The banana MaWRKY18, MaWRKY45, MaWRKY60 and MaWRKY70 genes encode functional transcription factors and display differential expression in response to defense phytohormones

SERGIO GARCIA LAYNES VIRGINIA AURORA HERRERA VALENCIA Lilia Guadalupe Tamayo Torres VERONICA LIMONES BRIONES FELIPE ALONSO BARREDO POOL FRAY MARTIN BAAS ESPINOLA Angel Alpuche-Solis CARLOS ALBERTO PUCH HAU SANTY PERAZA ECHEVERRIA (2022, [Artículo])

WRKY transcription factors (TFs) play key roles in plant defense responses through phytohormone signaling pathways. However, their functions in tropical fruit crops, especially in banana, remain largely unknown. Several WRKY genes from the model plants rice (OsWRKY45) and Arabidopsis (AtWRKY18, AtWRKY60, AtWRKY70) have shown to be attractive TFs for engineering disease resistance. In this study, we isolated four banana cDNAs (MaWRKY18, MaWRKY45, MaWRKY60, and MaWRKY70) with homology to these rice and Arabidopsis WRKY genes. The MaWRKY cDNAs were isolated from the wild banana Musa acuminata ssp. malaccensis, which is resistant to several diseases of this crop and is a progenitor of most banana cultivars. The deduced amino acid sequences of the four MaWRKY cDNAs revealed the presence of the conserved WRKY domain of ~60 amino acids and a zinc-finger motif at the N-terminus. Based on the number of WRKY repeats and the structure of the zinc-finger motif, MaWRKY18 and MaWRKY60 belong to group II of WRKY TFs, while MaWRKY45 and MaWRKY70 are members of group III. Their corresponding proteins were located in the nuclei of onion epidermal cells and were shown to be functional TFs in yeast cells. Moreover, expression analyses revealed that the majority of these MaWRKY genes were upregulated by salicylic acid (SA) or methyl jasmonate (MeJA) phytohormones, although the expression levels were relatively higher with MeJA treatment. The fact that most of these banana WRKY genes were upregulated by SA or MeJA, which are involved in systemic acquired resistance (SAR) or induced systemic resistance (ISR), respectively, make them interesting candidates for bioengineering broad-spectrum resistance in this crop. © 2022 by the authors.

BANANA TRANSCRIPTION FACTOR WRKY DEFENSE PHYTOHORMONES SALICYLIC ACID METHYL JASMONATE SAR ISR BROAD-SPECTRUM RESISTANCE BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA GENÉTICA GENÉTICA MOLECULAR DE PLANTAS GENÉTICA MOLECULAR DE PLANTAS

Retención larvaria en abulón azul Haliotis fulgens mediante análisis de parentesco en B.C.S., México

ADRIANA MAX AGUILAR (2014, [Tesis de maestría])

El abulón azul (Haliotis fulgens) es un molusco gasterópodo de importancia comercial en México. Es un recurso de alto valor comercial (70 M en 2011) debido a su gran demanda a nivel internacional; su pesquería constituye una de las actividades más importantes que se desarrollan en la costa occidental de la península de Baja California. Esta pesquería ha sufrido de manera histórica una disminución significativa, por lo que es necesario reforzar y continuar los programas de investigación y manejo actual para garantizar la recuperación de los bancos abuloneros. Un aspecto crucial en el manejo y administración de las pesquerías, es el claro entendimiento del concepto de unidad de stock, el cual requiere un mejor conocimiento del reclutamiento en función de la dinámica larvaria y la conectividad entre bancos (dados por los niveles de retención y dispersión), lo que ampliaría la precisión de las estrategias de manejo actuales. En el caso del abulón se han propuesto dos modelos de conectividad: (1) Las larvas son transportadas a grandes distancias de sus padres y llegan a sustentar otros bancos; y (2) el intercambio larvario entre bancos es limitado y cada banco es un pequeño stock que es autosustentable. En cualquier caso, es necesario determinar si en un banco lo que ocurre preponderantemente es el auto-reclutamiento, o si por el contrario, las larvas provienen principalmente de otros sitios. El objetivo del presente trabajo fue estimar el índice de retención larvaria del abulón azul Haliotis fulgens en dos sitios de la costa occidental de Baja California Sur, mediante el uso de marcadores genéticos tipo microsatélite en análisis de parentesco. Se estandarizaron y caracterizaron nuevos loci microsatélites específicos para H. fulgens con los cuales se determinó el índice de retención larvaria a través de la asignación genética de juveniles a progenitores que se encontraran en el mismo sitio de muestreo mediante análisis de paternidad con el programa CERVUS 3.0. Se obtuvieron 24 loci microsatélites potenciales para utilizarlos en análisis genéticos, de los cuales se caracterizaron 12 loci polimórficos. Se determinó que 11 de ellos son factibles de utilizar en análisis de parentesco por su alto polimorfismo (Número de alelos: de 5-17; HE de 0.69-0.92; PIC de 0.64-0.90) y presentar Equilibrio de Hardy-Weinberg [...]

The green abalone (Haliotis fulgens) is a marine gastropod mollusk in Mexico with a high commercial value (70 M in 2011) because of its high demand in the international market. Its fishery is an important commercial activity in the west coast of Baja California Sur, and has historically suffered a significant decline in its captures. Thus it is necessary to continue research and current management programs to ensure the recovery of abalone in reef systems. A crucial consideration in management and administration of this fishery is a clear understanding of the concept of a stock unit, which requires a better understanding of recruitment on defined spatial scales, depending on the larval dynamics and connectivity among reefs (given by the levels of retention and dispersal). This knowledge will extend the accuracy of current management strategies. Two different models on connectivity have been proposed for abalone: (1) the larvae can be transported for long distances from their parent’s reef and supply other distant reefs; and (2) larval exchange between reefs is limited, and each one is a small self-replenishing bank. In any case, it is necessary to determine whether a reef is mainly maintained by self-recruitment, or if larvae come mainly from other sites. The objective of this work was estimate the proportion of green abalone Haliotis fulgens larval retention in two sites on the western coast of Baja California Sur, using microsatellite markers in paternity analysis. New microsatellite markers in H. fulgens were standardized and characterized. The larval retention was estimated by the proportion of juveniles genetically assigned to the possible parents in the same site by paternity analysis using CERVUS 3.0. Twenty-four microsatellite loci potentially useful in genetic analysis were obtained, from which 12 polymorphic loci were characterized. From these, 11 were useful in paternity analysis due to their high polymorphism (number of alleles: 5-17; HE: 0.69-0.92; PIC: 0.64-0.90) and because they were in Hardy-Weinberg equilibrium [...]

Retención larvaria; microsatélites; auto-reclutamiento BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA GENÉTICA GENÉTICA DE POBLACIONES GENÉTICA DE POBLACIONES

Un gen para ti, un gen para mí: flujo genético entre parientes silvestres y plantas domesticadas

Mauricio Heredia Pech MARIANA CHAVEZ PESQUEIRA (2023, [Artículo])

El flujo genético se refiere al movimiento de genes entre individuos o poblaciones. En ocasiones puede darse también entre especies cercanamente emparentadas, como en el caso de las plantas domesticadas y sus parientes silvestres. Los parientes silvestres son de gran importancia para la seguridad alimentaria debido a su gran diversidad genética, que los convierte en una reserva natural de genes. Las consecuencias del flujo genético silvestre-domesticado dependerán de la intensidad y dirección en la que se muevan los genes, siendo la preocupación principal el riesgo que puede existir para el mantenimiento de la diversidad genética de los parientes silvestres.

CONSERVACION DISPERSION DIVERSIDAD GENETICA FUERZA EVOLUTIVA SELECCION ARTIFICIAL BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA BIOLOGÍA VEGETAL (BOTÁNICA) ECOLOGÍA VEGETAL ECOLOGÍA VEGETAL

Trade-offs in the genetic control of functional and nutritional quality traits in UK winter wheat

Nick Fradgley Keith Gardner Stéphanie M. Swarbreck Alison Bentley (2022, [Artículo])

CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA WHEAT GENETIC CONTROL NUTRITIVE VALUE QUANTITATIVE TRAIT LOCI

Improving hybrid rice breeding programs via stochastic simulations: number of parents, number of hybrids, tester update, and genomic prediction of hybrid performance

Roberto Fritsche-Neto Marlee Labroo (2024, [Artículo])

Genomic Prediction Reciprocal Recurrent Selection Heterotic Pools CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA STOCHASTIC MODELS RICE HYBRIDS GENETIC IMPROVEMENT GENETIC GAIN BREEDING PROGRAMMES

Genetic structure analysis and identifying key founder inbred lines in diverse elite sub-tropical maize inbred lines

Melaku Gedil Ana Luisa Garcia-Oliveira Nnanna Unachukwu Cesar Petroli Sarah Hearne Abebe Menkir (2023, [Artículo])

Genetic Relationship Desirable Target Traits Parental Selection CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA GENETIC STRUCTURES INBRED LINES MAIZE BREEDING PROGRAMMES

Association of specific expansins with growth in maize leaves is maintained under environmental, genetic, and developmental sources of variation

Francois Tardieu (2007, [Artículo])

Environmental Stimuli Expansins CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA CELLS CROPS GENETIC ENGINEERING PROTEINS TISSUE EPIDERMIS ZEA MAYS

Emmer wheat eco-geographic and genomic congruence shapes phenotypic performance under mediterranean climate

Dana Fuerst SHAILESH YADAV Rajib Roychowdhury Carolina Sansaloni Sariel Hübner (2022, [Artículo])

Emmer Wheat CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA WHEAT GENETIC VARIATION CLIMATE PHENOLOGY YIELDS MEDITERRANEAN CLIMATE

Exploring the agronomic performance and molecular characterization of diverse spring durum wheat germplasm in Kazakhstan

Kadyrzhan Mukin Abdelfattah DABABAT Timur Savin Vladimir Shamanin Vadim Yusov Alexey Morgounov (2023, [Artículo])

Germplasm Characterization Molecular Markers CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA TRITICUM TURGIDUM GERMPLASM GENETIC MARKERS BREEDING

Indian wheat genomics initiative for harnessing the potential of wheat germplasm resources for breeding disease-resistant, nutrient-dense, and climate-resilient cultivars

Sundeep Kumar Reyaz Mir Pawan Kulwal UTTAM KUMAR suneel kumar Shailendra Sharma Ravinder Singh Amit Singh Dr. Subhash Bhardwaj Manoj Prasad Kuldeep Singh (2022, [Artículo])

Indian Wheat Genomics Initiative Genomic Selection CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA WHEAT GENETIC RESOURCES MARKER-ASSISTED SELECTION GENE BANKS STRESS ABIOTIC STRESS BIOTIC STRESS