Solanum tuberosum Microtuber Development under Darkness Unveiled through RNAseq Transcriptomic Analysis

ELIANA VALENCIA LOZANO LISSET HERRERA ISIDRON Osiel Salvador Recoder-Meléndez Aarón Barraza Celis JOSE LUIS CABRERA PONCE (2022, [Artículo])

"Potato microtuber (MT) development through in vitro techniques are ideal propagules for producing high quality potato plants. MT formation is influenced by several factors, i.e., photoperiod, sucrose, hormones, and osmotic stress. We have previously developed a protocol of MT induction in medium with sucrose (8% w/v), gelrite (6g/L), and 2iP as cytokinin under darkness. To understand the molecular mechanisms involved, we performed a transcriptome-wide analysis. Here we show that 1715 up- and 1624 down-regulated genes were involved in this biological process. Through the protein–protein interaction (PPI) network analyses performed in the STRING database (v11.5), we found 299 genes tightly associated in 14 clusters. Two major clusters of up-regulated proteins fundamental for life growth and development were found: 29 ribosomal proteins (RPs) interacting with 6 PEBP family members and 117 cell cycle (CC) proteins. The PPI network of up-regulated transcription factors (TFs) revealed that at least six TFs–MYB43, TSF, bZIP27, bZIP43, HAT4 and WOX9–may be involved during MTs development. The PPI network of down-regulated genes revealed a cluster of 83 proteins involved in light and photosynthesis, 110 in response to hormone, 74 in hormone mediate signaling pathway and 22 related to aging."

transcriptome-wide analysis, microtubers, potato, Solanum tuberosum, darkness, cell cycle, ribosomal proteins, PEBP family genes, cytokinin BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA GENÉTICA GENÉTICA MOLECULAR DE PLANTAS GENÉTICA MOLECULAR DE PLANTAS

Estudio de la proteína Fibrilarina Like (FBLL1)

JANY VALDES MARTINEZ (2023, [Tesis de maestría])

BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA BIOLOGÍA MOLECULAR BIOLOGÍA MOLECULAR DE PLANTAS BIOLOGÍA MOLECULAR DE PLANTAS

Identificación y clonación de genes relacionados a la biosíntesis del ácido betulínico en Pentalinon andrieuxii

ALEXA SHARAI AGUILAR ACEVEDO (2022, [Tesis de maestría])

BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA BIOLOGÍA MOLECULAR BIOLOGÍA MOLECULAR DE PLANTAS BIOLOGÍA MOLECULAR DE PLANTAS

Identificación de patógenos de anfibios en un bosque nublado del noroeste de Honduras

CARLOS AUGUSTO ANDINO GALEANO (2024, [Tesis de maestría])

BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA BIOLOGÍA MOLECULAR BIOLOGÍA MOLECULAR DE PLANTAS BIOLOGÍA MOLECULAR DE PLANTAS

Efecto de componentes virales sobre la actividad ribonucleasa de la fibrilarina

CARLO STEFANO DECLER CARRASCO (2023, [Tesis de doctorado])

BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA BIOLOGÍA MOLECULAR BIOLOGÍA MOLECULAR DE PLANTAS BIOLOGÍA MOLECULAR DE PLANTAS

Localización celular de la síntesis de alcaloides en Argemone mexicana L.

JOSE IGNACIO LAINES HIDALGO (2024, [Tesis de doctorado])

BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA BIOLOGÍA MOLECULAR BIOLOGÍA MOLECULAR DE PLANTAS BIOLOGÍA MOLECULAR DE PLANTAS

Transcriptome mining provides insights into cell wall metabolism and fiber lignification in Agave tequilana Weber

Luis Fernando Maceda Lopez ELSA BEATRIZ GONGORA CASTILLO Enrique Ibarra-Laclette DALIA C. MORAN VELAZQUEZ AMARANTA GIRON RAMIREZ Matthieu Bourdon José Luis Villalpando Aguilar Gabriela Chavez-Calvillo Toomer John Tang Parastoo Azadi Jorge Manuel Santamaría Fernández Itzel López-Rosas Mercedes G Lopez June Simpson FULGENCIO ALATORRE COBOS (2022, [Artículo])

Resilience of growing in arid and semiarid regions and a high capacity of accumulating sugar-rich biomass with low lignin percentages have placed Agave species as an emerging bioen-ergy crop. Although transcriptome sequencing of fiber-producing agave species has been explored, molecular bases that control wall cell biogenesis and metabolism in agave species are still poorly understood. Here, through RNAseq data mining, we reconstructed the cellulose biosynthesis pathway and the phenylpropanoid route producing lignin monomers in A. tequilana, and evaluated their expression patterns in silico and experimentally. Most of the orthologs retrieved showed differential expression levels when they were analyzed in different tissues with contrasting cellulose and lignin accumulation. Phylogenetic and structural motif analyses of putative CESA and CAD proteins allowed to identify those potentially involved with secondary cell wall formation. RT-qPCR assays revealed enhanced expression levels of AtqCAD5 and AtqCESA7 in parenchyma cells associated with extraxylary fibers, suggesting a mechanism of formation of sclerenchyma fibers in Agave similar to that reported for xylem cells in model eudicots. Overall, our results provide a framework for un-derstanding molecular bases underlying cell wall biogenesis in Agave species studying mechanisms involving in leaf fiber development in monocots. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.

AGAVE CELL WALLS LIGNOCELLULOSE CAD PROTEIN CESA PROTEIN SCLERENCHYMA BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA GENÉTICA GENÉTICA MOLECULAR DE PLANTAS GENÉTICA MOLECULAR DE PLANTAS

Potential of Omics to control diseases and pests in the Coconut tree

MIGUEL ALONSO TZEC SIMA Jean Wildort Félix María Inés Granados Alegría Mónica Aparicio Ortiz Dilery Juarez Monroy Damian Mayo Sarai Vivas-Lopez Rufino Gómez-Tah Blondy Beatriz Canto Canché Maxim Berezovski Ignacio Rodrigo Islas Flores (2022, [Artículo])

The coconut palm (Cocos nucifera L.) is a common crop in pantropical areas facing various challenges, one of them being the control of diseases and pests. Diseases such as bud rot caused by Phytophthora palmivora, lethal yellowing caused by phytoplasmas of the types 16SrIV-A, 16SrIV-D or 16SrIV-E, among others, and pests like the coconut palm weevil, Rhynchophorus vulneratus (Coleoptera: Curculionidae), and the horned beetle, Oryctes rhinocerus (Coleoptera: Scarabaeidae), are controlled by applying pesticides, pheromones and cultural control. These practices do not guarantee eradication since some causal agents have become resistant or are imbedded in infected tissues making them difficult to eradicate. This review condenses the current genomics, transcriptomics, proteomics and metabolomics studies which are being conducted with the aim of understanding the pathosystems associated with the coconut palm, highlighting the findings generated by omics studies that may become future targets for the control of diseases and pests in the coconut crop. © 2022 by the authors.

COCOS NUCIFERA L. OMICS PESTS INSECTS DISEASES PATHOGENS BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA BIOLOGÍA MOLECULAR BIOLOGÍA MOLECULAR DE PLANTAS BIOLOGÍA MOLECULAR DE PLANTAS

Análisis de la expresión de genes involucrados en la conversión en planta de embriones somáticos de Capsicum chinense Jacq.

Gema Pijeira Fernández (2020, [Tesis de maestría])

BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA BIOLOGÍA MOLECULAR BIOLOGÍA MOLECULAR DE PLANTAS BIOLOGÍA MOLECULAR DE PLANTAS

Políticas públicas sobre violencia feminicida y feminicidio en México. Una mirada desde las Unidades de Análisis y Contexto

MARISOL ALCOCER PERULERO (2022, [Artículo])

El feminicidio es el asesinato de una mujer ¿ o niña ¿ por el hecho de ser mujer, es decir por razones de género. El término expresa la violencia extrema que se manifiesta en este acto, muchas veces perpetrado con particular crueldad, precedido de maltratos, secuestro, tortura sexual y seguido de la exposición del cadáver en el espacio público. Este texto tiene como objetivo reflexionar respecto al funcionamiento de las Unidades de Análisis y Contexto para la investigación de feminicidios, ¿ creadas en el marco de las Declaratorias de Alerta de Violencia de Género en México (art. 22, LGAMVLV) ¿, para observar las características jurídico-normativas, técnicas y de recursos que tienen y deberían tener de acuerdo con las recomendaciones internacionales. Sostenemos que las unidades de inteligencia y de análisis de contextos son una herramienta para la investigación criminal, que pueden funcionar para comprender el feminicidio y las violencias interseccionales en el país. A través de un análisis descriptivo y documental, privilegiamos los informes oficiales de las Unidades de Análisis y Contexto sobre violencia contra las mujeres, analizamos los principales obstáculos para la implementación de dichas unidades, y desde la perspectiva de género y pertinencia cultural, la forma y estrategias en cómo se capta la información para distinguir entre homicidio y feminicidio, y mujeres indígenas y/o afromexicanas, en un contexto de violencias estructurales y del crimen organizado.

Femicide is the murder of a woman ¿ or girl ¿ due to the fact of being a woman, that is, for reasons of gender. The term expresses the extreme violence that is manifested in this act, often perpetrated with particular cruelty, preceded by mistreatment, kidnapping, sexual torture and followed by the exposition of the corpse in public space. Objective: This text aims to reflect on the functioning of the Analysis and Context Units that have been created in Mexico,to observe the legal-regulatory, technical and resource characteristics that they have and should have in accordance with international recommendations. We sustain that the intelligence and context analysis units are a tool for criminal investigation, which can work to understand intersectional violence in Mexico. Through a descriptive and documentary analysis, we privilege the official reports of the Analysis and Context Units on violence against women, we analyze, based on the main obstacles to the implementation of said units, from the perspective of gender and relevance culture, as well as the way and strategies in how information is collected to distinguish between homicide and femicide, and to identify indigenous and/or Afro-Mexican women, and in a context of other structural violence and organized crime.

Violencia feminicida. Políticas públicas. Interseccionalidad. Análisis de contexto. CIENCIAS SOCIALES CIENCIA POLÍTICA OTRAS ESPECIALIDADES POLÍTICAS