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Genome-wide association analyses of agronomic traits and Striga hermonthica resistance in pearl millet

Hussein Shimelis Chris Ojiewo Abhishek Rathore (2023, [Artículo])

Pearl millet (Pennisetum glaucum [L.] R. Br.) is a nutrient-dense, relatively drought-tolerant cereal crop cultivated in dry regions worldwide. The crop is under-researched, and its grain yield is low (< 0.8 tons ha−1) and stagnant in the major production regions, including Burkina Faso. The low productivity of pearl millet is mainly attributable to a lack of improved varieties, Striga hermonthica [Sh] infestation, downy mildew infection, and recurrent heat and drought stress. Developing high-yielding and Striga-resistant pearl millet varieties that satisfy the farmers’ and market needs requires the identification of yield-promoting genes linked to economic traits to facilitate marker-assisted selection and gene pyramiding. The objective of this study was to undertake genome-wide association analyses of agronomic traits and Sh resistance among 150 pearl millet genotypes to identify genetic markers for marker-assisted breeding and trait introgression. The pearl millet genotypes were phenotyped in Sh hotspot fields and screen house conditions. Twenty-nine million single nucleotide polymorphisms (SNPs) initially generated from 345 pearl millet genotypes were filtered, and 256 K SNPs were selected and used in the present study. Phenotypic data were collected on days to flowering, plant height, number of tillers, panicle length, panicle weight, thousand-grain weight, grain weight, number of emerged Striga and area under the Striga number progress curve (ASNPC). Agronomic and Sh parameters were subjected to combined analysis of variance, while genome-wide association analysis was performed on phenotypic and SNPs data. Significant differences (P < 0.001) were detected among the assessed pearl millet genotypes for Sh parameters and agronomic traits. Further, there were significant genotype by Sh interaction for the number of Sh and ASNPC. Twenty-eight SNPs were significantly associated with a low number of emerged Sh located on chromosomes 1, 2, 3, 4, 6, and 7. Four SNPs were associated with days-to-50%-flowering on chromosomes 3, 5, 6, and 7, while five were associated with panicle length on chromosomes 2, 3, and 4. Seven SNPs were linked to thousand-grain weight on chromosomes 2, 3, and 6. The putative SNP markers associated with a low number of emerged Sh and agronomic traits in the assessed genotypes are valuable genomic resources for accelerated breeding and variety deployment of pearl millet with Sh resistance and farmer- and market-preferred agronomic traits.

High-Yielding Varieties Striga-Resistant Agronomic Parameters CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA GENOME-WIDE ASSOCIATION STUDIES STRIGA HERMONTHICA PEARL MILLET

The potential of UAV and very high-resolution satellite imagery for yellow and stem rust detection and phenotyping in Ethiopia

Gerald Blasch David Hodson Francelino Rodrigues (2023, [Artículo])

Very high (spatial and temporal) resolution satellite (VHRS) and high-resolution unmanned aerial vehicle (UAV) imagery provides the opportunity to develop new crop disease detection methods at early growth stages with utility for early warning systems. The capability of multispectral UAV, SkySat and Pleiades imagery as a high throughput phenotyping (HTP) and rapid disease detection tool for wheat rusts is assessed. In a randomized trial with and without fungicide control, six bread wheat varieties with differing rust resistance were monitored using UAV and VHRS. In total, 18 spectral features served as predictors for stem and yellow rust disease progression and associated yield loss. Several spectral features demonstrated strong predictive power for the detection of combined wheat rust diseases and the estimation of varieties’ response to disease stress and grain yield. Visible spectral (VIS) bands (Green, Red) were more useful at booting, shifting to VIS–NIR (near-infrared) vegetation indices (e.g., NDVI, RVI) at heading. The top-performing spectral features for disease progression and grain yield were the Red band and UAV-derived RVI and NDVI. Our findings provide valuable insight into the upscaling capability of multispectral sensors for disease detection, demonstrating the possibility of upscaling disease detection from plot to regional scales at early growth stages.

Very High Resolution Imagery Disease Detection Methods Early Growth Stages CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA UNMANNED AERIAL VEHICLES STEM RUST PHENOTYPING HIGH-THROUGHPUT PHENOTYPING WHEAT

Ajustar las condiciones de operación de una prensa hidráulica horizontal de fabricación propia para un proceso de doblado en frío a 90°

Hugo Estrada Pimentel HUGO ARCOS GUTIERREZ JOSE ANTONIO BETANCOURT CANTERA Jan Mayén Chaires John Edison García Herrera (2023, [Artículo])

During the cold-bending process, the tooling suffers considerable damage due to excess pressure. This also causes the machines to break down, causing problems in the precision and quality of the metal parts formed. The precision depends on the operating conditions of the press, the tooling employed, and the elastic recovery effect of the material. This study determines the working conditions for a made-in-house horizontal hydraulic press through an experimental design (DOE). This research carried out the V-forming to 90° (ISO 2768-1) of a hot-rolled carbon steel plate, considering pressure, piston permanence time, and recovery factor (Kr). The experimental and statistical analysis ensures accurate forming while the work pressure decreases by 17% and 33%, respectively, regarding the maximum. This reduction will delay the appearance of fatigue damage and have the operating parameters well established; in turn, it will be possible to design tools according to commercial standards.

Durante el proceso de conformado en frío, las herramientas sufren daños considerables debido al exceso de presión. Esto también causa que las máquinas se rompan, causando problemas en la precisión y calidad de las piezas metálicas formadas. La precisión depende de las condiciones de funcionamiento de la prensa, las herramientas empleadas y el efecto elástico de recuperación del material. Este estudio determina las condiciones de trabajo de una prensa hidráulica horizontal fabricado en casa a través de un diseño experimental (DOE). Esta investigación llevó a cabo el conformado en V a 90° (ISO 2768-1) de una placa de acero al carbono laminada en caliente, teniendo en cuenta la presión, el tiempo de permanencia del pistón y el factor de recuperación (Kr). El análisis experimental y estadístico asegura un conformado preciso, mientras que la presión de trabajo disminuyó en un 17% y 33%, respectivamente, con respecto al máximo. Esta reducción retrasará la aparición de daños por fatiga y tendrá los parámetros de funcionamiento bien establecidos; con estos, a su vez, será posible diseñar herramientas de acuerdo con los estándares comerciales.

Authorship acknowledgement: Hugo Estrada: Conceptualization; methodology; validation; writing; revision; project administration; software; investigation; display; draft writing; reviewing and editing. Hugo Arcos: Conceptualization; methodology; validation; writing; revision; simulation; software; reviewing. José A. Betancourt: Supervision; methodology; validation; writing; revision; draft writing: writing reviewing and editing. Jan Mayen: Supervision; methodology; validation; writing; revision; formal analysis.

John E. García: Conceptualization; supervision; methodology; validation; writing; revision; project management; formal analysis, draft writing: writing reviewing and editing.

Cold forming DOE ANOVA Recovery factor (Kr) Factorial analysis 2k Spring back (SB) Conformado en frío Factor de recuperación (Kr) Análisis factorial 2k Recuperación elástica (SB) INGENIERÍA Y TECNOLOGÍA CIENCIAS TECNOLÓGICAS OTRAS ESPECIALIDADES TECNOLÓGICAS OTRAS OTRAS