Búsqueda avanzada


Área de conocimiento




160 resultados, página 10 de 10

Draft Genome Sequence of Pediococcus pentosaceus Strain PP16CC, Isolated from Oyster Crassostrea corteziensis

JULIO ANTONIO HERNANDEZ GONZALEZ RICARDO VAZQUEZ JUAREZ Jose Manuel Vazquez-Guillen Carlos Rangel Dávalos MARIA CRISTINA RODRIGUEZ PADILLA MAURILIA ROJAS CONTRERAS (2022, [Artículo])

"Pediococcus pentosaceus strain PP16CC comes from the intestine of Crassostrea corteziensis. A 1.82-Mbp draft genome of this strain was assembled using A5-miseq from illumina reads, resulting in 4 contigs and 1,856 predicted protein coding genes. Additionally, 23 proteins belonging to various glycosyl hydrolase families and 6 prophage regions were identified."

Genome Sequence, Pediococcus pentosaceus BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA BIOLOGÍA ANIMAL (ZOOLOGÍA) GENÉTICA ANIMAL GENÉTICA ANIMAL

Geospatial analysis of the distribution of empty spaces located in Nuevo Laredo, Tamaulipas

Claudio Curzio HECTOR DE LA TORRE GUTIERREZ (2021, [Artículo, Artículo])

Reflect about urban empty spaces means looking around all these lots that are distributed in different geographical points of the cities and are characterized because they are still waiting to be built, therefore, they are vacant, and also these spaces are empty in the sense of not provide any function. In this sense, the objective of this article is to present a methodological proposal specially designed to study this type of urban elements from the particular point of view of urban geography and spatial analysis. Specifically, through a series of surveys, both digital and physical, the various urban voids located in the city of Nuevo Laredo were recorded, thereby creating a database that included a total of 3,836 case studies. In this way, using different types of statistical techniques and spatial models, it was possible to obtain and interpret the results, managing to identify exactly what were the main patterns and characteristics of said geographic distribution.

empty spaces urban vacant lots geo-spatial analysis urban geography border in Nuevo Laredo Vacíos urbanos lotes baldíos análisis geo-espacial geografía urbana frontera en Nuevo Laredo Geografía urbana economía urbana morfología urbana CIENCIAS FÍSICO MATEMÁTICAS Y CIENCIAS DE LA TIERRA CIENCIAS FÍSICO MATEMÁTICAS Y CIENCIAS DE LA TIERRA

Transcriptome mining provides insights into cell wall metabolism and fiber lignification in Agave tequilana Weber

Luis Fernando Maceda Lopez ELSA BEATRIZ GONGORA CASTILLO Enrique Ibarra-Laclette DALIA C. MORAN VELAZQUEZ AMARANTA GIRON RAMIREZ Matthieu Bourdon José Luis Villalpando Aguilar Gabriela Chavez-Calvillo Toomer John Tang Parastoo Azadi Jorge Manuel Santamaría Fernández Itzel López-Rosas Mercedes G Lopez June Simpson FULGENCIO ALATORRE COBOS (2022, [Artículo])

Resilience of growing in arid and semiarid regions and a high capacity of accumulating sugar-rich biomass with low lignin percentages have placed Agave species as an emerging bioen-ergy crop. Although transcriptome sequencing of fiber-producing agave species has been explored, molecular bases that control wall cell biogenesis and metabolism in agave species are still poorly understood. Here, through RNAseq data mining, we reconstructed the cellulose biosynthesis pathway and the phenylpropanoid route producing lignin monomers in A. tequilana, and evaluated their expression patterns in silico and experimentally. Most of the orthologs retrieved showed differential expression levels when they were analyzed in different tissues with contrasting cellulose and lignin accumulation. Phylogenetic and structural motif analyses of putative CESA and CAD proteins allowed to identify those potentially involved with secondary cell wall formation. RT-qPCR assays revealed enhanced expression levels of AtqCAD5 and AtqCESA7 in parenchyma cells associated with extraxylary fibers, suggesting a mechanism of formation of sclerenchyma fibers in Agave similar to that reported for xylem cells in model eudicots. Overall, our results provide a framework for un-derstanding molecular bases underlying cell wall biogenesis in Agave species studying mechanisms involving in leaf fiber development in monocots. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.

AGAVE CELL WALLS LIGNOCELLULOSE CAD PROTEIN CESA PROTEIN SCLERENCHYMA BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA GENÉTICA GENÉTICA MOLECULAR DE PLANTAS GENÉTICA MOLECULAR DE PLANTAS

Ajustar las condiciones de operación de una prensa hidráulica horizontal de fabricación propia para un proceso de doblado en frío a 90°

Hugo Estrada Pimentel HUGO ARCOS GUTIERREZ JOSE ANTONIO BETANCOURT CANTERA Jan Mayén Chaires John Edison García Herrera (2023, [Artículo])

During the cold-bending process, the tooling suffers considerable damage due to excess pressure. This also causes the machines to break down, causing problems in the precision and quality of the metal parts formed. The precision depends on the operating conditions of the press, the tooling employed, and the elastic recovery effect of the material. This study determines the working conditions for a made-in-house horizontal hydraulic press through an experimental design (DOE). This research carried out the V-forming to 90° (ISO 2768-1) of a hot-rolled carbon steel plate, considering pressure, piston permanence time, and recovery factor (Kr). The experimental and statistical analysis ensures accurate forming while the work pressure decreases by 17% and 33%, respectively, regarding the maximum. This reduction will delay the appearance of fatigue damage and have the operating parameters well established; in turn, it will be possible to design tools according to commercial standards.

Durante el proceso de conformado en frío, las herramientas sufren daños considerables debido al exceso de presión. Esto también causa que las máquinas se rompan, causando problemas en la precisión y calidad de las piezas metálicas formadas. La precisión depende de las condiciones de funcionamiento de la prensa, las herramientas empleadas y el efecto elástico de recuperación del material. Este estudio determina las condiciones de trabajo de una prensa hidráulica horizontal fabricado en casa a través de un diseño experimental (DOE). Esta investigación llevó a cabo el conformado en V a 90° (ISO 2768-1) de una placa de acero al carbono laminada en caliente, teniendo en cuenta la presión, el tiempo de permanencia del pistón y el factor de recuperación (Kr). El análisis experimental y estadístico asegura un conformado preciso, mientras que la presión de trabajo disminuyó en un 17% y 33%, respectivamente, con respecto al máximo. Esta reducción retrasará la aparición de daños por fatiga y tendrá los parámetros de funcionamiento bien establecidos; con estos, a su vez, será posible diseñar herramientas de acuerdo con los estándares comerciales.

Authorship acknowledgement: Hugo Estrada: Conceptualization; methodology; validation; writing; revision; project administration; software; investigation; display; draft writing; reviewing and editing. Hugo Arcos: Conceptualization; methodology; validation; writing; revision; simulation; software; reviewing. José A. Betancourt: Supervision; methodology; validation; writing; revision; draft writing: writing reviewing and editing. Jan Mayen: Supervision; methodology; validation; writing; revision; formal analysis.

John E. García: Conceptualization; supervision; methodology; validation; writing; revision; project management; formal analysis, draft writing: writing reviewing and editing.

Cold forming DOE ANOVA Recovery factor (Kr) Factorial analysis 2k Spring back (SB) Conformado en frío Factor de recuperación (Kr) Análisis factorial 2k Recuperación elástica (SB) INGENIERÍA Y TECNOLOGÍA CIENCIAS TECNOLÓGICAS OTRAS ESPECIALIDADES TECNOLÓGICAS OTRAS OTRAS

A twelve years story of waste of social resources: The Mexico-Toluca Interurban Train

Rubén Chavarín (2024, [Artículo, Artículo])

The general objective of this paper is to make an ex-post evaluation of the Mexico-Toluca Interurban Train project (TIMT) more than nine years after the beginning of its construction stage. The present study is based on the cost-benefit analysis approach, through which some analysis scenarios have been built and whose results are contrasted with the official project evaluations. The present research has focused on the analysis of the TIMT as a microcosm of the scarce efficiency of public investment in Mexico, resulting in a waste of social resources. According to the results of the study, the combination of delays and cost overruns (186% in nominal terms, 114% in real terms, and 90% in dollars) has configured a project (across two Federal Government Administrations) with negative social profitability, with losses valued at more than 550 million dollars, placing it in the most problematic quartile of train projects in the world.

cost-benefit analysis ex-post evaluation public investment transport projects Latin America análisis costo-beneficio evaluación ex-post inversión pública proyectos de transporte América Latina CIENCIAS SOCIALES CIENCIAS SOCIALES

The banana MaWRKY18, MaWRKY45, MaWRKY60 and MaWRKY70 genes encode functional transcription factors and display differential expression in response to defense phytohormones

SERGIO GARCIA LAYNES VIRGINIA AURORA HERRERA VALENCIA Lilia Guadalupe Tamayo Torres VERONICA LIMONES BRIONES FELIPE ALONSO BARREDO POOL FRAY MARTIN BAAS ESPINOLA Angel Alpuche-Solis CARLOS ALBERTO PUCH HAU SANTY PERAZA ECHEVERRIA (2022, [Artículo])

WRKY transcription factors (TFs) play key roles in plant defense responses through phytohormone signaling pathways. However, their functions in tropical fruit crops, especially in banana, remain largely unknown. Several WRKY genes from the model plants rice (OsWRKY45) and Arabidopsis (AtWRKY18, AtWRKY60, AtWRKY70) have shown to be attractive TFs for engineering disease resistance. In this study, we isolated four banana cDNAs (MaWRKY18, MaWRKY45, MaWRKY60, and MaWRKY70) with homology to these rice and Arabidopsis WRKY genes. The MaWRKY cDNAs were isolated from the wild banana Musa acuminata ssp. malaccensis, which is resistant to several diseases of this crop and is a progenitor of most banana cultivars. The deduced amino acid sequences of the four MaWRKY cDNAs revealed the presence of the conserved WRKY domain of ~60 amino acids and a zinc-finger motif at the N-terminus. Based on the number of WRKY repeats and the structure of the zinc-finger motif, MaWRKY18 and MaWRKY60 belong to group II of WRKY TFs, while MaWRKY45 and MaWRKY70 are members of group III. Their corresponding proteins were located in the nuclei of onion epidermal cells and were shown to be functional TFs in yeast cells. Moreover, expression analyses revealed that the majority of these MaWRKY genes were upregulated by salicylic acid (SA) or methyl jasmonate (MeJA) phytohormones, although the expression levels were relatively higher with MeJA treatment. The fact that most of these banana WRKY genes were upregulated by SA or MeJA, which are involved in systemic acquired resistance (SAR) or induced systemic resistance (ISR), respectively, make them interesting candidates for bioengineering broad-spectrum resistance in this crop. © 2022 by the authors.

BANANA TRANSCRIPTION FACTOR WRKY DEFENSE PHYTOHORMONES SALICYLIC ACID METHYL JASMONATE SAR ISR BROAD-SPECTRUM RESISTANCE BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA GENÉTICA GENÉTICA MOLECULAR DE PLANTAS GENÉTICA MOLECULAR DE PLANTAS