Enviromic prediction enables the characterization and mapping of Eucalyptus globulus Labill breeding zones

Germano Costa Neto Jose Crossa (2024, [Artículo])

Forest Tree Breeding Genomic Relationship Matrix Genomic Selection Best Linear Unbiased Prediction CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA FOREST TREES BREEDING MARKER-ASSISTED SELECTION MYRTACEAE EUCALYPTUS GLOBULUS

Efficacy of plant breeding using genomic information

Osval Antonio Montesinos-Lopez Alison Bentley Carolina Saint Pierre Leonardo Abdiel Crespo Herrera Morten Lillemo Jose Crossa (2023, [Artículo])

Genomic Selection Genomic Prediction Genomic Best Linear Unbiased Predictor CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA PLANT BREEDING GENOMICS MARKER-ASSISTED SELECTION ENVIRONMENT

A novel method for genomic-enabled prediction of cultivars in new environments

Osval Antonio Montesinos-Lopez Brandon Alejandro Mosqueda González Jose Crossa (2023, [Artículo])

Genomic Best Linear Unbiased Prediction Gains in Accuracy Genomic Prediction Novel Methods CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA GENOTYPE ENVIRONMENT INTERACTION METHODS ENVIRONMENT

Hacia el desarrollo de un nuevo antiparasitario basado en células en suspensión de Carica papaya

CYNTHIA ALEJANDRA GUZMÁN MEDINA (2023, [Tesis de doctorado])

Las enfermedades parasitarias son un problema de salud pública que afectan a más de la

quinta parte de la población a nivel mundial. Además de la morbilidad que generan, sino

son tratadas y controladas de manera adecuada pueden llegar a causar la muerte.

En el mercado existen diferentes antiparasitarios comerciales, que si bien son efectivos,

presentan diferentes efectos adversos tanto para el individuo parasitado (i.e. destrucción

de la microbiota intestinal) como para el medio ambiente. Este panorama señala la

relevancia de desarrollar tratamientos antiparasitarios más amigables con el ambiente y con

menores efectos adversos que estén disponibles actualmente en el mercado. Productos

obtenidos a partir de plantas con propiedades anti-parasitarias, cultivadas in vitro en

condiciones controladas, podrían representar una alternativa realista. Entre ellas figura la

planta Carica papaya, especie de zonas tropicales y subtropicales del planeta a cuyos frutos

se les adjudica actividades antiparasitarias. Estas propiedades se atribuyen a algunos de

sus compuestos concentrados en el latex y en las semillas como la papaina, la

quimiopapaína, la lisozima, la glicil-endopeptidasa, la proteasa de cisteína y el benzil-

isotiocinato.

El objetivo del presente trabajo fue evaluar la actividad antiparasitaria in vitro e in vivo de

un conjunto de callos y líneas de células de papaya de Carica papaya contra cisticercos de

Taenia crassiceps (cestodo) y contra trofozoitos de Entamoeba histolytica (protozoario). Los

extractos acuosos obtenidos a partir de callos y células cultivados en placa o en suspensión,

respectivamente, de las diferentes líneas de papaya revelaron alta capacidad cisticida

(>98% de mortalidad) in vitro contra cisticercos de Taenia crassiceps. En la evaluación in

vivo la línea no transformada obtuvo mayor efecto cisticida a 10mg/mL reduciendo el

número de cisticercos gemantes y aumentando los cisticercos calcificados en niveles

similares a los obtenidos utilizando antiparasitarios comerciales (albendazol y niclosamida).

Las líneas transformadas y sin transformar de papaya evaluadas indujeron una alta

capacidad amebicida (97% de mortalidad) in vitro contra trofozoitos de Entamoeba

histolytica. Las diferentes líneas transformadas y sin transformar de papaya redujeron

significativamente el número de abscesos amebianos y previnieron la hepatomegalia de

manera no significativamente diferente que el anti-parasitario comercial (metronidazol).

Los resultados generados en el presente estudio señalan al extracto acuoso obtenido a

partir de cultivos en suspensión de células de papaya transformada y no transformada

indujeron alto nivel de protección in vitro e in vivo por lo tanto; este es un producto efectivo

para el tratamiento de la cisticercosis y la amebiasis.

Parasitic diseases are a public health problem that affect more than a fifth of the population

worldwide. Additionally, and along with the morbidity the produce, they can cause death if

they are not treated and controlled adequately.

There are different commercial antiparasitic on the market which, although effective,

present different adverse effects both for the individual (i.e., destruction of the intestinal

microbiota) and for the environment alike. This panorama points out the relevance of

developing more environmentally friendly antiparasitic treatments with fewer adverse effects

than the ones currently available on the market. Products obtained from plants with anti-

parasitic properties, grown in vitro under controlled conditions, could represent a realistic

alternative. Among them is the Carica papaya, a species from tropical and subtropical areas

of the planet whose fruits are believed to have antiparasitic activities. These properties are attributed to some of its compounds concentrated in the latex and seeds such as papain,

chymopapain, lysozyme, glycyl-endopeptidase, cysteine-proteinase and benzyl-

isothiocyanate.

The objective of the present work is to evaluate the in vitro and in vivo antiparasitic activity

of a set of papaya callus and cell lines from Carica papaya against cysticerci of Taenia

crassiceps (cestode) and against trophozoites of Entamoeba histolytica (protozoan). The

aqueous extracts obtained from callus and cells cultured in plates or in suspension,

respectively, of the different papaya lines revealed high cysticidal capacity (>98% mortality)

in vitro against Taenia crassiceps cysticerci. Meanwhile, in the in vivo evaluation, the non-

transformed line obtained a greater cysticidal effect at 10 mg/mL, reducing the number of

budding cysticerci and increasing calcified cysticerci at levels like those obtained using

commercial antiparasitic (albendazole and niclosamide). The papaya lines evaluated

induced a high amoebicidal capacity (97% mortality) in vitro against Entamoeba histolytica

trophozoites. The different papaya lines significantly reduced the number of amoebic

abscesses and prevented hepatomegaly in a non-significantly different manner than the

commercial anti-parasitic (metronidazole).

The results produced in this thesis indicate that the aqueous extract obtained from

suspension cultures of transformed and non-transformed papaya cells induced a high level

of protection in vitro and in vivo. Therefore, this may represent an effective product for the

treatment of cysticercosis and amoebiasis.

CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA CIENCIAS AGRARIAS Carica papaya, antiparasitario, células en suspensión, T. crassiceps, E. histolytica. Carica papaya, antiparasitic, in-suspension cells, T. crassiceps, E. histolytica.

Análisis de la implementación de los lineamientos técnicos específicos para la reapertura de las actividades económicas de las pequeñas empresas en Cuernavaca, Morelos

DIANA LAURA HERNÁNDEZ CARLOS (2023, [Tesis de maestría])

El presente trabajo habla de cómo afecto la pandemia por COVID 19 a las pequeñas y medianas empresas de Cuernavaca, Morelos, y las medidas de prevención que las autoridades de salud emitieron en qué consisten, cómo se debían llevar a cabo para garantizar la continuidad de las operaciones de forma segura durante la etapa crítica de la pandemia.

Se analiza la implementación por parte de estas pequeñas y medianas empresas, y el cómo fueron informados y capacitados para ello, así como los recursos con los que contaron.

CIENCIAS SOCIALES CIENCIAS ECONÓMICAS Pandemia, lineamiento, reapertura, conocimiento, implementación. recursos

Phylogenetic relationships of Pseudo-nitzschia subpacifica (Bacillariophyceae) from the Mexican Pacific, and its production of domoic acid in culture

Sonia Quijano (2020, [Artículo])

Pseudo-nitzschia is a cosmopolitan genus, some species of which can produce domoic acid (DA), a neurotoxin responsible for the Amnesic Shellfish Poisoning (ASP). In this study, we identified P. subpacifica for the first time in Todos Santos Bay and Manzanillo Bay, in the Mexican Pacific using SEM and molecular methods. Isolates from Todos Santos Bay were cultivated under conditions of phosphate sufficiency and deficiency at 16°C and 22°C to evaluate the production of DA. This toxin was detected in the particulate (DAp) and dissolved (DAd) fractions of the cultures during the exponential and stationary phases of growth of the cultures. The highest DA concentration was detected during the exponential phase grown in cells maintained in P-deficient medium at 16°C (1.14 ± 0.08 ng mL-1 DAd and 4.71 ± 1.11 × 10−5 ng cell-1 of DAp). In P-sufficient cultures DA was higher in cells maintained at 16°C (0.25 ± 0.05 ng mL-1 DAd and 9.41 ± 1.23 × 10−7 ng cell-1 of DAp) than in cells cultured at 22°C. Therefore, we confirm that P. subpacifica can produce DA, especially under P-limited conditions that could be associated with extraordinary oceanographic events such as the 2013–2016 "Blob" in the northeastern Pacific Ocean. This event altered local oceanographic conditions and possibly generated the presence of potential harmful species in areas with economic importance on the Mexican Pacific coast. © 2020 Quijano-Scheggia et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

domoic acid, domoic acid, kainic acid, Article, cell growth, controlled study, diatom, Mexico, morphology, nonhuman, Pacific Ocean, phylogeny, plant cell, plant growth, Pseudo nitzschia, toxin analysis, cell culture technique, classification, diatom, CIENCIAS FÍSICO MATEMÁTICAS Y CIENCIAS DE LA TIERRA CIENCIAS DE LA TIERRA Y DEL ESPACIO OCEANOGRAFÍA OCEANOGRAFÍA

Solanum tuberosum Microtuber Development under Darkness Unveiled through RNAseq Transcriptomic Analysis

ELIANA VALENCIA LOZANO LISSET HERRERA ISIDRON Osiel Salvador Recoder-Meléndez Aarón Barraza Celis JOSE LUIS CABRERA PONCE (2022, [Artículo])

"Potato microtuber (MT) development through in vitro techniques are ideal propagules for producing high quality potato plants. MT formation is influenced by several factors, i.e., photoperiod, sucrose, hormones, and osmotic stress. We have previously developed a protocol of MT induction in medium with sucrose (8% w/v), gelrite (6g/L), and 2iP as cytokinin under darkness. To understand the molecular mechanisms involved, we performed a transcriptome-wide analysis. Here we show that 1715 up- and 1624 down-regulated genes were involved in this biological process. Through the protein–protein interaction (PPI) network analyses performed in the STRING database (v11.5), we found 299 genes tightly associated in 14 clusters. Two major clusters of up-regulated proteins fundamental for life growth and development were found: 29 ribosomal proteins (RPs) interacting with 6 PEBP family members and 117 cell cycle (CC) proteins. The PPI network of up-regulated transcription factors (TFs) revealed that at least six TFs–MYB43, TSF, bZIP27, bZIP43, HAT4 and WOX9–may be involved during MTs development. The PPI network of down-regulated genes revealed a cluster of 83 proteins involved in light and photosynthesis, 110 in response to hormone, 74 in hormone mediate signaling pathway and 22 related to aging."

transcriptome-wide analysis, microtubers, potato, Solanum tuberosum, darkness, cell cycle, ribosomal proteins, PEBP family genes, cytokinin BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA GENÉTICA GENÉTICA MOLECULAR DE PLANTAS GENÉTICA MOLECULAR DE PLANTAS

XYZ Micropositioning System Based on Compliance Mechanisms Fabricated by Additive Manufacturing

CARLOS ANDRES FERRARA BELLO Pedro Vargas Chable José Gerardo Vera Dimas Margarita Tecpoyotl Torres (2021, [Artículo])

This article presents the design and implementation of a micropositioning system actuated

by three piezoelectric stacks to control its displacements on XYZ axes. The use of conventional

piezoelectric buzzers allows us to reduce fabrication costs. The working or mobile platform is the base

for objects that will be manipulated, for example, in automated assembling. The micropositioner can

be integrated into a microgripper to generate a complete manipulation system. For micropositioner

fabrication, at first, Polylactic Acid (PLA) was chosen as the structural material, but after simulation

and some experimental tests performed with a micropositioner made of Acrylonitrile Butadiene

Styrene (ABS), it showed larger displacement (approx. 20%) due to its lower stiffness. A third test

was performed with a positioner made with Polyethylene Terephthalate Glycol (PETG), obtaining an

intermediate performance. The originality of this work resides in the geometrical arrangement based

on thermoplastic polymer compliance mechanisms, as well as in the use of additive manufacturing

to fabricate it. An experimental setup was developed to carry out experimental tests. ANSYS™ was

used for simulation.

INGENIERÍA Y TECNOLOGÍA CIENCIAS TECNOLÓGICAS PETG, MEMS, micromanipulation, piezoelectric actuation, hysteresis effect linearization

Maintenance of Coastal Surface Blooms by Surface Temperature Stratification and Wind Drift

MARY CARMEN RUIZ DE LA TORRE (2013, [Artículo])

Algae blooms are an increasingly recurrent phenomenon of potentially socio-economic impact in coastal waters globally and in the coastal upwelling region off northern Baja California, Mexico. In coastal upwelling areas the diurnal wind pattern is directed towards the coast during the day. We regularly found positive Near Surface Temperature Stratification (NSTS), the resulting density stratification is expected to reduce the frictional coupling of the surface layer from deeper waters and allow for its more efficient wind transport. We propose that the net transport of the top layer of approximately 2.7 kilometers per day towards the coast helps maintain surface blooms of slow growing dinoflagellate such as Lingulodinium polyedrum. We measured: near surface stratification with a free-rising CTD profiler, trajectories of drifter buoys with attached thermographs, wind speed and direction, velocity profiles via an Acoustic Doppler Current Profiler, Chlorophyll and cell concentration from water samples and vertical migration using sediment traps. The ADCP and drifter data agree and show noticeable current shear within the first meters of the surface where temperature stratification and high cell densities of L. polyedrum were found during the day. Drifters with 1m depth drogue moved towards the shore, whereas drifters at 3 and 5 m depth showed trajectories parallel or away from shore. A small part of the surface population migrated down to the sea floor during night thus reducing horizontal dispersion. The persistent transport of the surface bloom population towards shore should help maintain the bloom in favorable environmental conditions with high nutrients, but also increasing the potential socioeconomic impact of the blooms. The coast wise transport is not limited to blooms but includes all dissolved and particulate constituents in surface waters. © 2013 Ruiz-de la Torre et al.

chlorophyll, algal bloom, article, cell count, cell density, coastal waters, controlled study, dinoflagellate, Lingulodinium polyedrum, meteorological phenomena, Mexico, near surface temperature stratification, nonhuman, nutrient concentration, popul CIENCIAS FÍSICO MATEMÁTICAS Y CIENCIAS DE LA TIERRA CIENCIAS DE LA TIERRA Y DEL ESPACIO OCEANOGRAFÍA OCEANOGRAFÍA

Diseño de arreglos de antenas para entornos de 5G utilizando técnicas de simplificación en la red de alimentación

Design of antenna arrays for 5G environments using simplification techniques in the feeding network

ELIZVAN JUAREZ PACHECO (2023, [Tesis de doctorado])

En los últimos años, las redes de comunicaciones inalámbricas de quinta generación (5G) han tomado gran relevancia debido al crecimiento del número de usuarios móviles que se conectan a estas redes inalámbricas. Estas redes utilizan arreglos de antenas para generar haces de radiación directivos que pueden escanearse en una o múltiples direcciones en el espacio mediante el control de una red de alimentación. En una red de alimentación convencional cada elemento de antena se alimenta con un dispositivo amplificador y desfasador, lo que resulta en sistemas costosos y complejos de implementar. Por lo tanto, esta tesis de investigación propone nuevas técnicas de diseño que simplifican la red de alimentación al reducir el número de puertos de entrada y dispositivos desfasadores necesarios en el sistema de antenas. Las configuraciones propuestas consideran como requisitos de diseño el nivel de lóbulo lateral (SLL por sus siglas en inglés), rango de escaneo del haz principal y ancho de banda de operación adecuados para sistemas de 5G. Así, se introduce la técnica de bloques CORPS (Estructuras Periódicas de Radiación Coherente, en inglés) como una solución para simplificar la red de alimentación en arreglos lineales y planares. Esta técnica aprovecha la propiedad de interpolación de fase de las redes CORPS de una capa para generar los valores cofasales ideales necesarios para escanear el haz principal. Además, la aplicación de una excitación de amplitud de coseno alzado genera un haz de radiación con bajo SLL. Adicionalmente, se aplica la técnica de bloques CORPS en configuraciones con subarreglos para mejorar la reducción de desfasadores en comparación con la implementación individual de cada tecnología. Los resultados obtenidos mediante simulación electromagnética y mediciones experimentales validan los diferentes diseños propuestos. Todo esto contribuye al estado del arte al presentar diferentes diseños de arreglos de antenas que simplifican la red de alimentación manteniendo buenas características en el patrón de radiación en comparación con diseños tradicionales.

In recent years, fifth-generation wireless networks (5G) have become very relevant due to the exponential growth in the number of mobile users connecting to wireless networks. These networks employ antenna arrays to generate directional radiation beams that can be scanned in one or multiple directions in space by controlling a feeding network. In a conventional feeding network, each antenna element is fed with an amplifier and phase shifter device, which results in expensive and complex systems to implement. Therefore, this thesis proposes novel design techniques that simplify the feeding network by reducing the number of input ports and phase shifter devices required in the antenna system. The proposed configurations take into account requirements such as side lobe level (SLL), scanning range, and bandwidth appropriate for 5G systems. Thus, the CORPS (Coherent Radiation Periodic Structures) blocks technique is introduced as a solution to simplify the feeding network in linear and planar phased arrays. This technique takes advantage of the phase interpolation property of single-layer CORPS to generate the ideal cophasal values necessary for the main beam scanning. Furthermore, the application of a raised-cosine amplitude distribution generates a radiation beam with low SLL. Additionally, the CORPS blocks technique is applied in subarrays configurations to improve the phase shifters reduction compared to the individual implementation of each technology. The results obtained by electromagnetic simulation and experimental measurements validate the different proposed designs. This contributes to the state of the art by different designs of antenna arrays that simplify the feeding network while maintaining good radiation pattern characteristics when compared to traditional designs.

Arreglo de antenas, Red de alimentación, Quinta generación, Arreglo lineal, Arreglo planar Antenna array, Feeding network, Fifth Generation, Linear array, Planar array INGENIERÍA Y TECNOLOGÍA CIENCIAS TECNOLÓGICAS TECNOLOGÍA DE LAS TELECOMUNICACIONES ANTENAS ANTENAS