Coronin is a component of the endocytic collar of hyphae of neurospora crassa and is necessary for normal growth and morphogenesis

RAMON OSVALDO ECHAURI ESPINOSA (2012, [Artículo])

Coronin plays a major role in the organization and dynamics of actin in yeast. To investigate the role of coronin in a filamentous fungus (Neurospora crassa), we examined its subcellular localization using fluorescent proteins and the phenotypic consequences of coronin gene (crn-1) deletion in hyphal morphogenesis, Spitzenkörper behavior and endocytosis. Coronin-GFP was localized in patches, forming a subapical collar near the hyphal apex; significantly, it was absent from the apex. The subapical patches of coronin colocalized with fimbrin, Arp2/3 complex, and actin, altogether comprising the endocytic collar. Deletion of crn-1 resulted in reduced hyphal growth rates, distorted hyphal morphology, uneven wall thickness, and delayed establishment of polarity during germination; it also affected growth directionality and increased branching. The Spitzenkörper of Δcrn-1 mutant was unstable; it appeared and disappeared intermittently giving rise to periods of hyphoid-like and isotropic growth respectively. Uptake of FM4-64 in Δcrn-1 mutant indicated a partial disruption in endocytosis. These observations underscore coronin as an important component of F-actin remodeling in N. crassa. Although coronin is not essential in this fungus, its deletion influenced negatively the operation of the actin cytoskeleton involved in the orderly deployment of the apical growth apparatus, thus preventing normal hyphal growth and morphogenesis. © 2012 Echauri-Espinosa et al.

actin related protein 2-3 complex, F actin, fimbrin protein, fluorescent dye, fungal protein, fungal protein coronin, green fluorescent protein, unclassified drug, actin binding protein, coronin proteins, fungal protein, article, cell polarity, contr BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA MICROBIOLOGÍA MICROBIOLOGÍA

Maize ZmBES1/BZR1-3 and-9 transcription factors negatively regulate drought tolerance in transgenic arabidopsis

Fuai Sun XUECAI ZHANG Haoqiang Yu (2022, [Artículo])

BZR1s CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA ARABIDOPSIS DNA BINDING PROTEINS PLANT PROTEIN TRANSCRIPTION FACTORS DROUGHT GENE EXPRESSION REGULATION GENETICS MAIZE METABOLISM TRANSGENIC PLANTS ABIOTIC STRESS

Novel mutant alleles reveal a role of the extra-large G protein in rice grain filling, panicle architecture, plant growth, and disease resistance

Akshaya Biswal Daisuke Urano (2022, [Artículo])

Heterotrimeric G Proteins Extra-Large G Proteins Cas9 OsXLG CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA CRISPR RICE PROTEINS PLANT GROWTH DISEASE RESISTANCE

Thermal and mechanical properties of PLA-based multiscale cellulosic biocomposites

MIGUEL ANGEL RUZ CRUZ Pedro Jesús Herrera Franco Emmanuel Alejandro Flores Johnson MARIA VERONICA MORENO CHULIM LUCIANO MIGUEL GALERA MANZANO Alex Valadez González (2022, [Artículo])

In this work polylactic acid (PLA) based multiscale cellulosic biocomposites were prepared with the aim to evaluate the effect of the incorporation of cellulose nanocrystals (CNCs) on the PLA biocomposites reinforced with cellulose microfibers (MFCs). For this, PLA composite materials reinforced with both MFCs and with a combination of MFCs and CNCs were prepared, while keeping the content of cellulosic reinforcements constant. The thermal and mechanical properties of these multiscale PLA biocomposites were characterized by thermogravimetry (TGA), differential scanning calorimetry (DSC), flexural mechanical and, dynamic mechanical (DMA) tests. Likewise, they were characterized by Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). The results show that the replacement of MFCs by CNCs in the 1–5% range appreciably modifies the thermal and mechanical properties of multiscale compounds. For example, they increase the thermal stability of the materials, modify the PLA crystallization process and play the role of adhesion promoters since the mechanical properties in flexure increase in the order of 40% and the storage modulus increases in the order of 35% at room temperature. Also, the addition of CNCs increases the relaxation temperature of the material from 50 to 60 °C, thereby expanding the temperature range for its use. © 2022 The Author(s)

MULTISCALE BIOCOMPOSITES CELLULOSE MICROFIBER CELLULOSE NANOCRYSTALS HIERARCHICAL STRUCTURE PROPERTIES INGENIERÍA Y TECNOLOGÍA CIENCIAS TECNOLÓGICAS TECNOLOGÍA DE MATERIALES PROPIEDADES DE LOS MATERIALES PROPIEDADES DE LOS MATERIALES

Transcriptome mining provides insights into cell wall metabolism and fiber lignification in Agave tequilana Weber

Luis Fernando Maceda Lopez ELSA BEATRIZ GONGORA CASTILLO Enrique Ibarra-Laclette DALIA C. MORAN VELAZQUEZ AMARANTA GIRON RAMIREZ Matthieu Bourdon José Luis Villalpando Aguilar Gabriela Chavez-Calvillo Toomer John Tang Parastoo Azadi Jorge Manuel Santamaría Fernández Itzel López-Rosas Mercedes G Lopez June Simpson FULGENCIO ALATORRE COBOS (2022, [Artículo])

Resilience of growing in arid and semiarid regions and a high capacity of accumulating sugar-rich biomass with low lignin percentages have placed Agave species as an emerging bioen-ergy crop. Although transcriptome sequencing of fiber-producing agave species has been explored, molecular bases that control wall cell biogenesis and metabolism in agave species are still poorly understood. Here, through RNAseq data mining, we reconstructed the cellulose biosynthesis pathway and the phenylpropanoid route producing lignin monomers in A. tequilana, and evaluated their expression patterns in silico and experimentally. Most of the orthologs retrieved showed differential expression levels when they were analyzed in different tissues with contrasting cellulose and lignin accumulation. Phylogenetic and structural motif analyses of putative CESA and CAD proteins allowed to identify those potentially involved with secondary cell wall formation. RT-qPCR assays revealed enhanced expression levels of AtqCAD5 and AtqCESA7 in parenchyma cells associated with extraxylary fibers, suggesting a mechanism of formation of sclerenchyma fibers in Agave similar to that reported for xylem cells in model eudicots. Overall, our results provide a framework for un-derstanding molecular bases underlying cell wall biogenesis in Agave species studying mechanisms involving in leaf fiber development in monocots. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.

AGAVE CELL WALLS LIGNOCELLULOSE CAD PROTEIN CESA PROTEIN SCLERENCHYMA BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA GENÉTICA GENÉTICA MOLECULAR DE PLANTAS GENÉTICA MOLECULAR DE PLANTAS

Chapter 11. Genomic insights on global journeys of adaptive wheat genes that brought us to modern wheat

deepmala sehgal Laura Dixon Diego Pequeno Jose Crossa Alison Bentley Susanne Dreisigacker (2024, [Capítulo de libro])

Hexaploid Wheat Adaptive Genes Novel Genomic Regions Gene-Based Modeling Process-Based Modeling Global Food Security CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA HEXAPLOIDY WHEAT QUANTITATIVE TRAIT LOCI MODELLING FOOD SECURITY

Análisis de velocidad de soldadura robotizada para proceso MIG en acero1045 AISI

Carlos Eduardo Hernández Acero (2022, [Tesis de maestría])

Esta investigación denominada “análisis de velocidad de soldadura robotizada para proceso MIG en acero 1045 AISI” busca el desarrollo de un modelo matemático que permita, mediante parámetros conocidos, el cálculo de la penetración de soldadura en piezas unidas con proceso Metal Inert Gas (MIG). El objetivo se centra en buscar la combinación de valores paramétricos para la velocidad de soldadura, el voltaje y la corriente, los que se obtienen mediante la aplicación de un diseño experimental, que, ejecutadas en el proceso, pueda lograr una penetración de soldadura aceptable para la unión de la pieza. El modelo de superficie de respuesta aplicado fue un diseño de experimentos 2k con diseño central compuesto, y posteriormente el modelo se utilizó para estimar la velocidad de soldadura. Este diseño de experimentos se ejecutó por medio del programa estadístico Minitab versión 17. (Hernández Acero et al., 2022). Una vez teniendo la penetración de soldadura deseada en la pieza, se puede calcular la velocidad de soldadura necesaria para el cálculo de tiempo ciclo y utilizar los parámetros definidos de corriente y voltaje. Los valores de estos factores serán útiles para comenzar la programación del robot ya con la celda funcional y reducir el tiempo de arranque en automático (Hernández Acero et al., 2022).

This research, named “robotic welding speed analysis for MIG process in 1045 AISI steel”, seeks to develop a mathematical model that allow, through know parameters, the calculation of welding penetration in parts joined with Metal Inert Gas (MIG) process. The objective is focused on finding the combination of parametric values for robot welding speed, voltage and current, which are obtained through the application of a design of experiments, which, executed in the process, can achieve an acceptable welding penetration for the union of the part. The response surface model applied was a 2k design of experiments with central composite design, and subsequently the model was used to estimate the welding speed. This design of experiments was obtained using the statistical software Minitab version 17. (Hernández Acero et al., 2022). Once having the desired welding penetration in the part, it can calculate the welding speed necessary for the cycle time calculation and use the defined parameters of current and voltage to start programming the robot with the functional cell and reduce the time of startup (Hernández Acero et al., 2022).

Soldadura MIG Penetración Tiempo ciclo Velocidad de soldadura MIG welding Penetration Cycle time Welding speed INGENIERÍA Y TECNOLOGÍA CIENCIAS TECNOLÓGICAS OTRAS ESPECIALIDADES TECNOLÓGICAS OTRAS OTRAS

A comprehensive account of SARS-CoV-2 genome structure, incurred mutations, lineages and COVID-19 vaccination program

Shailendra Sharma deepmala sehgal Apekshita Singh Shailendra Goel SoomNath Raina (2022, [Artículo])

Corona Viruses Genome Structure Novel Mutations Resistance to Vaccines SARS-CoV-2 CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA COVID-19 ANTIBODY FORMATION LIFE CYCLE VACCINATION VACCINES LINEAGE

Advancing the science and practice of ecological nutrient management for smallholder farmers

Sieglinde Snapp (2022, [Artículo])

CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA AGROECOLOGY DEGRADATION NUTRIENT CYCLES NUTRIENT USE EFFICIENCY SOIL ORGANIC MATTER SMALLHOLDERS

The fate of rice crop residues and context-dependent greenhouse gas emissions: Model-based insights from Eastern India

Sonam Sherpa virender kumar Andrew Mcdonald (2024, [Artículo])

Crop residue burning is a common practice in many parts of the world that causes air pollution and greenhouse gas (GHG) emissions. Regenerative practices that return residues to the soil offer a ‘no burn’ pathway for addressing air pollution while building soil organic carbon (SOC). Nevertheless, GHG emissions in rice-based agricultural systems are complex and difficult to anticipate, particularly in production contexts with highly variable hydrologic conditions. Here we predict long-term net GHG fluxes for four rice residue management strategies in the context of rice-wheat cropping systems in Eastern India: burning, soil incorporation, livestock fodder, and biochar. Estimations were based on a combination of Tier 1, 2, and 3 modelling approaches, including 100-year DNDC simulations across three representative soil hydrologic categories (i.e., dry, median, and wet). Overall, residue burning resulted in total direct GHG fluxes of 2.5, 6.1, and 8.7 Mg CO2-e in the dry, median, and wet hydrologic categories, respectively. Relative to emissions from burning (positive values indicate an increase) for the same dry to wet hydrologic categories, soil incorporation resulted in a −0.2, 1.8, or 3.1 Mg CO2-e change in emissions whereas use of residues for livestock fodder increased emissions by 2.0, 2.1, or 2.3 Mg CO2-e. Biochar reduced emissions relative to burning by 2.9 Mg CO2-e in all hydrologic categories. This study showed that the production environment has a controlling effect on methane and, therefore, net GHG balance. For example, wetter sites had 2.8–4.0 times greater CH4 emissions, on average, than dry sites when rice residues were returned to the soil. To effectively mitigate burning without undermining climate change mitigation goals, our results suggest that geographically-target approaches should be used in the rice-based systems of Eastern India to incentivize the adoption of regenerative ‘no burn’ residue management practices.

Soil Carbon Rice Residue Burning Life Cycle Assessment CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA SOIL CARBON RICE LIFE CYCLE GREENHOUSE GASES CLIMATE CHANGE