Bacterial communities in the rhizosphere at different growth stages of maize cultivated in soil under conventional and conservation agricultural practices

Yendi Navarro-Noya Bram Govaerts Nele Verhulst Luc Dendooven (2022, [Artículo])

Farmers in Mexico till soil intensively, remove crop residues for fodder and grow maize often in monoculture. Conservation agriculture (CA), including minimal tillage, crop residue retention and crop diversification, is proposed as a more sustainable alternative. In this study, we determined the effect of agricultural practices and the developing maize rhizosphere on soil bacterial communities. Bulk and maize (Zea mays L.) rhizosphere soil under conventional practices (CP) and CA were sampled during the vegetative, flowering and grain filling stage, and 16S rRNA metabarcoding was used to assess bacterial diversity and community structure. The functional diversity was inferred from the bacterial taxa using PICRUSt. Conservation agriculture positively affected taxonomic and functional diversity compared to CP. The agricultural practice was the most important factor in defining the structure of bacterial communities, even more so than rhizosphere and plant growth stage. The rhizosphere enriched fast growing copiotrophic bacteria, such as Rhizobiales, Sphingomonadales, Xanthomonadales, and Burkholderiales, while in the bulk soil of CP other copiotrophs were enriched, e.g., Halomonas and Bacillus. The bacterial community in the maize bulk soil resembled each other more than in the rhizosphere of CA and CP. The bacterial community structure, and taxonomic and functional diversity in the maize rhizosphere changed with maize development and the differences between the bulk soil and the rhizosphere were more accentuated when the plant aged. Although agricultural practices did not alter the effect of the rhizosphere on the soil bacterial communities in the flowering and grain filling stage, they did in the vegetative stage.

Community Assembly Functional Diversity Intensive Agricultural Practices Plant Microbiome CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA SUSTAINABLE AGRICULTURE TILLAGE SOIL BACTERIA MAIZE

Climate-smart agricultural practices influence the fungal communities and soil properties under major agri-food systems

madhu choudhary ML JAT Parbodh Chander Sharma (2022, [Artículo])

Fungal communities in agricultural soils are assumed to be affected by climate, weather, and anthropogenic activities, and magnitude of their effect depends on the agricultural activities. Therefore, a study was conducted to investigate the impact of the portfolio of management practices on fungal communities and soil physical–chemical properties. The study comprised different climate-smart agriculture (CSA)-based management scenarios (Sc) established on the principles of conservation agriculture (CA), namely, ScI is conventional tillage-based rice–wheat rotation, ScII is partial CA-based rice–wheat–mungbean, ScIII is partial CSA-based rice–wheat–mungbean, ScIV is partial CSA-based maize–wheat–mungbean, and ScV and ScVI are CSA-based scenarios and similar to ScIII and ScIV, respectively, except for fertigation method. All the scenarios were flood irrigated except the ScV and ScVI where water and nitrogen were given through subsurface drip irrigation. Soils of these scenarios were collected from 0 to 15 cm depth and analyzed by Illumina paired-end sequencing of Internal Transcribed Spacer regions (ITS1 and ITS2) for the study of fungal community composition. Analysis of 5 million processed sequences showed a higher Shannon diversity index of 1.47 times and a Simpson index of 1.12 times in maize-based CSA scenarios (ScIV and ScVI) compared with rice-based CSA scenarios (ScIII and ScV). Seven phyla were present in all the scenarios, where Ascomycota was the most abundant phyla and it was followed by Basidiomycota and Zygomycota. Ascomycota was found more abundant in rice-based CSA scenarios as compared to maize-based CSA scenarios. Soil organic carbon and nitrogen were found to be 1.62 and 1.25 times higher in CSA scenarios compared with other scenarios. Bulk density was found highest in farmers' practice (Sc1); however, mean weight diameter and water-stable aggregates were found lowest in ScI. Soil physical, chemical, and biological properties were found better under CSA-based practices, which also increased the wheat grain yield by 12.5% and system yield by 18.8%. These results indicate that bundling/layering of smart agricultural practices over farmers' practices has tremendous effects on soil properties, and hence play an important role in sustaining soil quality/health.

Agriculture Management Fungal Community Diversity Indices Climate-Smart Agricultural Practices CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA AGRICULTURE TILLAGE CLIMATE-SMART AGRICULTURE SOIL ORGANIC CARBON

Conservation agriculture based sustainable intensification: India updates

ML JAT (2021, [Objeto de congreso])

CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA CONSERVATION AGRICULTURE SUSTAINABLE INTENSIFICATION LAND MANAGEMENT TILLAGE PLANT ESTABLISHMENT BIOMASS WATER MANAGEMENT

Application of ammonium to a N limited arable soil enriches a succession of bacteria typically found in the rhizosphere

Yendi Navarro-Noya Marco Luna_Guido Nele Verhulst Bram Govaerts Luc Dendooven (2022, [Artículo])

Crop residue management and tillage are known to affect the soil bacterial community, but when and which bacterial groups are enriched by application of ammonium in soil under different agricultural practices from a semi-arid ecosystem is still poorly understood. Soil was sampled from a long-term agronomic experiment with conventional tilled beds and crop residue retention (CT treatment), permanent beds with crop residue burned (PBB treatment) or retained (PBC) left unfertilized or fertilized with 300 kg urea-N ha-1 and cultivated with wheat (Triticum durum L.)/maize (Zea mays L.) rotation. Soil samples, fertilized or unfertilized, were amended or not (control) with a solution of (NH4)2SO4 (300 kg N ha-1) and were incubated aerobically at 25 ± 2 °C for 56 days, while CO2 emission, mineral N and the bacterial community were monitored. Application of NH4+ significantly increased the C mineralization independent of tillage-residue management or N fertilizer. Oxidation of NH4+ and NO2- was faster in the fertilized soil than in the unfertilized soil. The relative abundance of Nitrosovibrio, the sole ammonium oxidizer detected, was higher in the fertilized than in the unfertilized soil; and similarly, that of Nitrospira, the sole nitrite oxidizer. Application of NH4+ enriched Pseudomonas, Flavisolibacter, Enterobacter and Pseudoxanthomonas in the first week and Rheinheimera, Acinetobacter and Achromobacter between day 7 and 28. The application of ammonium to a soil cultivated with wheat and maize enriched a sequence of bacterial genera characterized as rhizospheric and/or endophytic independent of the application of urea, retention or burning of the crop residue, or tillage.

CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA AMMONIUM CROP RESIDUES WHEAT MAIZE TILLAGE SOIL

Appraisal of complementarity of subsurface drip fertigation and conservation agriculture for physiological performance and water economy of maize

C.M. Parihar Hari Sankar Nayak Dipaka Ranjan Sena Renu Pandey Mahesh Gathala ML JAT (2023, [Artículo])

The Indo-Gangetic Plains (IGP) in north-west (NW) India are facing a severe decline in ground water due to prevalent rice-based cropping systems. To combat this issue, conservation agriculture (CA) with an alternative crop/s, such as maize, is being promoted. Recently, surface drip fertigation has also been evaluated as a viable option to address low-nutrient use efficiency and water scarcity problems for cereals. While the individual benefits of CA and sub-surface drip (SSD) irrigation on water economy are well-established, information regarding their combined effect in cereal-based systems is lacking. Therefore, we conducted a two-year field experiment in maize, under an ongoing CA-based maize-wheat system, to evaluate the complementarity of CA with SSD irrigation through two technological interventions–– CA+ (residue retained CA + SSD), PCA+ (partial CA without residue + SSD) – at different N rates (0, 120 and 150 kg N ha-1) in comparison to traditional furrow irrigated (FI) CA and conventional tillage (CT) at 120 kg N ha-1. Our results showed that CA+ had the highest grain yield (8.2 t ha-1), followed by PCA+ (8.1 t ha-1). The grain yield under CA+ at 150 kg N ha-1 was 27% and 30% higher than CA and CT, respectively. Even at the same N level (120 kg N ha-1), CA+ outperformed CA and CT by 16% and 18%, respectively. The physiological performance of maize also revealed that CA+ based plots with 120 kg N ha-1 had 12% and 3% higher photosynthesis rate at knee-high and silking, respectively compared to FI-CA and CT. Overall, compared to the FI-CA and CT, SSD-based CA+ and PCA+ saved 54% irrigation water and increased water productivity (WP) by more than twice. Similarly, a greater number of split N application through fertigation in PCA+ and CA+ increased agronomic nitrogen use efficiency (NUE) and recover efficiency by 8–19% and 14–25%, respectively. Net returns from PCA+ and CA+ at 150 kg N ha-1 were significantly higher by US$ 491 and 456, respectively than the FI-CA and CT treatments. Therefore, CA coupled with SSD provided tangible benefits in terms of yield, irrigation water saving, WP, NUE and profitability. Efforts should be directed towards increasing farmers’ awareness of the benefits of such promising technology for the cultivating food grains and commercial crops such as maize. Concurrently, government support and strict policies are required to enhance the system adaptability.

Net Returns Subsurface Drip Irrigation Subsurface Drip Fertigation CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA EFFICIENCY GRAIN NITROGEN PHOTOSYNTHESIS PHYSIOLOGY WATER SUPPLY CONSERVATION AGRICULTURE CONVENTIONAL TILLAGE FERTIGATION GROUNDWATER NITROGEN-USE EFFICIENCY WATER PRODUCTIVITY

Enhancing maize yield in a conservation agriculture-based maize (Zea mays)- wheat (Triticum aestivum) system through efficient nitrogen management

C.M. Parihar Hari Sankar Nayak Dipaka Ranjan Sena Shankar Lal Jat Mahesh Gathala Upendra Singh (2023, [Artículo])

This study evaluated the impact of contrasting tillage and nitrogen management options on the growth, yield attributes, and yield of maize (Zea mays L.) in a conservation agriculture (CA)-based maize-wheat (Triticum aestivum L.) system. The field experiment was conducted during the rainy (kharif) seasons of 2020 and 2021 at the research farm of ICAR-Indian Agricultural Research Institute (IARI), New Delhi. The experiment was conducted in a split plot design with three tillage practices [conventional tillage with residue (CT), zero tillage with residue (ZT) and permanent beds with residue (PB)] as main plot treatments and in sub-plots five nitrogen management options [Control (without N fertilization), recommended dose of N @150 kg N/ha, Green Seeker-GS based application of split applied N, N applied as basal through urea super granules-USG + GS based application and 100% basal application of slow release fertilizer (SRF) @150 kg N/ha] with three replications. Results showed that both tillage and nitrogen management options had a significant impact on maize growth, yield attributes, and yield in both seasons. However, time to anthesis and physiological maturity were not significantly affected. Yield attributes were highest in the permanent beds and zero tillage plots, with similar numbers of grains per cob (486.1 and 468.6). The highest leaf area index (LAI) at 60 DAP was observed in PB (5.79), followed by ZT(5.68) and the lowest was recorded in CT (5.25) plots. The highest grain yield (2-year mean basis) was recorded with permanent beds plots (5516 kg/ha), while the lowest

was observed with conventional tillage (4931 kg/ha). Therefore, the study highlights the importance of CA practices for improving maize growth and yield, and suggests that farmers can achieve better results through the adoption of CA-based permanent beds and use of USG as nitrogen management option.

Green Seeker Urea Super Granules CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA MAIZE UREA YIELDS ZERO TILLAGE NITROGEN

Editorial: Conservation agriculture: knowledge frontiers around the world

Stéphane Cordeau ML JAT Cameron Pittelkow Christian Thierfelder (2023, [Artículo])

No-Tillage Direct Seeding Cover Crops Crop Diversification CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA COVER PLANTS DIVERSIFICATION CROPPING SYSTEMS DIRECT SOWING ZERO TILLAGE

Energy and economic efficiency of climate-smart agriculture practices in a rice–wheat cropping system of India

Manish Kakraliya Deepak Bijarniya Parbodh Chander Sharma ML JAT (2022, [Artículo])

Intensive Tillage Conventional Rice–Wheat Systems Energy Efficiency On-Farm Studies Climate-Smart Agricultural Practices CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA CLIMATE-SMART AGRICULTURE RICE WHEAT CROPPING SYSTEMS

Gender dynamics of weed management under conservation agriculture: Insights from the Eastern Indo-Gangetic Plains of South Asia

Hom Nath Gartaula (2021, [Objeto de congreso])

CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA GENDER WEED CONTROL CONSERVATION AGRICULTURE ZERO TILLAGE

Weed management and tillage effect on rainfed maize production in three agro-ecologies in Mexico

Simon Fonteyne Abel Jaime Leal González Rausel Ovando Ravi Gopal Singh Nele Verhulst (2022, [Artículo])

Maize (Zea mays L.) is grown in a wide range of agro-ecological environments and production systems across Mexico. Weeds are a major constraint on maize grain yield, but knowledge regarding the best weed management methods is lacking. In many production systems, reducing tillage could lessen land degradation and production costs, but changes in tillage might require changes in weed management. This study evaluated weed dynamics and rainfed maize yield under five weed management treatments (pre-emergence herbicide, post-emergence herbicide, pre-emergence + post-emergence herbicide, manual weed control, and no control) and three tillage methods (conventional, minimum and zero tillage) in three agro-ecologically distinct regions of the state of Oaxaca, Mexico, in 2016 and 2017. In the temperate Mixteca region, weeds reduced maize grain yields by as much as 92% and the long-growing season required post-emergence weed control, which gave significantly higher yields. In the hot, humid Papaloapan region, weeds reduced maize yields up to 63% and pre-emergence weed control resulted in significantly higher yields than treatments with post-emergence control only. In the semi-arid Valles Centrales region, weeds reduced maize yields by as much as 65%, but weed management was not always effective in increasing maize yield or net profitability. The most effective weed management treatments tended to be similar for the three tillage systems at each site, although weed pressure and the potential yield reduction by weeds tended to be higher under zero tillage than minimum or conventional tillage. No single best option for weed management was found across sites or tillage systems. More research, in which non-chemical methods should not be overlooked, is thus needed to determine the most effective weed management methods for the diverse maize production systems across Mexico.

Corn Integrated Weed Management Manual Weed Control CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA MAIZE WEED CONTROL MINIMUM TILLAGE ZERO TILLAGE