Genetic analysis of Vibrio parahaemolyticus O3:K6 strains that have been isolated in Mexico since 1998

CARLOS ABRAHAM GUERRERO RUIZ (2017, [Artículo])

Vibrio parahaemolyticus is an important human pathogen that has been isolated worldwide from clinical cases, most of which have been associated with seafood consumption. Environmental and clinical toxigenic strains of V. parahaemolyticus that were isolated in Mexico from 1998 to 2012, including those from the only outbreak that has been reported in this country, were characterized genetically to assess the presence of the O3:K6 pandemic clone, and their genetic relationship to strains that are related to the pandemic clonal complex (CC3). Pathogenic tdh+ and tdh+/trh+ strains were analyzed by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). Also, the entire genome of a Mexican O3:K6 strain was sequenced. Most of the strains were tdh/ORF8-positive and corresponded to the O3:K6 serotype. By PFGE and MLST, there was very close genetic relationship between ORF8/O3:K6 strains, and very high genetic diversities from non-pandemic strains. The genetic relationship is very close among O3:K6 strains that were isolated in Mexico and sequences that were available for strains in the CC3, based on the PubMLST database. The whole-genome sequence of CICESE-170 strain had high similarity with that of the reference RIMD 2210633 strain, and harbored 7 pathogenicity islands, including the 4 that denote O3:K6 pandemic strains. These results indicate that pandemic strains that have been isolated in Mexico show very close genetic relationship among them and with those isolated worldwide. © 2017 Guerrero et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Article, bacterial strain, biofouling, controlled study, Crassostrea, food intake, gene sequence, genetic analysis, genetic variability, Japan, Mexican, Mexico, molecular phylogeny, nonhuman, pandemic, pathogenicity island, sea food, serotyping, toxi BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA GENÉTICA GENÉTICA

Contrasting spatial patterns in active-fire and fire-suppressed mediterranean climate old-growth mixed conifer forests

Danny L. Fry  (2014, [Artículo])

In Mediterranean environments in western North America, historic fire regimes in frequent-fire conifer forests are highly variable both temporally and spatially. This complexity influenced forest structure and spatial patterns, but some of this diversity has been lost due to anthropogenic disruption of ecosystem processes, including fire. Information from reference forest sites can help management efforts to restore forests conditions that may be more resilient to future changes in disturbance regimes and climate. In this study, we characterize tree spatial patterns using four-ha stem maps from four old-growth, Jeffrey pine-mixed conifer forests, two with active-fire regimes in northwestern Mexico and two that experienced fire exclusion in the southern Sierra Nevada. Most of the trees were in patches, averaging six to 11 trees per patch at 0.007 to 0.014 ha-1, and occupied 27-46% of the study areas. Average canopy gap sizes (0.04 ha) covering 11-20% of the area were not significantly different among sites. The putative main effects of fire exclusion were higher densities of single trees in smaller size classes, larger proportion of trees (≥56%) in large patches (≥10 trees), and decreases in spatial complexity. While a homogenization of forest structure has been a typical result from fire exclusion, some similarities in patch, single tree, and gap attributes were maintained at these sites. These within-stand descriptions provide spatially relevant benchmarks from which to manage for structural heterogeneity in frequent-fire forest types.

article, climate, controlled study, ecosystem fire history, forest structure, geographic distribution, geographic mapping, land use, mathematical computing, mathematical model, Mexico, spatial analysis, taiga, United States, comparative study, conife CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA

The Pacific harbor seal gut microbiota in Mexico: Its relationship with diet and functional inferences

ARLETTE MARIMAR PACHECO SANDOVAL (2019, [Artículo])

Diet is a primary driver of the composition of gut microbiota and is considered one of the main routes of microbial colonization. Prey identification is fundamental for correlating the diet with the presence of particular microbial groups. The present study examined how diet influenced the composition and function of the gut microbiota of the Pacific harbor seal (Phoca vitulina richardii) in order to better understand the role of prey consumption in shaping its microbiota. This species is a good indicator of the quality of the local environment due to both its foraging and haul-out site fidelity. DNA was extracted from 20 fecal samples collected from five harbor seal colonies located in Baja California, Mexico. The V4 region of 16S rRNA gene was amplified and sequenced using the Illumina technology. Results showed that the gut microbiota of the harbor seals was dominated by the phyla Firmicutes (37%), Bacteroidetes (26%) and Fusobacteria (26%) and revealed significant differences in its composition among the colonies. Funtional analysis using the PICRUSt software suggests a high number of pathways involved in the basal metabolism, such as those for carbohydrates (22%) and amino acids (20%), and those related to the degradation of persistent environmental pollutants. In addition, a DNA metabarcoding analysis of the same samples, via the amplification and sequencing of the mtRNA 16S and rRNA 18S genes, was used to identify the prey consumed by harbor seals revealing the consumption of prey with mainly demersal habits. Functional redundancy in the seal gut microbiota was observed, irrespective of diet or location. Our results indicate that the frequency of occurrence of specific prey in the harbor seal diet plays an important role in shaping the composition of the gut microbiota of harbor seals by influencing the relative abundance of specific groups of gut microorganisms. A significant relationship was found among diet, gut microbiota composition and OTUs assigned to a particular metabolic pathway. © 2019 Pacheco-Sandoval et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

RNA 16S, RNA 18S, amino acid analysis, animal food, Article, bacterium colony, Bacteroidetes, basal metabolic rate, biodegradation, controlled study, DNA barcoding, feces analysis, Firmicutes, Fusobacteria, intestine flora, metabolism, Mexico, microb BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA BIOLOGÍA ANIMAL (ZOOLOGÍA) BIOLOGÍA ANIMAL (ZOOLOGÍA)

Maintenance of Coastal Surface Blooms by Surface Temperature Stratification and Wind Drift

MARY CARMEN RUIZ DE LA TORRE (2013, [Artículo])

Algae blooms are an increasingly recurrent phenomenon of potentially socio-economic impact in coastal waters globally and in the coastal upwelling region off northern Baja California, Mexico. In coastal upwelling areas the diurnal wind pattern is directed towards the coast during the day. We regularly found positive Near Surface Temperature Stratification (NSTS), the resulting density stratification is expected to reduce the frictional coupling of the surface layer from deeper waters and allow for its more efficient wind transport. We propose that the net transport of the top layer of approximately 2.7 kilometers per day towards the coast helps maintain surface blooms of slow growing dinoflagellate such as Lingulodinium polyedrum. We measured: near surface stratification with a free-rising CTD profiler, trajectories of drifter buoys with attached thermographs, wind speed and direction, velocity profiles via an Acoustic Doppler Current Profiler, Chlorophyll and cell concentration from water samples and vertical migration using sediment traps. The ADCP and drifter data agree and show noticeable current shear within the first meters of the surface where temperature stratification and high cell densities of L. polyedrum were found during the day. Drifters with 1m depth drogue moved towards the shore, whereas drifters at 3 and 5 m depth showed trajectories parallel or away from shore. A small part of the surface population migrated down to the sea floor during night thus reducing horizontal dispersion. The persistent transport of the surface bloom population towards shore should help maintain the bloom in favorable environmental conditions with high nutrients, but also increasing the potential socioeconomic impact of the blooms. The coast wise transport is not limited to blooms but includes all dissolved and particulate constituents in surface waters. © 2013 Ruiz-de la Torre et al.

chlorophyll, algal bloom, article, cell count, cell density, coastal waters, controlled study, dinoflagellate, Lingulodinium polyedrum, meteorological phenomena, Mexico, near surface temperature stratification, nonhuman, nutrient concentration, popul CIENCIAS FÍSICO MATEMÁTICAS Y CIENCIAS DE LA TIERRA CIENCIAS DE LA TIERRA Y DEL ESPACIO OCEANOGRAFÍA OCEANOGRAFÍA

Aplicación de productos derivados del insecto Dactylopius coccus Costa (Homóptera, Dactylopiidae)

Gabriela Arroyo Figueroa GRACIELA MA. DE LA LUZ RUIZ AGUILAR LORENA VARGAS RODRIGUEZ GUILLERMO GONZALEZ SANCHEZ (2012, [Artículo])

Actualmente muchas industrias buscan sustituir los colorantes sintéticos por naturales, debido a su efecto nocivo y a la alta contaminación del medio ambiente. Del insecto Dactylopius coccus Costa (grana carmín) se obtienen productos naturales que se pueden usar como colorantes, por lo que el objetivo de este trabajo fue difundir la aplicación del insecto grana carmín y los productos derivados del mismo en el teñido de una tela de algodón y en la coloración de una bala labial a nivel laboratorio. Al aplicar el extracto acuoso de la grana carmín sobre la tela de algodón, se consiguieron colores homogéneos, con valores promedio en la escala CIELab de: 43.81±0.33 L*, 44.64±0.15 a* y -4.03±0.55 b*. Asimismo al aplicar el pigmento laca carmín en la elaboración de una bala labial se lograron colores uniformes con los siguientes valores promedio en la escala CIELab: 13.25±3.62 L*, 4.80±1.41 a* y -0.23±0.84 b*.

Nowadays many industries want to replace synthetic dyes by natural, due to its harmful effects and the high contamination of the environment. Dactylopius coccus Costa (cochineal) is an insect to obtain natural products that are used as dyes. The aim of this study

was to promote the application of cochineal and the products derivates of these insect, in the dyeing of cotton fabric and in the coloration of a lipstick bullet at the laboratory. Was applied to the aqueous extract of cochineal on cotton fabric, obtaining homogeneous colors, with average values in the CIELab scale of: 43.81±0.33 L*, 44.64±0.15 a* y -4.03±0.55 b*. It also was applied the carmine pigment in the elaboration of a lipstick bullet, was obtained consistent color with the following average values on the CIELab scale: 13.25±3.62 L*, 4.80±1.41 a* y -0.23±0.84 b*.

CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA Grana Carmín Colorantes naturales Teñido Bala labial Cochineal Natural dye Dyeing Lipstick bullet

Phylogenetic relationships of Pseudo-nitzschia subpacifica (Bacillariophyceae) from the Mexican Pacific, and its production of domoic acid in culture

Sonia Quijano (2020, [Artículo])

Pseudo-nitzschia is a cosmopolitan genus, some species of which can produce domoic acid (DA), a neurotoxin responsible for the Amnesic Shellfish Poisoning (ASP). In this study, we identified P. subpacifica for the first time in Todos Santos Bay and Manzanillo Bay, in the Mexican Pacific using SEM and molecular methods. Isolates from Todos Santos Bay were cultivated under conditions of phosphate sufficiency and deficiency at 16°C and 22°C to evaluate the production of DA. This toxin was detected in the particulate (DAp) and dissolved (DAd) fractions of the cultures during the exponential and stationary phases of growth of the cultures. The highest DA concentration was detected during the exponential phase grown in cells maintained in P-deficient medium at 16°C (1.14 ± 0.08 ng mL-1 DAd and 4.71 ± 1.11 × 10−5 ng cell-1 of DAp). In P-sufficient cultures DA was higher in cells maintained at 16°C (0.25 ± 0.05 ng mL-1 DAd and 9.41 ± 1.23 × 10−7 ng cell-1 of DAp) than in cells cultured at 22°C. Therefore, we confirm that P. subpacifica can produce DA, especially under P-limited conditions that could be associated with extraordinary oceanographic events such as the 2013–2016 "Blob" in the northeastern Pacific Ocean. This event altered local oceanographic conditions and possibly generated the presence of potential harmful species in areas with economic importance on the Mexican Pacific coast. © 2020 Quijano-Scheggia et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

domoic acid, domoic acid, kainic acid, Article, cell growth, controlled study, diatom, Mexico, morphology, nonhuman, Pacific Ocean, phylogeny, plant cell, plant growth, Pseudo nitzschia, toxin analysis, cell culture technique, classification, diatom, CIENCIAS FÍSICO MATEMÁTICAS Y CIENCIAS DE LA TIERRA CIENCIAS DE LA TIERRA Y DEL ESPACIO OCEANOGRAFÍA OCEANOGRAFÍA

Association between nonalcoholic fatty liver disease and severe male reproductive organ impairment (Germinal Epithelial Loss): Study on a mouse model and on human patients

URIEL ALEJANDRO LOPEZ LEMUS RAQUEL GARZA GUAJARDO ORALIA BARBOZA QUINTANA ALEJANDRINA RODRIGUEZ HERNANDEZ José Guzmán Esquivel ALEJANDRO GARCÍA RIVERA VIOLETA M. MADRIGAL PÉREZ LAURA ELVIRA GARCÍA LABASTIDA ALEJANDRO DAVID SORIANO HERNANDEZ MARGARITA DE LA LUZ MARTINEZ FIERRO IRAM PABLO RODRIGUEZ SANCHEZ ELIZABETH SANCHEZ DUARTE ARIANA CABRERA LICONA GABRIEL CEJA ESPIRITU IVAN DELGADO ENCISO (2018, [Artículo])

Metabolic syndrome (MS) has been associated with testicular damage. Nonalcoholic fatty liver disease (NAFLD) is a multisystemic disease that affects different organs, but its effect on the testes is unknown. A study analyzing germ cell involvement on BALB/c mice was carried out. A parallel comparative study was conducted that investigated alterations in the germinal epithelium of male humans that died from an unrelated acute event. The complete medical histories and histologic samples of the thoracic aorta, liver tissue, and testicular tissue from the deceased subjects were collected. The degree of germinal epithelial loss (DGEL) was evaluated and the clinical and histologic data were compared between individuals with and without NAFLD. The only metabolic or morphologic variable that caused a significant difference in the DGEL, in both the animal model and humans, was the presence of liver steatosis. The percentage of steatosis was also correlated with the percentage of the DGEL. In humans, steatosis (greater than 20%) increased the risk 12-fold for presenting with a severe DGEL (OR: 12.5; 95% CI [1.2, 128.9]; p = .03). There was no association with age above 50 years or MS components. Steatosis grade was also correlated with atherosclerosis grade. NAFLD was a strongly associated factor implicated in severe DGEL, as well as the testis was identified as a probable target organ for damage caused by the disease. his finding could result in the search for new approach strategies in the management of men with fertility problems. Further studies are required to confirm these results.

MEDICINA Y CIENCIAS DE LA SALUD Germ cells Atherosclerosis Testis Metabolic syndrome

Thermal and mechanical properties of PLA-based multiscale cellulosic biocomposites

MIGUEL ANGEL RUZ CRUZ Pedro Jesús Herrera Franco Emmanuel Alejandro Flores Johnson MARIA VERONICA MORENO CHULIM LUCIANO MIGUEL GALERA MANZANO Alex Valadez González (2022, [Artículo])

In this work polylactic acid (PLA) based multiscale cellulosic biocomposites were prepared with the aim to evaluate the effect of the incorporation of cellulose nanocrystals (CNCs) on the PLA biocomposites reinforced with cellulose microfibers (MFCs). For this, PLA composite materials reinforced with both MFCs and with a combination of MFCs and CNCs were prepared, while keeping the content of cellulosic reinforcements constant. The thermal and mechanical properties of these multiscale PLA biocomposites were characterized by thermogravimetry (TGA), differential scanning calorimetry (DSC), flexural mechanical and, dynamic mechanical (DMA) tests. Likewise, they were characterized by Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). The results show that the replacement of MFCs by CNCs in the 1–5% range appreciably modifies the thermal and mechanical properties of multiscale compounds. For example, they increase the thermal stability of the materials, modify the PLA crystallization process and play the role of adhesion promoters since the mechanical properties in flexure increase in the order of 40% and the storage modulus increases in the order of 35% at room temperature. Also, the addition of CNCs increases the relaxation temperature of the material from 50 to 60 °C, thereby expanding the temperature range for its use. © 2022 The Author(s)

MULTISCALE BIOCOMPOSITES CELLULOSE MICROFIBER CELLULOSE NANOCRYSTALS HIERARCHICAL STRUCTURE PROPERTIES INGENIERÍA Y TECNOLOGÍA CIENCIAS TECNOLÓGICAS TECNOLOGÍA DE MATERIALES PROPIEDADES DE LOS MATERIALES PROPIEDADES DE LOS MATERIALES

Whole-genome comparison between reference sequences and oyster Vibrio vulnificus C-genotype strains

CARLOS ABRAHAM GUERRERO RUIZ (2019, [Artículo])

Whole-genome sequences of Vibrio vulnificus clinical genotype (C-genotype) from the CICESE Culture Collection, isolated from oysters, were compared with reference sequences of CMCP6 and YJ016 V. vulnificus C-genotype strains of clinical origin. The RAST web server estimated the whole genome to be ~4.8 Mb in CICESE strain 316 and ~4.7 Mb in CICESE strain 325. No plasmids were detected in the CICESE strains. Based on a phylogenetic tree that was constructed with the whole-genome results, we observed high similarity between the reference sequences and oyster C-genotype isolates and a sharp contrast with environmental genotype (E-genotype) reference sequences, indicating that the differences between the C- and E-genotypes do not necessarily correspond to their isolation origin. The CICESE strains share 3488 genes (63.2%) with the YJ016 strain and 3500 genes (63.9%) with the CMCP6 strain. A total of 237 pathogenicity associated genes were selected from reference clinical strains, where—92 genes were from CMCP6, 126 genes from YJ016, and 19 from MO6-24/ O; the presence or absence of these genes was recorded for the CICESE strains. Of the 92 genes that were selected for CMCP6, 67 were present in both CICESE strains, as were as 86 of the 126 YJ016 genes and 13 of the 19 MO6-24/O genes. The detection of elements that are related to virulence in CICESE strains—such as the RTX gene cluster, vvhA and vvpE, the type IV pili cluster, the XII genomic island, and the viuB genes, suggests that environmental isolates with the C-genotype, have significant potential for infection. © 2019 Guerrero et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Article, bacterial gene, bacterial strain, bacterial virulence, comparative study, controlled study, gene cluster, gene identification, genomic island, genotype, nonhuman, phylogenetic tree, sequence analysis, strain identification, Vibrio vulnificus BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA GENÉTICA GENÉTICA

Editorial: Social and institutional innovations for achieving sustainable agricultural transformation in the global south

Paswel Marenya Jeetendra Aryal Annet Mulema Dil Bahadur Rahut (2023, [Artículo])

Agrifood Systems Transformation Global South Institutional Innovations CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA AGRIFOOD SYSTEMS GREEN REVOLUTION INNOVATION SUSTAINABLE DEVELOPMENT