay in its flowering rate; these phenotypes were reverted with PA treatment. In addition, amiR:ADC-L2 plants displayed two seed phenotypes, such as yellow and brownish seeds. The yellow mutant seeds were smaller than adc1, adc2 mutants and wild type seeds; however, the brownish were the smallest seeds with arrested embryos at the torpedo stage. These data reinforce the importance of PA homeostasis in the plant development processes."

Article

Artificial microRNA Arabidopsis thaliana Arginine decarboxylase Polyamines Development Seed BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA BIOLOGÍA MOLECULAR