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Distance learning for farmers: Experience during the pandemic

Andrea Gardeazabal (2023, [Documento de trabajo])

In response to the COVID-19 pandemic's disruption of farmer training—a crucial component for enhancing the resilience and livelihoods of smallholder farmers—CIMMYT innovated educational solutions to sustain capacity building in agri-food systems. Addressing the challenges of limited mobile device access, poor internet connectivity, and digital illiteracy, CIMMYT implemented two pilot projects in Mexico. These projects facilitated distance learning for adult farmers in rural areas, employing both internet-based and non-internet methods. The non-internet approach utilized traditional media like print, while the internet-based approach leveraged WhatsApp for educational content delivery. Building on these experiences, CIMMYT expanded its offerings by creating micro -courses delivered through WhatsApp, hosted on the Co-LAB's new Learning Network platform, specifically targeting farmers. This paper delves into the various strategies, methods, and techniques adopted, documenting the learning outcomes, results, and key conclusions drawn from these innovative training initiatives.

Distance Learning Digital Inclusion Innovative Training CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA DISTANCE EDUCATION CAPACITY DEVELOPMENT METHODS COMMUNICATION TECHNOLOGY

Universal fixation system for pad printing of plastic parts

JOSE ALEJANDRO FERNANDEZ RAMIREZ (2023, [Artículo])

Pad printing is used in automotive, medical, electrical and other industries, employing diverse materials to transfer a 2D image onto a 3D object with different sizes and geometries. This work presents a universal fixation system for pad printing of plastic parts (UFSP4) in response to the needs of small companies that cannot afford to invest in the latest technological advances. The UFSP4 comprises two main subsystems: a mechanical support system (i.e., support structure, jig matrix and braking system) and a control system (i.e., an electronic system and an electric-hydraulic system). A relevant feature is the combination of a jig matrix and jig pins to fixate complex workpieces with different sizes. Using finite element analysis (FEA), in the mesh convergence, the total displacement converges to 0.00028781 m after 12,000 elements. The maximum equivalent stress value is 1.22 MPa for the polycarbonate plate in compliance with the safety factor. In a functionality test of the prototype performed in a production environment for one hour, the jigs fixed by the plate did not loosen, maintaining the satisfactory operation of the device. This is consistent with the displacement distribution of the creep analysis and shows the absence of the creep phenomenon. Based on FEA that underpinned the structural health computation of the braking system, the prototype was designed and built, seeking to ensure a reliable and safe device to fixate plastic parts, showing portability, low-cost maintenance and adaptability to the requirements of pad printing of automotive plastic parts.

Use of AI tools declaration: The authors declare they have not used Artificial Intelligence (AI) tools in the creation of this article.

Acknowledgments: To the company Plásticos Decorados and Centro de Tecnología Avanzada (CIATEQ A.C.) for the facilities and support to carry out this work. To the Consejo Nacional de Humanidades, Ciencias y Tecnologías (CONAHCYT) for a Ph.D. scholarship support number 500839 and CONAHCYT SNI.

Conflict of interest: The authors declare there are no conflicts of interest.

Modular fixtures Jig matrix Prototype design Pad printing Fixture methods INGENIERÍA Y TECNOLOGÍA CIENCIAS TECNOLÓGICAS OTRAS ESPECIALIDADES TECNOLÓGICAS OTRAS OTRAS

On-farm storage loss estimates of maize in Kenya using community survey methods

Hugo De Groote Anani Bruce (2023, [Artículo])

Maize is the most important staple in sub-Saharan Africa (SSA), with highly seasonal production. High storage losses affect food security, but good estimations are lacking. A new method using focus group discussions (FGDs) was tested with 121 communities (1439 farmers, 52% women) in Kenya's six maize-growing zones, to estimate the maize losses to storage pests and analyze farmer practices. As control strategies, half of the farmers used chemical pesticides (49%), while hermetic bags (16%) and botanicals (15%) were also popular. Relative loss from weevils in the long rains was estimated at 23%, in the short rains 18%, and annually 21%. Fewer farmers were affected by the larger grain borer (LGB) than by maize weevils: 42% in the long rainy season and 32% in the short rainy season; losses from LGB were also smaller: 19% in the long season, 17% in the short season, and 18% over the year. Total storage loss, from both species combined, was estimated at 36%, or 671,000 tonnes per year. The greatest losses occur in the humid areas, especially the moist mid-altitudes (56%), and with smaller loss in the drylands (20–23%). Extrapolating the point data and overlaying with the maize production map shows the geographic distribution of the losses, with the most important area found around Lake Victoria. FGDs provide convenient and cheap tools to estimate storage losses in representative communities, but a total loss estimate of 36% is higher than is found in other studies, so its accuracy and framing effects need to be assessed. We conclude that storage pests remain a major problem, especially in western Kenya, and that the use of environmentally friendly technologies such as hermetic storage and botanicals needs more attention, both by the public extension service and private agrodealers.

Larger Grain Borer Maize Weevil CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA MAIZE STORAGE LOSSES PESTS SURVEY METHODS

The banana MaWRKY18, MaWRKY45, MaWRKY60 and MaWRKY70 genes encode functional transcription factors and display differential expression in response to defense phytohormones

SERGIO GARCIA LAYNES VIRGINIA AURORA HERRERA VALENCIA Lilia Guadalupe Tamayo Torres VERONICA LIMONES BRIONES FELIPE ALONSO BARREDO POOL FRAY MARTIN BAAS ESPINOLA Angel Alpuche-Solis CARLOS ALBERTO PUCH HAU SANTY PERAZA ECHEVERRIA (2022, [Artículo])

WRKY transcription factors (TFs) play key roles in plant defense responses through phytohormone signaling pathways. However, their functions in tropical fruit crops, especially in banana, remain largely unknown. Several WRKY genes from the model plants rice (OsWRKY45) and Arabidopsis (AtWRKY18, AtWRKY60, AtWRKY70) have shown to be attractive TFs for engineering disease resistance. In this study, we isolated four banana cDNAs (MaWRKY18, MaWRKY45, MaWRKY60, and MaWRKY70) with homology to these rice and Arabidopsis WRKY genes. The MaWRKY cDNAs were isolated from the wild banana Musa acuminata ssp. malaccensis, which is resistant to several diseases of this crop and is a progenitor of most banana cultivars. The deduced amino acid sequences of the four MaWRKY cDNAs revealed the presence of the conserved WRKY domain of ~60 amino acids and a zinc-finger motif at the N-terminus. Based on the number of WRKY repeats and the structure of the zinc-finger motif, MaWRKY18 and MaWRKY60 belong to group II of WRKY TFs, while MaWRKY45 and MaWRKY70 are members of group III. Their corresponding proteins were located in the nuclei of onion epidermal cells and were shown to be functional TFs in yeast cells. Moreover, expression analyses revealed that the majority of these MaWRKY genes were upregulated by salicylic acid (SA) or methyl jasmonate (MeJA) phytohormones, although the expression levels were relatively higher with MeJA treatment. The fact that most of these banana WRKY genes were upregulated by SA or MeJA, which are involved in systemic acquired resistance (SAR) or induced systemic resistance (ISR), respectively, make them interesting candidates for bioengineering broad-spectrum resistance in this crop. © 2022 by the authors.

BANANA TRANSCRIPTION FACTOR WRKY DEFENSE PHYTOHORMONES SALICYLIC ACID METHYL JASMONATE SAR ISR BROAD-SPECTRUM RESISTANCE BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA GENÉTICA GENÉTICA MOLECULAR DE PLANTAS GENÉTICA MOLECULAR DE PLANTAS

The Banana MaWRKY18, MaWRKY45, MaWRKY60 and MaWRKY70 Genes Encode Functional Transcription Factors and Display Differential Expression in Response to Defense Phytohormones

SERGIO GARCIA LAYNES VIRGINIA AURORA HERRERA VALENCIA Lilia Guadalupe Tamayo Torres VERONICA LIMONES BRIONES FELIPE ALONSO BARREDO POOL FRAY MARTIN BAAS ESPINOLA Ángel Gabriel Alpuche Solís CARLOS ALBERTO PUCH HAU SANTY PERAZA ECHEVERRIA (2022, [Artículo])

"WRKY transcription factors (TFs) play key roles in plant defense responses through phytohormone signaling pathways. However, their functions in tropical fruit crops, especially in banana, remain largely unknown. Several WRKY genes from the model plants rice (OsWRKY45) and Arabidopsis (AtWRKY18, AtWRKY60, AtWRKY70) have shown to be attractive TFs for engineering disease resistance. In this study, we isolated four banana cDNAs (MaWRKY18, MaWRKY45, MaWRKY60, and MaWRKY70) with homology to these rice and Arabidopsis WRKY genes. The MaWRKY cDNAs were isolated from the wild banana Musa acuminata ssp. malaccensis, which is resistant to several diseases of this crop and is a progenitor of most banana cultivars. The deduced amino acid sequences of the four MaWRKY cDNAs revealed the presence of the conserved WRKY domain of ~60 amino acids and a zinc-finger motif at the N-terminus. Based on the number of WRKY repeats and the structure of the zinc-finger motif, MaWRKY18 and MaWRKY60 belong to group II of WRKY TFs, while MaWRKY45 and MaWRKY70 are members of group III. Their corresponding proteins were located in the nuclei of onion epidermal cells and were shown to be functional TFs in yeast cells. Moreover, expression analyses revealed that the majority of these MaWRKY genes were upregulated by salicylic acid (SA) or methyl jasmonate (MeJA) phytohormones, although the expression levels were relatively higher with MeJA treatment. The fact that most of these banana WRKY genes were upregulated by SA or MeJA, which are involved in systemic acquired resistance (SAR) or induced systemic resistance (ISR), respectively, make them interesting candidates for bioengineering broad-spectrum resistance in this crop."

Banana Transcription factor WRKY Defense phytohormones Salicylic acid Methyl jasmonate SAR ISR Broad-spectrum resistance BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA GENÉTICA GENÉTICA